ID   DMPD_PSEUF              Reviewed;         283 AA.
AC   P19076;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=2-hydroxymuconate semialdehyde hydrolase;
DE            Short=HMSH;
DE            EC=3.7.1.9;
DE   AltName: Full=2-hydroxymuconic semialdehyde hydrolase;
GN   Name=dmpD;
OS   Pseudomonas sp. (strain CF600).
OG   Plasmid pVI150.
OC   Bacteria; Proteobacteria.
OX   NCBI_TaxID=79676;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2194577; DOI=10.1016/0167-4781(90)90046-5;
RA   Nordlund I., Shingler V.;
RT   "Nucleotide sequences of the meta-cleavage pathway enzymes 2-hydroxymuconic
RT   semialdehyde dehydrogenase and 2-hydroxymuconic semialdehyde hydrolase from
RT   Pseudomonas CF600.";
RL   Biochim. Biophys. Acta 1049:227-230(1990).
CC   -!- FUNCTION: Catalyzes the conversion of 2-hydroxymuconate semialdehyde to
CC       2-hydroxypent-2,4-dienoate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + H2O = 2-oxopent-4-
CC         enoate + formate + H(+); Xref=Rhea:RHEA:14549, ChEBI:CHEBI:11641,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740,
CC         ChEBI:CHEBI:71198; EC=3.7.1.9;
CC   -!- PATHWAY: Aromatic compound metabolism; benzoate degradation via
CC       hydroxylation.
CC   -!- SIMILARITY: Belongs to the DmpD/TodF/XylF esterase family.
CC       {ECO:0000305}.
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DR   EMBL; X52805; CAA36993.1; -; Genomic_DNA.
DR   AlphaFoldDB; P19076; -.
DR   SMR; P19076; -.
DR   ESTHER; psesp-dmpd; Carbon-carbon_bond_hydrolase.
DR   KEGG; ag:CAA36993; -.
DR   UniPathway; UPA00156; -.
DR   GO; GO:0018775; F:2-hydroxymuconate-semialdehyde hydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0043640; P:benzoate catabolic process via hydroxylation; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR000639; Epox_hydrolase-like.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   PRINTS; PR00111; ABHYDROLASE.
DR   PRINTS; PR00412; EPOXHYDRLASE.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Hydrolase; Plasmid; Serine esterase.
FT   CHAIN           1..283
FT                   /note="2-hydroxymuconate semialdehyde hydrolase"
FT                   /id="PRO_0000207055"
FT   DOMAIN          32..262
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        107
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        228
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        256
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   283 AA;  30965 MW;  D583193818E7E58F CRC64;
     MNAPQNSPEI GREIIAAGIR TNLHDSGAGF PLMMIHGSGP GVTAWANWRL VMPELAKSRR
     VIAPDMLGFG YSERPADAQY NRDVWVDHAV GVLDALEIEQ ADLVGNSFGG GIALALAIRH
     PERVRRLVLM GSAGVSFPIT EGLDAVWGYN PSFAEMRRLL DIFAFDRNLV NDELAELRYQ
     ASIRPGFHES FAAMFPAPRQ RWVDGLASAE AAIRALPHET LVIHGREDQI IPLQTSLTLA
     DWIARAQLHV FGQCGHWTQI EHAARFASLV GDFLAEADAA AIS