DMPK_PSEUF
ID DMPK_PSEUF Reviewed; 92 AA.
AC P19729;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Phenol 2-monooxygenase, auxiliary component DmpK {ECO:0000305};
DE AltName: Full=Phenol 2-monooxygenase P0 component;
DE AltName: Full=Phenol hydroxylase P0 protein;
GN Name=dmpK {ECO:0000303|PubMed:2254258}; Synonyms=pheA1;
OS Pseudomonas sp. (strain CF600).
OG Plasmid pVI150.
OC Bacteria; Proteobacteria.
OX NCBI_TaxID=79676;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PATHWAY, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=CF600;
RX PubMed=2254258; DOI=10.1128/jb.172.12.6826-6833.1990;
RA Nordlund I., Powlowski J., Shingler V.;
RT "Complete nucleotide sequence and polypeptide analysis of multicomponent
RT phenol hydroxylase from Pseudomonas sp. strain CF600.";
RL J. Bacteriol. 172:6826-6833(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=BH;
RA Takeo M., Maeda Y., Okada H., Miyama K., Mori K., Ike M., Fujita M.;
RL Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION.
RC STRAIN=CF600;
RX PubMed=2254259; DOI=10.1128/jb.172.12.6834-6840.1990;
RA Powlowski J., Shingler V.;
RT "In vitro analysis of polypeptide requirements of multicomponent phenol
RT hydroxylase from Pseudomonas sp. strain CF600.";
RL J. Bacteriol. 172:6834-6840(1990).
RN [4]
RP FUNCTION, SUBUNIT, INTERACTION WITH DMPL AND DMPN, AND MASS SPECTROMETRY.
RC STRAIN=CF600;
RX PubMed=8995386; DOI=10.1074/jbc.272.2.945;
RA Powlowski J., Sealy J., Shingler V., Cadieux E.;
RT "On the role of DmpK, an auxiliary protein associated with multicomponent
RT phenol hydroxylase from Pseudomonas sp. strain CF600.";
RL J. Biol. Chem. 272:945-951(1997).
CC -!- FUNCTION: DmpK is an auxiliary protein associated with the
CC multicomponent phenol hydroxylase DmpLMNOP and it may be involved in
CC the post-translational incorporation of iron into the oxygenase
CC component of the phenol hydroxylase (PubMed:8995386). Required for
CC growth on phenol but not for in vitro phenol hydroxylase activity
CC (PubMed:2254258, PubMed:2254259). {ECO:0000269|PubMed:2254258,
CC ECO:0000269|PubMed:2254259, ECO:0000269|PubMed:8995386}.
CC -!- PATHWAY: Aromatic compound metabolism; phenol degradation.
CC {ECO:0000269|PubMed:2254258}.
CC -!- SUBUNIT: Homotrimer or homotetramer (PubMed:8995386). Interacts with
CC the phenol hydroxylase components DmpL (P1 component) and DmpN (P3
CC component) (PubMed:8995386). {ECO:0000269|PubMed:8995386}.
CC -!- MASS SPECTROMETRY: Mass=10451; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:8995386};
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene cannot grow on phenol.
CC {ECO:0000269|PubMed:2254258}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M60276; AAA25939.1; -; Genomic_DNA.
DR EMBL; D28864; BAA06014.1; -; Genomic_DNA.
DR AlphaFoldDB; P19729; -.
DR BioCyc; MetaCyc:MON-12794; -.
DR BRENDA; 1.14.13.244; 16277.
DR UniPathway; UPA00728; -.
DR GO; GO:0018662; F:phenol 2-monooxygenase activity; IEA:UniProtKB-EC.
DR GO; GO:0019336; P:phenol-containing compound catabolic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR010353; DmpK.
DR Pfam; PF06099; Phenol_hyd_sub; 1.
DR PIRSF; PIRSF000039; Phenol_monooxy_K; 1.
PE 1: Evidence at protein level;
KW Aromatic hydrocarbons catabolism; Plasmid.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:8995386"
FT CHAIN 2..92
FT /note="Phenol 2-monooxygenase, auxiliary component DmpK"
FT /id="PRO_0000079940"
SQ SEQUENCE 92 AA; 10586 MW; 9893DFD3CAACB71E CRC64;
MTVTNTPTPT FDQLTRYIRV RSEPEAKFVE FDFAIGHPEL FVELVLPQDA FVKFCQHNRV
VAMDEAMAKA VDDDMVKWRF GDVGRRLPKD PG
