ID   ADDA_ALKOO              Reviewed;        1197 AA.
AC   A8MJ41;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=ATP-dependent helicase/nuclease subunit A {ECO:0000255|HAMAP-Rule:MF_01451};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01451};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01451};
DE   AltName: Full=ATP-dependent helicase/nuclease AddA {ECO:0000255|HAMAP-Rule:MF_01451};
GN   Name=addA {ECO:0000255|HAMAP-Rule:MF_01451}; OrderedLocusNames=Clos_2290;
OS   Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain
OS   OhILAs)).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Alkaliphilus.
OX   NCBI_TaxID=350688;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=OhILAs;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E.,
RA   Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P.;
RT   "Complete genome of Alkaliphilus oremlandii OhILAs.";
RL   Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC       and an ATP-dependent, dual-direction single-stranded exonuclease.
CC       Recognizes the chi site generating a DNA molecule suitable for the
CC       initiation of homologous recombination. The AddA nuclease domain is
CC       required for chi fragment generation; this subunit has the helicase and
CC       3' -> 5' nuclease activities. {ECO:0000255|HAMAP-Rule:MF_01451}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC   -!- SUBUNIT: Heterodimer of AddA and AddB/RexB. {ECO:0000255|HAMAP-
CC       Rule:MF_01451}.
CC   -!- SIMILARITY: Belongs to the helicase family. AddA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
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DR   EMBL; CP000853; ABW19823.1; -; Genomic_DNA.
DR   RefSeq; WP_012160130.1; NC_009922.1.
DR   AlphaFoldDB; A8MJ41; -.
DR   SMR; A8MJ41; -.
DR   STRING; 350688.Clos_2290; -.
DR   PRIDE; A8MJ41; -.
DR   EnsemblBacteria; ABW19823; ABW19823; Clos_2290.
DR   KEGG; aoe:Clos_2290; -.
DR   eggNOG; COG1074; Bacteria.
DR   HOGENOM; CLU_001114_3_1_9; -.
DR   OMA; KQSIYRW; -.
DR   OrthoDB; 137860at2; -.
DR   Proteomes; UP000000269; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.300; -; 4.
DR   Gene3D; 3.90.320.10; -; 1.
DR   HAMAP; MF_01451; AddA; 1.
DR   InterPro; IPR014152; DNA_helicase_suAddA.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR011604; PDDEXK-like_dom_sf.
DR   InterPro; IPR038726; PDDEXK_AddAB-type.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   PANTHER; PTHR11070:SF48; PTHR11070:SF48; 1.
DR   Pfam; PF12705; PDDEXK_1; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF52980; SSF52980; 1.
DR   TIGRFAMs; TIGR02785; addA_Gpos; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA damage; DNA repair; DNA-binding; Exonuclease; Helicase;
KW   Hydrolase; Nuclease; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..1197
FT                   /note="ATP-dependent helicase/nuclease subunit A"
FT                   /id="PRO_0000379228"
FT   DOMAIN          2..458
FT                   /note="UvrD-like helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT   DOMAIN          485..774
FT                   /note="UvrD-like helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT   BINDING         23..30
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
SQ   SEQUENCE   1197 AA;  138760 MW;  8C286E572C08BB13 CRC64;
     MRQWTKEQQA AIDARGSNLL VAAAAGSGKT AVLVERIIQI ILKDRIDIDR LLIVTFTNAA
     AGEMRERIAG AIMTEMEKKT GGEEHLRRQL SLLNRASITT VHSFCIDVVR RHFHMIDVDP
     GFRIGDVTET SIMKLEALEE LFEDEYEKGN EEFFNLVEAF GGTREDRPLQ DLVLKIYGFI
     QSQPYPEIWL RERVEDFALS IEAFNQSPWI RTIKKRMGIL LKGAMDLLEI AQTIALEPGG
     PDVYEEAILS DLDQIKELYH SLENPITDFY EQLNCINFIR LKTSKDSDPI LKEECKDLRD
     KAKDIVKDIR ENIFNVSPEE YVEDLNRLYP LMDYLYRLVI GFTERYTEKK TDKGIVDFND
     LEHFALRILA NDLAAQEYRE KFEYIFVDEY QDSNIVQETL IQSIKREDNL FMVGDVKQSI
     YRFRLADPTL FIEKYETFGT EEGHINRRID LAKNFRSRGQ VLNGVNYIFK HMMSKELGEI
     DYDERAALYQ GTEFEPIQDP SIEINLIEKN MEIDEEMEEE LQELADIEVE ARIVAKRIKA
     LLNEEIYDPR VEGYRKIEYK DIVVLLRTTQ NWAQSFLEVF VREGIPAYAD ANTGYFEAIE
     VNMFLNLLRV IDNKRQDIPL LSVMRSPIGD FTTEELIHIR VNDKTGTYYD AIEKYVEEKT
     DDLKYKLVSF IEKLNQWAND ARYIKIDQFI WKLFMDTGYY YYVGAMPGGL QRQANLRVLF
     DRANQFEKTS IKGLFNFIKF IEKLQSSKGD MGAAKILGEN DNVVRIMSIH KSKGLEFPVV
     ITAGMGKNFN LRDTSADVLL HKDLGLGPKF TDPNLRTYRD TIAKLAMKDQ IKIESLSEEM
     RILYVAFTRP KDKLIVVGSI RNIEKQVKKW SKADNVYSLM NAKNYLDWIG TALVKHPDGQ
     ALRELGGLGF DAASDGMEDS EWTINILGRQ VIVLEEQDKV LKEEDYREKL LYFNREDFSS
     GGYTEYKEEI ENRLNWKYDH PHSVQIPSKL SVSDIKKAHI NEIDVIAHQI PILVKSPKFM
     EGKTTFTAAE RGTVIHFVLQ HLDLNKVGSE EDIREQIHWM VARELITEEE SKVVDTKKIL
     NYFYSPIGER MRKAKKVYRE SPFIIEKSAG EVIEGLSDDI EDKLLVQGII DCYFEEKDGL
     ILIDYKNDIV LNGNIAAVVA RYELQLSLYR EALERITGRE VKETYLYLFD VDQGVRL