DLT_ORYSJ
ID DLT_ORYSJ Reviewed; 617 AA.
AC Q9LWU9; A0A1B0NNH8; A3B7Z1; Q8H640;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Protein DWARF AND LOW-TILLERING {ECO:0000303|PubMed:19220793};
DE AltName: Full=GRAS family protein 32 {ECO:0000303|PubMed:15316287};
DE Short=OsGRAS-32 {ECO:0000303|PubMed:15316287};
DE AltName: Full=Protein SMALL ORGAN SIZE 2 {ECO:0000303|PubMed:28069545};
GN Name=DLT {ECO:0000303|PubMed:19220793};
GN Synonyms=GRAS2 {ECO:0000312|EMBL:AGL08204.1},
GN SMOS2 {ECO:0000303|PubMed:28069545};
GN OrderedLocusNames=Os06g0127800 {ECO:0000312|EMBL:BAS95938.1},
GN LOC_Os06g03710 {ECO:0000305};
GN ORFNames=OsJ_19967 {ECO:0000312|EMBL:EAZ35680.1},
GN OSJNBa0038F22.20 {ECO:0000312|EMBL:BAC24836.1},
GN P0425F02.54 {ECO:0000312|EMBL:BAA90816.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Dongjin;
RA Zhou Y., Offler C.E., Patrick J.W.;
RT "Oryza sativa cultivar Dongjin scarecrow-like transcription factor GRAS2
RT (GRAS2) mRNA.";
RL Submitted (JUL-2012) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP GENE FAMILY.
RX PubMed=15316287; DOI=10.1023/b:plan.0000038256.89809.57;
RA Tian C., Wan P., Sun S., Li J., Chen M.;
RT "Genome-wide analysis of the GRAS gene family in rice and Arabidopsis.";
RL Plant Mol. Biol. 54:519-532(2004).
RN [7]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19220793; DOI=10.1111/j.1365-313x.2009.03825.x;
RA Tong H., Jin Y., Liu W., Li F., Fang J., Yin Y., Qian Q., Zhu L., Chu C.;
RT "DWARF AND LOW-TILLERING, a new member of the GRAS family, plays positive
RT roles in brassinosteroid signaling in rice.";
RL Plant J. 58:803-816(2009).
RN [8]
RP FUNCTION, INTERACTION WITH GSK2, SUBCELLULAR LOCATION, AND PHOSPHORYLATION.
RX PubMed=22685166; DOI=10.1105/tpc.112.097394;
RA Tong H., Liu L., Jin Y., Du L., Yin Y., Qian Q., Zhu L., Chu C.;
RT "DWARF AND LOW-TILLERING acts as a direct downstream target of a
RT GSK3/SHAGGY-like kinase to mediate brassinosteroid responses in rice.";
RL Plant Cell 24:2562-2577(2012).
RN [9]
RP FUNCTION, INTERACTION WITH SMOS1, AND DISRUPTION PHENOTYPE.
RX PubMed=28069545; DOI=10.1016/j.molp.2016.12.013;
RA Hirano K., Yoshida H., Aya K., Kawamura M., Hayashi M., Hobo T.,
RA Sato-Izawa K., Kitano H., Ueguchi-Tanaka M., Matsuoka M.;
RT "SMALL ORGAN SIZE 1 and SMALL ORGAN SIZE 2/DWARF AND LOW-TILLERING form a
RT complex to integrate auxin and brassinosteroid signaling in rice.";
RL Mol. Plant 10:590-604(2017).
CC -!- FUNCTION: Probable transcription factor that acts as positivie
CC regulator of brassinosteroid (BR) signaling (PubMed:19220793,
CC PubMed:22685166). Functions downstream of BRI1 and GSK2 to modulate BR
CC responses. Acts as a direct target of GSK2 kinase to mediate BR
CC responses (PubMed:22685166). Involved in feedback inhibition of BR
CC biosynthetic genes. Repressed by BZR1 (PubMed:19220793). Cooperatively
CC functions in a transactivating complex with SMOS1 to enhance the
CC transcription of the SMOS1 target PHI-1, and regulate plant organ size
CC (PubMed:28069545). Interaction between SMOS1 and DLT is a crosstalk
CC point for auxin and brassinosteroid signaling (PubMed:28069545).
CC {ECO:0000269|PubMed:28069545}.
CC -!- SUBUNIT: Interacts with GSK2 (PubMed:22685166). Interacts with SMOS1
CC (via C-terminus) (PubMed:28069545). {ECO:0000269|PubMed:22685166,
CC ECO:0000269|PubMed:28069545}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22685166}.
CC -!- TISSUE SPECIFICITY: Expressed in the shoot apical meristem (SAM) and
CC elongating cells of young seedlings. Expressed in leaf joints, culms,
CC internodes, stems, young panicles, primary roots and lateral roots.
CC {ECO:0000269|PubMed:19220793}.
CC -!- INDUCTION: Down-regulated by 24-epibrassinolide.
CC {ECO:0000269|PubMed:19220793}.
