DLX5_RAT
ID DLX5_RAT Reviewed; 289 AA.
AC P50575;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 25-MAY-2022, entry version 145.
DE RecName: Full=Homeobox protein DLX-5;
DE AltName: Full=Homeobox protein DLX-3;
DE AltName: Full=RDLX;
GN Name=Dlx5;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=7915995; DOI=10.1016/0014-5793(94)00896-5;
RA Shirasawa T., Sakamoto K., Takahashi H.;
RT "Molecular cloning and evolutional analysis of a mammalian homologue of the
RT Distal-less 3 (Dlx-3) homeobox gene.";
RL FEBS Lett. 351:380-384(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley; TISSUE=Cartilage;
RX PubMed=7913069; DOI=10.1006/dbio.1994.1178;
RA Zhao G.-Q., Zhao S., Zhou X., Eberspaecher H., Solursh M.,
RA de Crombrugghe B.;
RT "rDlx, a novel distal-less-like homeoprotein is expressed in developing
RT cartilages and discrete neuronal tissues.";
RL Dev. Biol. 164:37-51(1994).
CC -!- FUNCTION: Transcriptional factor involved in bone development. Acts as
CC an immediate early BMP-responsive transcriptional activator essential
CC for osteoblast differentiation. Stimulates ALPL promoter activity in a
CC RUNX2-independent manner during osteoblast differentiation. Stimulates
CC SP7 promoter activity during osteoblast differentiation. Promotes cell
CC proliferation by up-regulating MYC promoter activity. Involved as a
CC positive regulator of both chondrogenesis and chondrocyte hypertrophy
CC in the endochondral skeleton. Binds to the homeodomain-response element
CC of the ALPL and SP7 promoter. Binds to the MYC promoter. Requires the
CC 5'-TAATTA-3' consensus sequence for DNA-binding (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with XRCC6 (Ku70). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC -!- TISSUE SPECIFICITY: Mainly expressed in several neuronal tissues and
CC developing tissues.
CC -!- DEVELOPMENTAL STAGE: Present at high levels in embryos on embryonic day
CC 14 (E14), in skeletal tissues on E18, and in adult brain. At lower
CC levels in newborn rib cartilage, embryo soft tissues on E18, newborn
CC skin and adult heart.
CC -!- PTM: Phosphorylated. Phosphorylation of Ser-34 and Ser-217 by MAPK14
CC enhances its transcriptional activity. Phosphorylation by CaMK2
CC increases its protein stability (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the distal-less homeobox family. {ECO:0000305}.
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DR EMBL; D31734; BAA06534.1; -; mRNA.
DR EMBL; L24443; AAA42026.1; -; mRNA.
DR PIR; I53082; I53082.
DR PIR; S48664; S48664.
DR RefSeq; NP_037075.1; NM_012943.1.
DR AlphaFoldDB; P50575; -.
DR BMRB; P50575; -.
DR SMR; P50575; -.
DR PaxDb; P50575; -.
DR GeneID; 25431; -.
DR KEGG; rno:25431; -.
DR UCSC; RGD:2506; rat.
DR CTD; 1749; -.
DR RGD; 2506; Dlx5.
DR eggNOG; KOG0850; Eukaryota.
DR InParanoid; P50575; -.
DR OrthoDB; 1416398at2759; -.
DR PhylomeDB; P50575; -.
DR TreeFam; TF350606; -.
DR PRO; PR:P50575; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0000785; C:chromatin; ISO:RGD.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0005634; C:nucleus; ISO:RGD.
DR GO; GO:0003677; F:DNA binding; ISO:RGD.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:RGD.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; TAS:RGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0071837; F:HMG box domain binding; ISO:RGD.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:RGD.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:RGD.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; ISS:UniProtKB.
DR GO; GO:0048646; P:anatomical structure formation involved in morphogenesis; ISO:RGD.
DR GO; GO:0007411; P:axon guidance; ISO:RGD.
DR GO; GO:0007409; P:axonogenesis; ISO:RGD.
DR GO; GO:0030509; P:BMP signaling pathway; ISO:RGD.
DR GO; GO:0060349; P:bone morphogenesis; ISO:RGD.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0008283; P:cell population proliferation; ISS:UniProtKB.
DR GO; GO:0071773; P:cellular response to BMP stimulus; ISO:RGD.
DR GO; GO:0043583; P:ear development; ISO:RGD.
DR GO; GO:0030326; P:embryonic limb morphogenesis; ISO:RGD.
DR GO; GO:0001958; P:endochondral ossification; ISS:UniProtKB.
DR GO; GO:0030855; P:epithelial cell differentiation; ISO:RGD.
DR GO; GO:0060325; P:face morphogenesis; ISO:RGD.
DR GO; GO:0060322; P:head development; ISO:RGD.
DR GO; GO:0042472; P:inner ear morphogenesis; ISO:RGD.
DR GO; GO:0097376; P:interneuron axon guidance; ISO:RGD.
DR GO; GO:0021889; P:olfactory bulb interneuron differentiation; ISO:RGD.
DR GO; GO:0060166; P:olfactory pit development; ISO:RGD.
DR GO; GO:0001649; P:osteoblast differentiation; ISS:UniProtKB.
DR GO; GO:0090263; P:positive regulation of canonical Wnt signaling pathway; ISO:RGD.
DR GO; GO:0050679; P:positive regulation of epithelial cell proliferation; ISO:RGD.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:RGD.
DR GO; GO:0045669; P:positive regulation of osteoblast differentiation; IMP:RGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:1901522; P:positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus; ISO:RGD.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0060021; P:roof of mouth development; ISO:RGD.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR022135; Distal-less_N.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR InterPro; IPR000047; HTH_motif.
DR Pfam; PF12413; DLL_N; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; DNA-binding; Homeobox; Nucleus;
KW Osteogenesis; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..289
FT /note="Homeobox protein DLX-5"
FT /id="PRO_0000049033"
FT DNA_BIND 137..196
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 198..253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 270..289
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 25..47
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 203..253
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 34
FT /note="Phosphoserine; by MAPK14; in vitro"
FT /evidence="ECO:0000250|UniProtKB:P70396"
FT MOD_RES 217
FT /note="Phosphoserine; by MAPK14; in vitro"
FT /evidence="ECO:0000250|UniProtKB:P70396"
FT CONFLICT 67
FT /note="G -> R (in Ref. 2; AAA42026)"
FT /evidence="ECO:0000305"
FT CONFLICT 242
FT /note="P -> T (in Ref. 2; AAA42026)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 289 AA; 31426 MW; A1B41BB44FD15DA2 CRC64;
MTGVFDRRVP SIRSGDFQAP FPTSAAMHHP SQESPTLPES SATDSDYYSP AGAAPHGYCS
PTSASYGKAL NPYQYQYHSV NGSAAGYPAK AYADYGYASP YHQYGGAYNR VPSATSQPEK
EVAEPEVRMV NGKPKKVRKP RTIYSSFQLA ALQRRFQKTQ YLALPERAEL AASLGLTQTQ
VKIWFQNKRS KIKKIMKNGE MPPEHSPSSS DPMACNSPQS PAVWEPQGSS RSLSHHPHAH
PPTSNQSPAS SYLENSASWY PSAASSINSH LPPPGSLQHP LALASGTLY
