ID   DLRB2_MOUSE             Reviewed;          96 AA.
AC   Q9DAJ5;
DT   23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 137.
DE   RecName: Full=Dynein light chain roadblock-type 2;
DE   AltName: Full=Dynein light chain 2B, cytoplasmic;
GN   Name=Dynlrb2; Synonyms=Dncl2b, Dnlc2b;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=C57BL/6J; TISSUE=Testis;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   SELF-ASSOCIATION, INTERACTION WITH DYNLRB1, AND IDENTIFICATION IN THE
RP   CYTOPLASMIC DYNEIN I COMPLEX.
RX   PubMed=14752807; DOI=10.1002/cm.10172;
RA   Nikulina K., Patel-King R.S., Takebe S., Pfister K.K., King S.M.;
RT   "The Roadblock light chains are ubiquitous components of cytoplasmic dynein
RT   that form homo- and heterodimers.";
RL   Cell Motil. Cytoskeleton 57:233-245(2004).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Acts as one of several non-catalytic accessory components of
CC       the cytoplasmic dynein 1 complex that are thought to be involved in
CC       linking dynein to cargos and to adapter proteins that regulate dynein
CC       function. Cytoplasmic dynein 1 acts as a motor for the intracellular
CC       retrograde motility of vesicles and organelles along microtubules.
CC   -!- SUBUNIT: Homodimer (Probable). The cytoplasmic dynein 1 complex
CC       consists of two catalytic heavy chains (HCs) and a number of non-
CC       catalytic subunits presented by intermediate chains (ICs), light
CC       intermediate chains (LICs) and light chains (LCs); the composition
CC       seems to vary in respect to the IC, LIC and LC composition. The heavy
CC       chain homodimer serves as a scaffold for the probable homodimeric
CC       assembly of the respective non-catalytic subunits. The ICs and LICs
CC       bind directly to the HC dimer and the LCs assemble on the IC dimer.
CC       Interacts with DYNC1I1 and DYNC1I2. Self-associates. Interacts with
CC       DYNLRB1 (By similarity). {ECO:0000250, ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9DAJ5-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9DAJ5-2; Sequence=VSP_007238;
CC   -!- SIMILARITY: Belongs to the GAMAD family. {ECO:0000305}.
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DR   EMBL; AK005789; BAB24240.1; -; mRNA.
DR   EMBL; BC048623; AAH48623.1; -; mRNA.
DR   CCDS; CCDS40487.1; -. [Q9DAJ5-1]
DR   RefSeq; NP_083573.1; NM_029297.1. [Q9DAJ5-1]
DR   AlphaFoldDB; Q9DAJ5; -.
DR   SMR; Q9DAJ5; -.
DR   BioGRID; 217500; 1.
DR   MINT; Q9DAJ5; -.
DR   STRING; 10090.ENSMUSP00000077301; -.
DR   PhosphoSitePlus; Q9DAJ5; -.
DR   MaxQB; Q9DAJ5; -.
DR   PaxDb; Q9DAJ5; -.
DR   PeptideAtlas; Q9DAJ5; -.
DR   PRIDE; Q9DAJ5; -.
DR   ProteomicsDB; 277470; -. [Q9DAJ5-1]
DR   ProteomicsDB; 277471; -. [Q9DAJ5-2]
DR   Antibodypedia; 55118; 100 antibodies from 21 providers.
DR   DNASU; 75465; -.
DR   Ensembl; ENSMUST00000078170; ENSMUSP00000077301; ENSMUSG00000034467. [Q9DAJ5-1]
DR   Ensembl; ENSMUST00000213007; ENSMUSP00000148749; ENSMUSG00000034467. [Q9DAJ5-2]
DR   GeneID; 75465; -.
DR   KEGG; mmu:75465; -.
DR   UCSC; uc009nog.1; mouse. [Q9DAJ5-1]
DR   UCSC; uc009noh.1; mouse. [Q9DAJ5-2]
DR   CTD; 83657; -.
DR   MGI; MGI:1922715; Dynlrb2.
DR   VEuPathDB; HostDB:ENSMUSG00000034467; -.
DR   eggNOG; KOG4115; Eukaryota.
DR   GeneTree; ENSGT00390000011067; -.
DR   HOGENOM; CLU_113002_3_2_1; -.
DR   InParanoid; Q9DAJ5; -.
DR   OMA; PVTIIRH; -.
DR   OrthoDB; 1512428at2759; -.
DR   PhylomeDB; Q9DAJ5; -.
DR   TreeFam; TF315165; -.
DR   Reactome; R-MMU-5620924; Intraflagellar transport.
DR   BioGRID-ORCS; 75465; 1 hit in 73 CRISPR screens.
DR   ChiTaRS; Dynlrb2; mouse.
DR   PRO; PR:Q9DAJ5; -.
DR   Proteomes; UP000000589; Chromosome 8.
DR   RNAct; Q9DAJ5; protein.
DR   Bgee; ENSMUSG00000034467; Expressed in olfactory epithelium and 97 other tissues.
DR   Genevisible; Q9DAJ5; MM.
DR   GO; GO:0005813; C:centrosome; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005868; C:cytoplasmic dynein complex; IDA:UniProtKB.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0045505; F:dynein intermediate chain binding; IBA:GO_Central.
DR   GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR   InterPro; IPR016561; DYNLRB1/2.
DR   InterPro; IPR004942; Roadblock/LAMTOR2_dom.
DR   Pfam; PF03259; Robl_LC7; 1.
DR   PIRSF; PIRSF009998; DLC7; 1.
DR   SMART; SM00960; Robl_LC7; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cytoplasm; Cytoskeleton; Dynein; Microtubule;
KW   Motor protein; Reference proteome; Transport.
FT   CHAIN           1..96
FT                   /note="Dynein light chain roadblock-type 2"
FT                   /id="PRO_0000220959"
FT   VAR_SEQ         83..96
FT                   /note="DKEYLLIVIQNPCE -> EPM (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_007238"
SQ   SEQUENCE   96 AA;  10876 MW;  5C446A9D0E815775 CRC64;
     MTEVEETLKR IQSHKGVIGT MVVNAEGIPI RTTLDNSTTV QYAGLLHQLT MKAKSTVRDI
     DPQNDLTFLR IRSKKHEIMV APDKEYLLIV IQNPCE