ID   DMFAB_PARAH             Reviewed;         762 AA.
AC   C9DQ21;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   03-NOV-2009, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=N,N-dimethylformamidase beta subunit {ECO:0000250|UniProtKB:Q9LCC0};
DE            EC=3.5.1.56;
DE   AltName: Full=N,N-dimethylformamidase heavy chain {ECO:0000250|UniProtKB:Q9LCC0};
GN   Name=dmfA2 {ECO:0000312|EMBL:ACV81784.1};
OS   Paracoccus aminophilus.
OG   Plasmid pAMI2 {ECO:0000312|EMBL:ACV81784.1}.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Paracoccus.
OX   NCBI_TaxID=34003;
RN   [1] {ECO:0000312|EMBL:ACV81784.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 49673 / DSM 8538 / JCM 7686 / NBRC 16710;
RX   PubMed=17158670; DOI=10.1128/jb.01610-06;
RA   Dziewit L., Jazurek M., Drewniak L., Baj J., Bartosik D.;
RT   "The SXT conjugative element and linear prophage N15 encode toxin-
RT   antitoxin-stabilizing systems homologous to the tad-ata module of the
RT   Paracoccus aminophilus plasmid pAMI2.";
RL   J. Bacteriol. 189:1983-1997(2007).
RN   [2] {ECO:0000305, ECO:0000312|EMBL:ACV81784.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC   STRAIN=ATCC 49673 / DSM 8538 / JCM 7686 / NBRC 16710;
RX   PubMed=20118371; DOI=10.1128/aem.01926-09;
RA   Dziewit L., Dmowski M., Baj J., Bartosik D.;
RT   "Plasmid pAMI2 of Paracoccus aminophilus JCM 7686 carries N,N-
RT   dimethylformamide degradation-related genes whose expression is activated
RT   by a LuxR family regulator.";
RL   Appl. Environ. Microbiol. 76:1861-1869(2010).
CC   -!- FUNCTION: Hydrolyzes N,N-dimethylformamide, and to a lesser extent N,N-
CC       dimethylacetamide and N,N-diethylacetamide. Has no activity against the
CC       substituted amides N-methylformamide, N-ethylformamide, N-
CC       ethylformamide and N-methylacetamide or the unsubstituted amides
CC       formamide, nicotinamide, acetoamide, benzamide, acetamide and
CC       acrylamide (By similarity). {ECO:0000250|UniProtKB:Q9LCC0}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + N,N-dimethylformamide = dimethylamine + formate;
CC         Xref=Rhea:RHEA:19517, ChEBI:CHEBI:15377, ChEBI:CHEBI:15740,
CC         ChEBI:CHEBI:17741, ChEBI:CHEBI:58040; EC=3.5.1.56;
CC         Evidence={ECO:0000250|UniProtKB:Q9LCC0};
CC   -!- SUBUNIT: Heterotetramer of two DmfA1 (alpha) and two DmfA2 (beta)
CC       subunits. {ECO:0000250}.
CC   -!- INDUCTION: By N,N-dimethylformamide. {ECO:0000269|PubMed:20118371}.
CC   -!- CAUTION: It is not known which subunit of DmfA1 or DmfA2 possesses
CC       catalytic activity. {ECO:0000305}.
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DR   EMBL; GQ410978; ACV81784.1; -; Genomic_DNA.
DR   RefSeq; WP_012476890.1; NC_010847.2.
DR   RefSeq; YP_003208118.1; NC_010847.2.
DR   AlphaFoldDB; C9DQ21; -.
DR   SMR; C9DQ21; -.
DR   GO; GO:0050116; F:N,N-dimethylformamidase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   SUPFAM; SSF49899; SSF49899; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Plasmid.
FT   CHAIN           1..762
FT                   /note="N,N-dimethylformamidase beta subunit"
FT                   /id="PRO_0000404196"
SQ   SEQUENCE   762 AA;  84394 MW;  5BD0C376B7110940 CRC64;
     MKTVKIRGYC DRLSAAPGET IRFYVSADTS DGSYEAELVR LIHGDTNPAG PGYKEESVKS
     AADGSYPARF QRTQFGSFVE VPDASGALLA DGAFSVHTFL WSTTPGRGRQ GLVSRWDDER
     QCGWSLAIEE GRLVFTIGDE SGTTNRVISD RPLFQEVWYS VTAVFDPVQK TISLHQKSVV
     NRTNSRFGLV VPLDSDTTVS AVSQVSPGDS RTSLLIAGLG EAAAADGRTW CIANFNGKID
     APKLYGRALS SEEAMKLAEG TVAEPWSRLA HWDFSAGIGP DGIPTDHVVD ISGNGHHGQC
     VNQPDRGSTG WNWDGHEENF IHCPQQYGAL WFHEDCLDDC RWDKDFEITL PDGLKSDFYA
     MKIRYGDSED YIPFFVLPPR GKATAKILVL ASTFSYLAYA NEQIMHKADI GQAVAGHTPV
     LNENDVELHR NLDYYGLSTY DGHVDGRGVQ YTSWRRPILN LRPKHRQGFG SIWELPADLH
     LIDWLNHNGF DYDVATEHDL NEQGVDLLRR YNVVLTGSHP EYQTWANADA WEDYLADGGR
     GMYLAANGMY WIVSVHPEKP WVMEVRKELG VTAWEAPPGE YHYSTNGRRG GRFRGRARAT
     QKIWGTGMSS FGFDHSGYFV QMPDSQDKRA AWIMDGIDPD ERIGDGGLVG GGAGGYELDR
     YDLSLGTPPN TLLLASSVEH SVVYTVIPDD KSFPHPGMNG GEHPFVRADI TYFSTANGGG
     MFSTSSISWL GSLSWNNYDN NVSRMTRNVL TQFMKDEPAP LV