DLT1_YEAST
ID DLT1_YEAST Reviewed; 342 AA.
AC Q04216; D6VZU9;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Defect at low temperature protein 1;
GN Name=DLT1; OrderedLocusNames=YMR126C; ORFNames=YM9553.02C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=14968429; DOI=10.1002/yea.1068;
RA Riles L., Shaw R.J., Johnston M., Reines D.;
RT "Large-scale screening of yeast mutants for sensitivity to the IMP
RT dehydrogenase inhibitor 6-azauracil.";
RL Yeast 21:241-248(2004).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=17078969; DOI=10.1016/j.jmb.2006.10.004;
RA White M.A., Clark K.M., Grayhack E.J., Dumont M.E.;
RT "Characteristics affecting expression and solubilization of yeast membrane
RT proteins.";
RL J. Mol. Biol. 365:621-636(2007).
RN [7]
RP FUNCTION.
RX PubMed=18245339; DOI=10.1534/genetics.107.083063;
RA Abe F., Minegishi H.;
RT "Global screening of genes essential for growth in high-pressure and cold
RT environments: searching for basic adaptive strategies using a yeast
RT deletion library.";
RL Genetics 178:851-872(2008).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- FUNCTION: Required for growth under high-pressure and low-temperature
CC conditions. {ECO:0000269|PubMed:18245339}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:17078969}; Multi-
CC pass membrane protein {ECO:0000269|PubMed:17078969}.
CC -!- DISRUPTION PHENOTYPE: Sensitivity to 6-azauracil (6AU) and mycophenolic
CC acid (MPA). {ECO:0000269|PubMed:14968429}.
CC -!- MISCELLANEOUS: Present with 1200 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the DLT1 family. {ECO:0000305}.
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DR EMBL; Z48622; CAA88551.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA10023.1; -; Genomic_DNA.
DR PIR; S53056; S53056.
DR RefSeq; NP_013845.1; NM_001182627.1.
DR AlphaFoldDB; Q04216; -.
DR BioGRID; 35303; 118.
DR IntAct; Q04216; 1.
DR STRING; 4932.YMR126C; -.
DR iPTMnet; Q04216; -.
DR PaxDb; Q04216; -.
DR PRIDE; Q04216; -.
DR EnsemblFungi; YMR126C_mRNA; YMR126C; YMR126C.
DR GeneID; 855156; -.
DR KEGG; sce:YMR126C; -.
DR SGD; S000004733; DLT1.
DR VEuPathDB; FungiDB:YMR126C; -.
DR eggNOG; ENOG502RAJJ; Eukaryota.
DR HOGENOM; CLU_066044_0_0_1; -.
DR InParanoid; Q04216; -.
DR OMA; DRFKFQG; -.
DR BioCyc; YEAST:G3O-32819-MON; -.
DR PRO; PR:Q04216; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q04216; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR038869; DLT1.
DR PANTHER; PTHR40021; PTHR40021; 1.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..342
FT /note="Defect at low temperature protein 1"
FT /id="PRO_0000203299"
FT TOPO_DOM 1..15
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..47
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 69..342
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 342 AA; 39187 MW; C9E6D8726929D3A0 CRC64;
MSGFAKLKSW LYKASLFVSL ILLIGFSVVL PIDSIAQASK SENNAFNTFI VVGALVVFGV
FCIFIIIGRM LFHKSCLKDI PRRYIPITPA DLPHRSSREA VLQNMERSKE LTILLKKPKD
PVIHDGLEPP RRCDYPLDEK LFPEYLNYAD CIKSLTDRLK YHGLFLNNLD VRMNLEDTFA
DVVNSQFVNH NANKIQLEKA KEFIDLYETI RFSGKDVTRD QFIKFVKFCL YFGEVSLTRD
TSFANLHNFR LNGSSNNIGR TESKYSINPF DENEYAQDDM HYFPEPPTHL VRESSISTVA
RHVSSGVDLT NSEEHPLDTD SDCNALRLKL SKADSYRSVI RH
