DLRB2_HUMAN
ID DLRB2_HUMAN Reviewed; 96 AA.
AC Q8TF09;
DT 23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Dynein light chain roadblock-type 2;
DE AltName: Full=Dynein light chain 2B, cytoplasmic;
DE AltName: Full=Roadblock domain-containing protein 2;
GN Name=DYNLRB2; Synonyms=DNCL2B, DNLC2B, ROBLD2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Liver, and Testis;
RX PubMed=11750132; DOI=10.1016/s0378-1119(01)00787-9;
RA Jiang J., Yu L., Huang X., Chen X., Li D., Zhang Y., Tang L., Zhao S.;
RT "Identification of two novel human dynein light chain genes, DNLC2A and
RT DNLC2B, and their expression changes in hepatocellular carcinoma tissues
RT from 68 Chinese patients.";
RL Gene 281:103-113(2001).
RN [2]
RP PROTEIN SEQUENCE OF 2-10 AND 55-70, CLEAVAGE OF INITIATOR METHIONINE,
RP ACETYLATION AT ALA-2, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Ovarian carcinoma;
RA Bienvenut W.V., Lempens A., Norman J.C.;
RL Submitted (OCT-2009) to UniProtKB.
RN [3]
RP INTERACTION WITH DYNC1I1 AND DYNC1I2.
RX PubMed=12077152; DOI=10.1074/jbc.m205510200;
RA Susalka S.J., Nikulina K., Salata M.W., Vaughan P.S., King S.M.,
RA Vaughan K.T., Pfister K.K.;
RT "The roadblock light chain binds a novel region of the cytoplasmic Dynein
RT intermediate chain.";
RL J. Biol. Chem. 277:32939-32946(2002).
CC -!- FUNCTION: Acts as one of several non-catalytic accessory components of
CC the cytoplasmic dynein 1 complex that are thought to be involved in
CC linking dynein to cargos and to adapter proteins that regulate dynein
CC function. Cytoplasmic dynein 1 acts as a motor for the intracellular
CC retrograde motility of vesicles and organelles along microtubules.
CC -!- SUBUNIT: Homodimer (Probable). The cytoplasmic dynein 1 complex
CC consists of two catalytic heavy chains (HCs) and a number of non-
CC catalytic subunits presented by intermediate chains (ICs), light
CC intermediate chains (LICs) and light chains (LCs); the composition
CC seems to vary in respect to the IC, LIC and LC composition. The heavy
CC chain homodimer serves as a scaffold for the probable homodimeric
CC assembly of the respective non-catalytic subunits. The ICs and LICs
CC bind directly to the HC dimer and the LCs assemble on the IC dimer.
CC Interacts with DYNC1I1 and DYNC1I2. Self-associates. Interacts with
CC DYNLRB1. {ECO:0000269|PubMed:12077152, ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
CC -!- TISSUE SPECIFICITY: High expression in heart, brain, placenta, skeletal
CC muscle, prostate and small intestine; moderate in kidney, pancreas,
CC spleen, testis, ovary and colon; low in lung, liver, thymus and
CC leukocyte.
CC -!- MISCELLANEOUS: Expression is significantly down-regulated in
CC hepatocellular carcinoma (HCC) patients.
CC -!- SIMILARITY: Belongs to the GAMAD family. {ECO:0000305}.
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DR EMBL; AF125108; AAL75945.1; -; mRNA.
DR CCDS; CCDS10929.1; -.
DR RefSeq; NP_570967.1; NM_130897.2.
DR RefSeq; XP_006721358.1; XM_006721295.3.
DR AlphaFoldDB; Q8TF09; -.
DR SMR; Q8TF09; -.
DR BioGRID; 123714; 12.
DR CORUM; Q8TF09; -.
DR IntAct; Q8TF09; 9.
DR MINT; Q8TF09; -.
DR STRING; 9606.ENSP00000302936; -.
DR GlyGen; Q8TF09; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q8TF09; -.
DR PhosphoSitePlus; Q8TF09; -.
