DLX1_MOUSE
ID DLX1_MOUSE Reviewed; 255 AA.
AC Q64317;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Homeobox protein DLX-1;
GN Name=Dlx1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=129;
RX PubMed=8812481; DOI=10.1006/geno.1996.0387;
RA McGuinness T., Porteus M.H., Smiga S., Bulfone A., Kingsley C., Qiu M.,
RA Liu J.K., Long J.E., Xu D., Rubenstein J.L.R.;
RT "Sequence, organization, and transcription of the Dlx-1 and Dlx-2 locus.";
RL Genomics 35:473-485(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 98-255, POSSIBLE FUNCTION, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Brain;
RX PubMed=1676488; DOI=10.1038/351748a0;
RA Price M., Lemaistre M., Pischetola M., di Lauro R., Duboule D.;
RT "A mouse gene related to Distal-less shows a restricted expression in the
RT developing forebrain.";
RL Nature 351:748-751(1991).
RN [4]
RP FUNCTION, INTERACTION WITH POU4F2, AND DEVELOPMENTAL STAGE.
RX PubMed=21875655; DOI=10.1016/j.neuroscience.2011.08.015;
RA Feng L., Eisenstat D.D., Chiba S., Ishizaki Y., Gan L., Shibasaki K.;
RT "Brn-3b inhibits generation of amacrine cells by binding to and negatively
RT regulating DLX1/2 in developing retina.";
RL Neuroscience 195:9-20(2011).
CC -!- FUNCTION: Plays a role as a transcriptional activator or repressor
CC (PubMed:21875655). Inhibits several cytokine signaling pathways, such
CC as TGFB1, activin-A/INHBA and BMP4 by interfering with the
CC transcriptional stimulatory activity of transcription factors, such as
CC MSX2, FAST2, SMAD2 and SMAD3 during hematopoietic cell differentiation
CC (By similarity). Plays a role in terminal differentiation of
CC interneurons, such as amacrine and bipolar cells in the developing
CC retina (PubMed:21875655). Likely to play a regulatory role in the
CC development of the ventral forebrain (PubMed:1676488). May play a role
CC in craniofacial patterning and morphogenesis and may be involved in the
CC early development of diencephalic subdivisions (PubMed:1676488).
CC {ECO:0000250|UniProtKB:P56177, ECO:0000269|PubMed:1676488,
CC ECO:0000269|PubMed:21875655}.
CC -!- SUBUNIT: Interacts with SMAD4 (via homeobox DNA-binding domain) (By
CC similarity). Interacts (via homeobox DNA-binding domain) with POU4F2;
CC this interaction suppresses DLX1-mediated transcriptional activity in
CC postnatal retina and enhances retinal ganglion cell (RGC)
CC differentiation (PubMed:21875655). {ECO:0000250|UniProtKB:P56177,
CC ECO:0000269|PubMed:21875655}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P56177}.
CC -!- TISSUE SPECIFICITY: Expressed in a restricted region of the developing
CC brain, within the diencephalon and the adjacent telencephalic regions.
CC {ECO:0000269|PubMed:1676488}.
CC -!- DEVELOPMENTAL STAGE: Expressed in developing retinal progenitor cells
CC at 12 dpc (PubMed:21875655). Has a segmental expression in the
CC developing forebrain (PubMed:1676488). {ECO:0000269|PubMed:1676488,
CC ECO:0000269|PubMed:21875655}.
CC -!- DOMAIN: The homeobox DNA-binding domain is necessary for its nuclear
CC localization, transcriptional and erythroid differentiation activities.
CC {ECO:0000250|UniProtKB:P56177}.
CC -!- SIMILARITY: Belongs to the distal-less homeobox family. {ECO:0000305}.
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DR EMBL; U51001; AAB40900.1; -; mRNA.
DR EMBL; U51000; AAB40899.1; -; Genomic_DNA.
DR EMBL; BC079609; AAH79609.1; -; mRNA.
DR EMBL; X57552; CAB37647.1; -; Genomic_DNA.
DR CCDS; CCDS16116.1; -.
DR RefSeq; NP_034183.1; NM_010053.2.
DR RefSeq; XP_006498734.1; XM_006498671.3.
DR RefSeq; XP_006498735.1; XM_006498672.3.
DR RefSeq; XP_006498736.1; XM_006498673.3.
DR RefSeq; XP_017170794.1; XM_017315305.1.
DR RefSeq; XP_017170796.1; XM_017315307.1.
DR RefSeq; XP_017170800.1; XM_017315311.1.
DR AlphaFoldDB; Q64317; -.
DR SMR; Q64317; -.
DR BioGRID; 199234; 11.
DR IntAct; Q64317; 10.
DR STRING; 10090.ENSMUSP00000042413; -.
DR PhosphoSitePlus; Q64317; -.
DR MaxQB; Q64317; -.
