DMKN_MOUSE
ID DMKN_MOUSE Reviewed; 517 AA.
AC Q6P253; Q6E0U6; Q6SZJ9; Q9D1D0;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 2.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Dermokine;
DE AltName: Full=Epidermis-specific secreted protein SK30/SK89;
DE Flags: Precursor;
GN Name=Dmkn;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RC STRAIN=CD-1;
RX PubMed=15256262; DOI=10.1016/j.gene.2004.03.010;
RA Moffatt P., Salois P., St Amant N., Gaumond M.-H., Lanctot C.;
RT "Identification of a conserved cluster of skin-specific genes encoding
RT secreted proteins.";
RL Gene 334:123-131(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4), TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND FUNCTION.
RC STRAIN=BALB/cJ; TISSUE=Skin;
RX PubMed=15234001; DOI=10.1016/j.ygeno.2004.03.010;
RA Matsui T., Hayashi-Kisumi F., Kinoshita Y., Katahira S., Morita K.,
RA Miyachi Y., Ono Y., Imai T., Tanigawa Y., Komiya T., Tsukita S.;
RT "Identification of novel keratinocyte-secreted peptides dermokine-alpha/-
RT beta and a new stratified epithelium-secreted protein gene complex on human
RT chromosome 19q13.1.";
RL Genomics 84:384-397(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May act as a soluble regulator of keratinocyte
CC differentiation. {ECO:0000305}.
CC -!- SUBUNIT: Homooligomer. Seems to be able to homodimerize and
CC homotrimerize (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15256262}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1; Synonyms=Dermokine-beta;
CC IsoId=Q6P253-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6P253-2; Sequence=VSP_033108, VSP_033109;
CC Name=3; Synonyms=Dermokine-alpha;
CC IsoId=Q6P253-3; Sequence=VSP_033107, VSP_033110;
CC Name=4;
CC IsoId=Q6P253-4; Sequence=VSP_033109;
CC -!- TISSUE SPECIFICITY: Highly expressed in stratified epithelia; such as
CC the skin, tongue, esophagus, forestomach and vagina. Also found in
CC lung, trachea and urinary bladder. {ECO:0000269|PubMed:15234001,
CC ECO:0000269|PubMed:15256262}.
CC -!- DEVELOPMENTAL STAGE: Expression begins during the period of
CC stratification at 15.5 dpc, increases up to 17.5 dpc and decreases at
CC 18.5 dpc. {ECO:0000269|PubMed:15234001}.
CC -!- PTM: O-glycosylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the dermokine family. {ECO:0000305}.
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DR EMBL; AY444557; AAR20796.1; -; mRNA.
DR EMBL; AY444558; AAR20797.1; -; mRNA.
DR EMBL; AY622962; AAT68266.1; -; mRNA.
DR EMBL; AY622963; AAT68267.1; -; mRNA.
DR EMBL; AK003695; BAB22943.1; -; mRNA.
DR EMBL; BC064724; AAH64724.1; -; mRNA.
DR CCDS; CCDS39892.1; -. [Q6P253-4]
DR CCDS; CCDS39893.1; -. [Q6P253-3]
DR CCDS; CCDS52184.1; -. [Q6P253-2]
DR RefSeq; NP_001159646.1; NM_001166174.1.
DR RefSeq; NP_082894.1; NM_028618.2. [Q6P253-3]
DR RefSeq; NP_766487.3; NM_172899.4.
DR AlphaFoldDB; Q6P253; -.
DR STRING; 10090.ENSMUSP00000129031; -.
DR PhosphoSitePlus; Q6P253; -.
DR CPTAC; non-CPTAC-3292; -.
DR MaxQB; Q6P253; -.
DR PeptideAtlas; Q6P253; -.
DR PRIDE; Q6P253; -.
DR ProteomicsDB; 279726; -. [Q6P253-1]
DR ProteomicsDB; 279727; -. [Q6P253-2]
DR ProteomicsDB; 279728; -. [Q6P253-3]
DR ProteomicsDB; 279729; -. [Q6P253-4]
DR Antibodypedia; 29370; 110 antibodies from 20 providers.