DR BioMuta; DYNLRB2; -.
DR jPOST; Q8TF09; -.
DR MassIVE; Q8TF09; -.
DR MaxQB; Q8TF09; -.
DR PaxDb; Q8TF09; -.
DR PeptideAtlas; Q8TF09; -.
DR PRIDE; Q8TF09; -.
DR ProteomicsDB; 74535; -.
DR TopDownProteomics; Q8TF09; -.
DR Antibodypedia; 55118; 100 antibodies from 21 providers.
DR DNASU; 83657; -.
DR Ensembl; ENST00000305904.11; ENSP00000302936.6; ENSG00000168589.15.
DR GeneID; 83657; -.
DR KEGG; hsa:83657; -.
DR MANE-Select; ENST00000305904.11; ENSP00000302936.6; NM_130897.3; NP_570967.1.
DR UCSC; uc002ffo.3; human.
DR CTD; 83657; -.
DR DisGeNET; 83657; -.
DR GeneCards; DYNLRB2; -.
DR HGNC; HGNC:15467; DYNLRB2.
DR HPA; ENSG00000168589; Group enriched (fallopian tube, testis).
DR MIM; 607168; gene.
DR neXtProt; NX_Q8TF09; -.
DR OpenTargets; ENSG00000168589; -.
DR PharmGKB; PA27437; -.
DR VEuPathDB; HostDB:ENSG00000168589; -.
DR eggNOG; KOG4115; Eukaryota.
DR GeneTree; ENSGT00390000011067; -.
DR HOGENOM; CLU_113002_3_2_1; -.
DR InParanoid; Q8TF09; -.
DR OMA; PVTIIRH; -.
DR PhylomeDB; Q8TF09; -.
DR TreeFam; TF315165; -.
DR PathwayCommons; Q8TF09; -.
DR Reactome; R-HSA-5620924; Intraflagellar transport.
DR SignaLink; Q8TF09; -.
DR BioGRID-ORCS; 83657; 2 hits in 1067 CRISPR screens.
DR ChiTaRS; DYNLRB2; human.
DR GenomeRNAi; 83657; -.
DR Pharos; Q8TF09; Tbio.
DR PRO; PR:Q8TF09; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; Q8TF09; protein.
DR Bgee; ENSG00000168589; Expressed in bronchial epithelial cell and 119 other tissues.
DR ExpressionAtlas; Q8TF09; baseline and differential.
DR Genevisible; Q8TF09; HS.
DR GO; GO:0005813; C:centrosome; IBA:GO_Central.
DR GO; GO:0097542; C:ciliary tip; TAS:Reactome.
DR GO; GO:0005929; C:cilium; TAS:Reactome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005868; C:cytoplasmic dynein complex; ISS:UniProtKB.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0045505; F:dynein intermediate chain binding; IBA:GO_Central.
DR GO; GO:0003777; F:microtubule motor activity; NAS:UniProtKB.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR InterPro; IPR016561; DYNLRB1/2.
DR InterPro; IPR004942; Roadblock/LAMTOR2_dom.
DR Pfam; PF03259; Robl_LC7; 1.
DR PIRSF; PIRSF009998; DLC7; 1.
DR SMART; SM00960; Robl_LC7; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Cytoskeleton; Direct protein sequencing; Dynein;
KW Microtubule; Motor protein; Reference proteome; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|Ref.2"
FT CHAIN 2..96
FT /note="Dynein light chain roadblock-type 2"
FT /id="PRO_0000220958"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000269|Ref.2"
FT VARIANT 14
FT /note="H -> R (in dbSNP:rs13332289)"
FT /id="VAR_049126"
SQ SEQUENCE 96 AA; 10855 MW; 526F073855F14330 CRC64;
MAEVEETLKR IQSHKGVIGT MVVNAEGIPI RTTLDNSTTV QYAGLLHHLT MKAKSTVRDI
DPQNDLTFLR IRSKKHEIMV APDKEYLLIV IQNPCE
