ADDA_ACET2
ID ADDA_ACET2 Reviewed; 1251 AA.
AC A3DH19;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=ATP-dependent helicase/nuclease subunit A {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01451};
DE AltName: Full=ATP-dependent helicase/nuclease AddA {ECO:0000255|HAMAP-Rule:MF_01451};
GN Name=addA {ECO:0000255|HAMAP-Rule:MF_01451}; OrderedLocusNames=Cthe_2039;
OS Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC
OS 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC Acetivibrio.
OX NCBI_TaxID=203119;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D.,
RA Newcomb M., Richardson P.;
RT "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC and an ATP-dependent, dual-direction single-stranded exonuclease.
CC Recognizes the chi site generating a DNA molecule suitable for the
CC initiation of homologous recombination. The AddA nuclease domain is
CC required for chi fragment generation; this subunit has the helicase and
CC 3' -> 5' nuclease activities. {ECO:0000255|HAMAP-Rule:MF_01451}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- SUBUNIT: Heterodimer of AddA and AddB/RexB. {ECO:0000255|HAMAP-
CC Rule:MF_01451}.
CC -!- SIMILARITY: Belongs to the helicase family. AddA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01451}.
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DR EMBL; CP000568; ABN53248.1; -; Genomic_DNA.
DR RefSeq; WP_020457694.1; NC_009012.1.
DR AlphaFoldDB; A3DH19; -.
DR SMR; A3DH19; -.
DR STRING; 203119.Cthe_2039; -.
DR PRIDE; A3DH19; -.
DR EnsemblBacteria; ABN53248; ABN53248; Cthe_2039.
DR KEGG; cth:Cthe_2039; -.
DR eggNOG; COG1074; Bacteria.
DR HOGENOM; CLU_001114_3_1_9; -.
DR OMA; KQSIYRW; -.
DR OrthoDB; 137860at2; -.
DR Proteomes; UP000002145; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 4.
DR Gene3D; 3.90.320.10; -; 1.
DR HAMAP; MF_01451; AddA; 1.
DR InterPro; IPR014152; DNA_helicase_suAddA.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR PANTHER; PTHR11070:SF48; PTHR11070:SF48; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
DR TIGRFAMs; TIGR02785; addA_Gpos; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Exonuclease; Helicase;
KW Hydrolase; Nuclease; Nucleotide-binding; Reference proteome.
FT CHAIN 1..1251
FT /note="ATP-dependent helicase/nuclease subunit A"
FT /id="PRO_0000379265"
FT DOMAIN 5..481
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT DOMAIN 526..824
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT REGION 544..565
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 26..33
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
SQ SEQUENCE 1251 AA; 143346 MW; 615624C9539ACD73 CRC64;
MSERTKWTDE QWEAITGNEK SLLVSAAAGA GKTAVLVERI IRKITDEENP VDIDRLLVVT
FTNAAATEMR ERIAQAISEK LEENPGSANI QRQLTLLGKA CITTIHSFCL EVIRSNFQQI
NIDPGFRIAD ETESRLMKLE ALDEVFEEQY ENENEDFFEL LECYGGNRDD RALQDMVLNL
YDFIQSSPWP EEWLEKMTES MNIPDGTDFG KTLWGSVLLS SVKIELEGLK EMISRALEIL
KDASGLEKYR AVYMEDLANV DALLKLLNEE SEMQWDRIFN ALQGFEFATL PRCGREVDKD
KQEIVKKIRD DVKQRIRKFR EKVITSVSNE IISDLKALYP KMKCLANLVK QLAEKYAEKK
NRKSVVDFND LEHFCLEILT ERKEDGSIIP SRTAISYRER FAEILVDEYQ DSNLVQETII
NMISKGDDAS PGVFMVGDVK QSIYRFRQAR PELFLEKYNT YLPDKGSPCR KIILSRNFRS
RREVIDAVNF LFKQIMSTGA GELDYTDAEA LNFGAVFDEN AKEDITVGGE VEFHLIQTED
EDKNFTFENE GEEGRQADEG EEDEEMLDSI QCEARLVGRR ILELMKPDEN GRYFSVFDKA
KNEYRRVEYR DVVILLRTTR NWAEVFVDEL SVMGIPVFAD TGTGFFKTVE VQVMLSLLQI
IDNPLQDIPL LSVLRSPIVG FTTDELAELR LVDKKALLFD ALKKLAESGQ GEAAGKASAF
LENLQKWREM SLYMSTDRLL WQLYNDTGYY SIVGAMPAGE QRQANLRILY ERARQFEETS
YKGLFNFINF IDKLKSSRGD MGSAKILSEN DNVVRIMSIH KSKGLEFPVV IVAGCGKKFN
LQDMNKSILL HHELGFGPDV VDHKLRLSWP SVAKQAIREK IKAETLSEEM RILYVALTRA
REKLVITGAV KNVRKAVEKW LDSASVQKSR LSAYDMLSGA NYLDWIGPAL LRHKNCGGLR
DCVGSAGFRG LLIDDPSVWS VKIWNKTDVQ SSGVSEEQGE SEFIKWLDSL EKEEPSEYAE
ETARRLSWSY PYVKASKVPA KVSVTELKRR YNEVVSEDVM QFPDYMPVLV KKPMFLEEKK
GLTYAEKGTI LHFVMQHLDY GREDIEAQIE EMVAKDLLTP QQAQSVDAAR IRRFLNSPLG
KRMLASKSIN REVPFNIEIP CHELYRDMED EACHGETLLL QGVVDCYFEE PDGIVLVDYK
TDYVAPGNVE TIRERYKVQI LYYARALEML TGKKVKEKYI YLFWDGRILG F
