ID   ADC_CLOBE               Reviewed;         246 AA.
AC   Q9RPK1; Q9RPJ8;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Acetoacetate decarboxylase {ECO:0000255|HAMAP-Rule:MF_00597};
DE            Short=AAD {ECO:0000255|HAMAP-Rule:MF_00597};
DE            Short=ADC {ECO:0000255|HAMAP-Rule:MF_00597};
DE            EC=4.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00597};
GN   Name=adc {ECO:0000255|HAMAP-Rule:MF_00597};
OS   Clostridium beijerinckii (Clostridium MP).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1520;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NRRL B-593;
RA   Toth J., Chen J.S.;
RT   "Organization of the solvent-production genes in Clostridium beijerinckii
RT   NRRL B593.";
RL   Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NRRL B-593;
RA   Toth J., Colby G.D., Chen J.S.;
RT   "Acetoacetate:butyrate/acetate coenzyme A transferase and its structural
RT   genes from Clostridium beijerinckii NRRL B593.";
RL   Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the conversion of acetoacetate to acetone and
CC       carbon dioxide. {ECO:0000255|HAMAP-Rule:MF_00597}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetoacetate + H(+) = acetone + CO2; Xref=Rhea:RHEA:19729,
CC         ChEBI:CHEBI:13705, ChEBI:CHEBI:15347, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16526; EC=4.1.1.4; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00597};
CC   -!- SIMILARITY: Belongs to the ADC family. {ECO:0000255|HAMAP-
CC       Rule:MF_00597}.
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DR   EMBL; AF157305; AAD54946.1; -; Genomic_DNA.
DR   EMBL; AF157306; AAD54949.2; -; Genomic_DNA.
DR   AlphaFoldDB; Q9RPK1; -.
DR   SMR; Q9RPK1; -.
DR   STRING; 1520.LF65_04320; -.
DR   GO; GO:0047602; F:acetoacetate decarboxylase activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.40.400.10; -; 1.
DR   HAMAP; MF_00597; ADC; 1.
DR   InterPro; IPR010451; Acetoacetate_decarboxylase.
DR   InterPro; IPR023653; Acetoacetate_decarboxylase_bac.
DR   InterPro; IPR023375; ADC_dom_sf.
DR   Pfam; PF06314; ADC; 1.
DR   SUPFAM; SSF160104; SSF160104; 1.
PE   3: Inferred from homology;
KW   Decarboxylase; Lyase; Schiff base.
FT   CHAIN           1..246
FT                   /note="Acetoacetate decarboxylase"
FT                   /id="PRO_0000207102"
FT   ACT_SITE        115
FT                   /note="Schiff-base intermediate with acetoacetate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00597"
SQ   SEQUENCE   246 AA;  27354 MW;  79FEEA01185E7C98 CRC64;
     MLESEVSKQI TTPLAAPAFP RGPYRFHNRE YLNIIYRTDL DALRKIVPEP LELDGAYVRF
     EMMAMPDTTG LGSYTECGQA IPVKYNEVKG DYLHMMYLDN EPAIAVGRES SAYPKKFGYP
     KLFVDSDALV GALKYGALPV VTATMGYKHE PLDLKEAYTQ IARPNFMLKI IQGYDGKPRI
     CELICAENTD ITIHGAWTGS ARLQLFSHAL APLADLPVLE IVSASHILTD LTLGTPKVVH
     DYLSVK