CC -!- PTM: Phosphorylated on serine and threonine residues by GSK2.
CC Dephosphorylated during response to brassinosteroid.
CC {ECO:0000269|PubMed:22685166}.
CC -!- DISRUPTION PHENOTYPE: Semi-dwarf phenotype with small and wide leaves.
CC Erect leaf and panicle phenotype. Reduced lamina joint bending angles.
CC Reduced tiller number and seed set. Reduced brassinosteroid sensitivity
CC and altered expression of brassinosteroid-responsive genes
CC (PubMed:19220793, PubMed:28069545). Decreased cell size, increased cell
CC number, and abnormal cell arrangement and microtubule orientation
CC (PubMed:28069545). {ECO:0000269|PubMed:19220793,
CC ECO:0000269|PubMed:28069545}.
CC -!- SIMILARITY: Belongs to the GRAS family. {ECO:0000305}.
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DR EMBL; JX310319; AGL08204.1; -; mRNA.
DR EMBL; AP001168; BAA90816.1; -; Genomic_DNA.
DR EMBL; AP002838; BAC24836.1; -; Genomic_DNA.
DR EMBL; AP008212; BAF18581.1; -; Genomic_DNA.
DR EMBL; AP014962; BAS95938.1; -; Genomic_DNA.
DR EMBL; CM000143; EAZ35680.1; -; Genomic_DNA.
DR RefSeq; XP_015642886.1; XM_015787400.1.
DR RefSeq; XP_015642887.1; XM_015787401.1.
DR AlphaFoldDB; Q9LWU9; -.
DR SMR; Q9LWU9; -.
DR STRING; 4530.OS06T0127800-01; -.
DR PaxDb; Q9LWU9; -.
DR PRIDE; Q9LWU9; -.
DR EnsemblPlants; Os06t0127800-01; Os06t0127800-01; Os06g0127800.
DR Gramene; Os06t0127800-01; Os06t0127800-01; Os06g0127800.
DR KEGG; osa:4339978; -.
DR eggNOG; ENOG502QU5D; Eukaryota.
DR HOGENOM; CLU_016521_0_0_1; -.
DR InParanoid; Q9LWU9; -.
DR OMA; MYTVSAW; -.
DR PlantReactome; R-OSA-5632095; Brassinosteroid signaling.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000007752; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009742; P:brassinosteroid mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:1900459; P:positive regulation of brassinosteroid mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:0007346; P:regulation of mitotic cell cycle; IEA:EnsemblPlants.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR InterPro; IPR030027; Scarecrow-like_28.
DR InterPro; IPR005202; TF_GRAS.
DR PANTHER; PTHR31636; PTHR31636; 1.
DR PANTHER; PTHR31636:SF16; PTHR31636:SF16; 1.
DR Pfam; PF03514; GRAS; 1.
DR PROSITE; PS50985; GRAS; 1.
PE 1: Evidence at protein level;
KW Brassinosteroid signaling pathway; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..617
FT /note="Protein DWARF AND LOW-TILLERING"
FT /id="PRO_0000439016"
FT DOMAIN 208..594
FT /note="GRAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 29..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 159..206
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 215..295
FT /note="Leucine repeat I (LRI)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 301..366
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 376..408
FT /note="Leucine repeat II (LRII)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 417..508
FT /note="PFYRE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 511..594
FT /note="SAW"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 596..617
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 332..336
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT COMPBIAS 49..63
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 173..187
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 601..617
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 493
FT /note="P -> H (in Ref. 5; EAZ35680)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 617 AA; 65847 MW; C76E64A236297972 CRC64;
MLAGCSFSSS RHQMSTAQRF DILPCGFSKR GSRGDGAAPR VAGDARSGAT TCSFRTHPAP
PVTQSVSWGA KPEPGGNGNG AHRAVKRAHD EDAVEEYGPI VRAKRTRMGG DGDEVWFHQS
IAGTMQATAA GEGEEAEEEK VFLVPSAAAF PHGMAAAGPS LAAAKKEEYS KSPSDSSSSS
GTDGGSSAMM PPPQPPEFDA RNGVPAPGQA EREALELVRA LTACADSLSA GNHEAANYYL
ARLGEMASPA GPTPMHRVAA YFTEALALRV VRMWPHMFDI GPPRELTDDA FGGGDDDAMA
LRILNAITPI PRFLHFTLNE RLLREFEGHE RVHVIDFDIK QGLQWPGLLQ SLAARAVPPA
HVRITGVGES RQELQETGAR LARVAAALGL AFEFHAVVDR LEDVRLWMLH VKRGECVAVN
CVLAMHRLLR DDAALTDFLG LARSTGATIL LLGEHEGGGL NSGRWEARFA RALRYYAAAF
DAVDAAGLPE ASPARAKAEE MFAREIRNAV AFEGPERFER HESFAGWRRR MEDGGGFKNA
GIGEREAMQG RMIARMFGPD KYTVQAHGGG GSGGGEALTL RWLDQPLYTV TAWTPAGDGA
GGSTVSASTT ASHSQQS
