ID   DMLR_ECOLI              Reviewed;         307 AA.
AC   P76250;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 2.
DT   03-AUG-2022, entry version 135.
DE   RecName: Full=HTH-type transcriptional regulator DmlR;
DE   AltName: Full=D-malate degradation protein R;
GN   Name=dmlR; Synonyms=yeaT; OrderedLocusNames=b1799, JW1788;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA   Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA   Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA   Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA   Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA   Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA   Horiuchi T.;
RT   "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 40.1-50.0 min region on the linkage map.";
RL   DNA Res. 3:379-392(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   DISRUPTION PHENOTYPE, AND ROLE IN D-MALATE UTILIZATION.
RX   PubMed=17088549; DOI=10.1073/pnas.0603364103;
RA   Reed J.L., Patel T.R., Chen K.H., Joyce A.R., Applebee M.K., Herring C.D.,
RA   Bui O.T., Knight E.M., Fong S.S., Palsson B.O.;
RT   "Systems approach to refining genome annotation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:17480-17484(2006).
RN   [5]
RP   FUNCTION AS A TRANSCRIPTIONAL REGULATOR, ROLE IN D-MALATE UTILIZATION,
RP   INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX   PubMed=20233924; DOI=10.1128/jb.01665-09;
RA   Lukas H., Reimann J., Kim O.B., Grimpo J., Unden G.;
RT   "Regulation of aerobic and anaerobic D-malate metabolism of Escherichia
RT   coli by the LysR-type regulator DmlR (YeaT).";
RL   J. Bacteriol. 192:2503-2511(2010).
CC   -!- FUNCTION: Transcriptional regulator required for the aerobic growth on
CC       D-malate as the sole carbon source. Induces the expression of dmlA in
CC       response to D-malate or L- or meso-tartrate. Negatively regulates its
CC       own expression. {ECO:0000269|PubMed:17088549,
CC       ECO:0000269|PubMed:20233924}.
CC   -!- INDUCTION: Expression of dmlR is subject to negative autoregulation
CC       under both aerobic and anaerobic growth conditions. Its level of
CC       expression is generally lower under anaerobic conditions than that
CC       under aerobic conditions. {ECO:0000269|PubMed:20233924}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of dmlR inhibits aerobic growth on D-
CC       malate and inhibits the induction of dmlA expression by D-malate and
CC       L- and meso-tartrate. {ECO:0000269|PubMed:17088549,
CC       ECO:0000269|PubMed:20233924}.
CC   -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; U00096; AAC74869.2; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15594.1; -; Genomic_DNA.
DR   PIR; G64940; G64940.
DR   RefSeq; NP_416313.4; NC_000913.3.
DR   RefSeq; WP_001061575.1; NZ_LN832404.1.
DR   AlphaFoldDB; P76250; -.
DR   SMR; P76250; -.
DR   BioGRID; 4260334; 12.
DR   IntAct; P76250; 13.
DR   STRING; 511145.b1799; -.
DR   jPOST; P76250; -.
DR   PaxDb; P76250; -.
DR   PRIDE; P76250; -.
DR   EnsemblBacteria; AAC74869; AAC74869; b1799.
DR   EnsemblBacteria; BAA15594; BAA15594; BAA15594.
DR   GeneID; 946316; -.
DR   KEGG; ecj:JW1788; -.
DR   KEGG; eco:b1799; -.
DR   PATRIC; fig|1411691.4.peg.454; -.
DR   EchoBASE; EB3279; -.
DR   eggNOG; COG0583; Bacteria.
DR   HOGENOM; CLU_039613_16_4_6; -.
DR   InParanoid; P76250; -.
DR   OMA; PAFISKR; -.
DR   PhylomeDB; P76250; -.
DR   BioCyc; EcoCyc:G6985-MON; -.
DR   PRO; PR:P76250; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:UniProtKB.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0006108; P:malate metabolic process; IMP:UniProtKB.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR   GO; GO:0006351; P:transcription, DNA-templated; IBA:GO_Central.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR005119; LysR_subst-bd.
DR   InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00126; HTH_1; 1.
DR   Pfam; PF03466; LysR_substrate; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50931; HTH_LYSR; 1.
PE   1: Evidence at protein level;
KW   Activator; DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..307
FT                   /note="HTH-type transcriptional regulator DmlR"
FT                   /id="PRO_0000105786"
FT   DOMAIN          5..62
FT                   /note="HTH lysR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT   DNA_BIND        22..41
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
SQ   SEQUENCE   307 AA;  34631 MW;  608ACFB1873AA364 CRC64;
     MNNLPLLNDL RVFMLVARRA GFAAVAEELG VSPAFVSKRI ALLEQTLNVV LLHRTTRRVT
     ITEEGERIYE WAQRILQDVG QMMDELSDVR QVPQGMLRII SSFGFGRQVV APALLALAKA
     YPQLELRFDV EDRLVDLVNE GVDLDIRIGD DIAPNLIARK LATNYRILCA SPEFIAQHGA
     PKHLTDLSAL PCLVIKERDH PFGVWQLRNK EGPHAIKVTG PLSSNHGEIV HQWCLDGQGI
     ALRSWWDVSE NIASGHLVQV LPEYYQPANV WAVYVSRLAT SAKVRITVEF LRQYFAEHYP
     NFSLEHA