DR PaxDb; Q64317; -.
DR PeptideAtlas; Q64317; -.
DR PRIDE; Q64317; -.
DR ProteomicsDB; 279725; -.
DR ABCD; Q64317; 1 sequenced antibody.
DR Antibodypedia; 33859; 365 antibodies from 33 providers.
DR DNASU; 13390; -.
DR Ensembl; ENSMUST00000037119; ENSMUSP00000042413; ENSMUSG00000041911.
DR GeneID; 13390; -.
DR KEGG; mmu:13390; -.
DR UCSC; uc008kau.1; mouse.
DR CTD; 1745; -.
DR MGI; MGI:94901; Dlx1.
DR VEuPathDB; HostDB:ENSMUSG00000041911; -.
DR eggNOG; KOG0850; Eukaryota.
DR GeneTree; ENSGT00940000160029; -.
DR HOGENOM; CLU_074733_2_0_1; -.
DR InParanoid; Q64317; -.
DR OMA; MEFGPPG; -.
DR OrthoDB; 1416398at2759; -.
DR PhylomeDB; Q64317; -.
DR TreeFam; TF315720; -.
DR BioGRID-ORCS; 13390; 0 hits in 73 CRISPR screens.
DR ChiTaRS; Dlx1; mouse.
DR PRO; PR:Q64317; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q64317; protein.
DR Bgee; ENSMUSG00000041911; Expressed in medial ganglionic eminence and 182 other tissues.
DR ExpressionAtlas; Q64317; baseline and differential.
DR Genevisible; Q64317; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0003682; F:chromatin binding; IDA:MGI.
DR GO; GO:0003677; F:DNA binding; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0071773; P:cellular response to BMP stimulus; ISS:UniProtKB.
DR GO; GO:0071560; P:cellular response to transforming growth factor beta stimulus; ISS:UniProtKB.
DR GO; GO:0021892; P:cerebral cortex GABAergic interneuron differentiation; IGI:MGI.
DR GO; GO:0021893; P:cerebral cortex GABAergic interneuron fate commitment; IGI:MGI.
DR GO; GO:0048706; P:embryonic skeletal system development; IMP:MGI.
DR GO; GO:0021879; P:forebrain neuron differentiation; IGI:MGI.
DR GO; GO:0097154; P:GABAergic neuron differentiation; IGI:MGI.
DR GO; GO:0021766; P:hippocampus development; IGI:MGI.
DR GO; GO:0030514; P:negative regulation of BMP signaling pathway; ISS:UniProtKB.
DR GO; GO:1903845; P:negative regulation of cellular response to transforming growth factor beta stimulus; ISS:UniProtKB.
DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; IMP:MGI.
DR GO; GO:0045746; P:negative regulation of Notch signaling pathway; IGI:MGI.
DR GO; GO:0048715; P:negative regulation of oligodendrocyte differentiation; IGI:MGI.
DR GO; GO:0046533; P:negative regulation of photoreceptor cell differentiation; IMP:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IGI:MGI.
DR GO; GO:0014016; P:neuroblast differentiation; IMP:MGI.
DR GO; GO:0051402; P:neuron apoptotic process; IMP:MGI.
DR GO; GO:0007219; P:Notch signaling pathway; IGI:MGI.
DR GO; GO:0042475; P:odontogenesis of dentin-containing tooth; IGI:MGI.
DR GO; GO:0048709; P:oligodendrocyte differentiation; IGI:MGI.
DR GO; GO:1902871; P:positive regulation of amacrine cell differentiation; IMP:UniProtKB.
DR GO; GO:0045597; P:positive regulation of cell differentiation; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0009954; P:proximal/distal pattern formation; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0021544; P:subpallium development; IGI:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR InterPro; IPR000047; HTH_motif.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; Differentiation; DNA-binding; Homeobox;
KW Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..255
FT /note="Homeobox protein DLX-1"
FT /id="PRO_0000049021"
FT DNA_BIND 128..187
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 95..118
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 204..233
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 218..233
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 98..103
FT /note="QSRLED -> PEPPGG (in Ref. 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 123
FT /note="G -> GQ (in Ref. 3)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 255 AA; 27309 MW; 99B474F5D3FD8B6D CRC64;
MTMTTMPESL NSPVSGKAVF MEFGPPNQQM SPSPMSHGHY SMHCLHSAGH SQPDGAYSSA
SSFSRPLGYP YVNSVSSHAS SPYISSVQSY PGSASLAQSR LEDPGADSEK STVVEGGEVR
FNGKGKKIRK PRTIYSSLQL QALNRRFQQT QYLALPERAE LAASLGLTQT QVKIWFQNKR
SKFKKLMKQG GAALEGSALA NGRALSAGSP PVPPGWNPNS SSGKGSGSSA GSYVPSYTSW
YPSAHQEAMQ QPQLM