DR Ensembl; ENSMUST00000041703; ENSMUSP00000042300; ENSMUSG00000060962. [Q6P253-3]
DR GeneID; 73712; -.
DR KEGG; mmu:73712; -.
DR UCSC; uc009ggk.2; mouse. [Q6P253-3]
DR CTD; 93099; -.
DR MGI; MGI:1920962; Dmkn.
DR VEuPathDB; HostDB:ENSMUSG00000060962; -.
DR eggNOG; ENOG502QQ65; Eukaryota.
DR GeneTree; ENSGT00570000079107; -.
DR HOGENOM; CLU_189557_0_0_1; -.
DR InParanoid; Q6P253; -.
DR PhylomeDB; Q6P253; -.
DR BioGRID-ORCS; 73712; 2 hits in 68 CRISPR screens.
DR ChiTaRS; Dmkn; mouse.
DR PRO; PR:Q6P253; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q6P253; protein.
DR Bgee; ENSMUSG00000060962; Expressed in tail skin and 120 other tissues.
DR ExpressionAtlas; Q6P253; baseline and differential.
DR Genevisible; Q6P253; MM.
DR GO; GO:0005615; C:extracellular space; IDA:MGI.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:1903575; P:cornified envelope assembly; IMP:MGI.
DR InterPro; IPR033541; Dermokine.
DR PANTHER; PTHR36881; PTHR36881; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Differentiation; Glycoprotein; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000250"
FT CHAIN 22..517
FT /note="Dermokine"
FT /id="PRO_0000330765"
FT REGION 51..83
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 123..364
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 143..170
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 194..335
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 350..364
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..408
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15256262,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_033107"
FT VAR_SEQ 291..305
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15256262"
FT /id="VSP_033108"
FT VAR_SEQ 314..322
FT /note="Missing (in isoform 2 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:15234001,
FT ECO:0000303|PubMed:15256262, ECO:0000303|PubMed:15489334"
FT /id="VSP_033109"
FT VAR_SEQ 409..438
FT /note="SPSTRALLYFRKLWENFKRSTPFFNWKQIE -> MKPVTASALLLILLGVAW
FT RGDSHSW (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15256262,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_033110"
FT CONFLICT 88
FT /note="E -> D (in Ref. 1; AAR20796)"
FT /evidence="ECO:0000305"
FT CONFLICT 107
FT /note="R -> K (in Ref. 1; AAR20796)"
FT /evidence="ECO:0000305"
FT CONFLICT 118
FT /note="D -> N (in Ref. 1; AAR20796)"
FT /evidence="ECO:0000305"
FT CONFLICT 134
FT /note="A -> P (in Ref. 1; AAR20796)"
FT /evidence="ECO:0000305"
FT CONFLICT 179
FT /note="D -> N (in Ref. 1; AAR20796 and 2; AAT68267)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 517 AA; 51661 MW; 3FCEDECAA2B5E222 CRC64;
MKLQGSLACL LLALCLGGGA ANPLHSGGEG TGASAAHGAG DAISHGIGEA VGQGAKEAAS
SGIQNALGQG HGEEGGSTLM GSRGDVFEHR LGEAARSLGN AGNEIGRQAE DIIRQGVDAV
HNAGSWGTSG GHGAYGSQGG AGVQGNPGPQ GTPWASGGNY GTNSLGGSVG QGGNGGPLDY
ETNAQGAVAQ PGYGTVRGNN QNSGCTNPPP SGSHESFSNS GGSSNDGSRG SQGSHGSNGQ
GSSGRGGGQG NSDNNGSSSS SSGSNSGNSN SGNSGNSNSG NSGNSGSGSR DIETSNFDEG
YSVSRGTGSR GGSGGSGGSG GSGGSGGSGG GNKPECNNPG NDVRMAGGSG SQGHGSNGGN
IQKEAVNGLN TMNSDASTLP FNIDNFWENL KSKTRFINWD AINKGHAPSP STRALLYFRK
LWENFKRSTP FFNWKQIEGS DLSSLQKRAG GADQFSKPEA RQDLSADSSK NYYNNQQVNP
TYNWQYYTKT TAKAGVTPSS SSASRAQPGL LKWLKFW
