ID   DMPL_ACICP              Reviewed;         332 AA.
AC   Q7WTJ6; F0KIC2;
DT   15-DEC-2003, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Phenol 2-monooxygenase, oxygenase component MhpL {ECO:0000305};
DE            EC=1.14.13.244 {ECO:0000250|UniProtKB:P19730};
DE   AltName: Full=Phenol 2-monooxygenase P1 component;
DE   AltName: Full=Phenol hydroxylase P1 protein;
GN   Name=mphL; OrderedLocusNames=BDGL_000474;
OS   Acinetobacter calcoaceticus (strain PHEA-2).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Moraxellales; Moraxellaceae;
OC   Acinetobacter; Acinetobacter calcoaceticus/baumannii complex.
OX   NCBI_TaxID=871585;
RN   [1] {ECO:0000312|EMBL:CAD92312.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PHEA-2 {ECO:0000312|EMBL:CAD92312.1};
RX   PubMed=12732969; DOI=10.1007/s00284-002-3840-4;
RA   Xu Y., Chen M., Zhang W., Lin M.;
RT   "Genetic organization of genes encoding phenol hydroxylase, benzoate 1,2-
RT   dioxygenase alpha subunit and its regulatory proteins in Acinetobacter
RT   calcoaceticus PHEA-2.";
RL   Curr. Microbiol. 46:235-240(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PHEA-2;
RX   PubMed=21441526; DOI=10.1128/jb.00261-11;
RA   Zhan Y., Yan Y., Zhang W., Yu H., Chen M., Lu W., Ping S., Peng Z.,
RA   Yuan M., Zhou Z., Elmerich C., Lin M.;
RT   "Genome sequence of Acinetobacter calcoaceticus PHEA-2, isolated from
RT   industry wastewater.";
RL   J. Bacteriol. 193:2672-2673(2011).
RN   [3] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 2-16.
RC   STRAIN=69-V;
RA   Benndorf D., Loffhagen N., Hartig C., Babel W.;
RT   "Growth on phenol at chemostress levels amplifies the expression of the
RT   phenol degradation pathway in Acinetobacter calcoaceticus.";
RL   Eng. Life Sci. 4:38-42(2004).
CC   -!- FUNCTION: Part of a multicomponent enzyme which catalyzes the
CC       degradation of phenol and some of its methylated derivatives.
CC       {ECO:0000250|UniProtKB:P19730}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + NADH + O2 + phenol = catechol + H2O + NAD(+);
CC         Xref=Rhea:RHEA:57952, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:15882, ChEBI:CHEBI:18135,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.14.13.244;
CC         Evidence={ECO:0000250|UniProtKB:P19730};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57953;
CC         Evidence={ECO:0000250|UniProtKB:P19730};
CC   -!- PATHWAY: Aromatic compound metabolism; phenol degradation.
CC       {ECO:0000250|UniProtKB:P19730}.
CC   -!- INDUCTION: By phenol. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the TmoE/XamoE family. {ECO:0000305}.
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DR   EMBL; AJ564846; CAD92312.1; -; Genomic_DNA.
DR   EMBL; CP002177; ADY81060.1; -; Genomic_DNA.
DR   RefSeq; WP_014206218.1; NC_016603.1.
DR   RefSeq; YP_004994742.1; NC_016603.1.
DR   AlphaFoldDB; Q7WTJ6; -.
DR   SMR; Q7WTJ6; -.
DR   STRING; 871585.BDGL_000474; -.
DR   PRIDE; Q7WTJ6; -.
DR   EnsemblBacteria; ADY81060; ADY81060; BDGL_000474.
DR   GeneID; 11638652; -.
DR   KEGG; acc:BDGL_000474; -.
DR   PATRIC; fig|871585.3.peg.471; -.
DR   eggNOG; ENOG502Z7Z9; Bacteria.
DR   HOGENOM; CLU_833678_0_0_6; -.
DR   OMA; QGWIYGF; -.
DR   UniPathway; UPA00728; -.
DR   Proteomes; UP000007477; Chromosome.
DR   GO; GO:0018662; F:phenol 2-monooxygenase activity; ISS:UniProtKB.
DR   GO; GO:0046191; P:aerobic phenol-containing compound catabolic process; ISS:UniProtKB.
DR   CDD; cd01058; AAMH_B; 1.
DR   Gene3D; 1.10.620.20; -; 1.
DR   InterPro; IPR009078; Ferritin-like_SF.
DR   InterPro; IPR012078; MP_mOase_hydro.
DR   InterPro; IPR003430; Phenol_Hydrox.
DR   InterPro; IPR012348; RNR-like.
DR   Pfam; PF02332; Phenol_Hydrox; 1.
DR   PIRSF; PIRSF000040; MMOH_comp; 1.
DR   SUPFAM; SSF47240; SSF47240; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; Direct protein sequencing; Monooxygenase;
KW   NAD; Oxidoreductase; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|Ref.3"
FT   CHAIN           2..332
FT                   /note="Phenol 2-monooxygenase, oxygenase component MhpL"
FT                   /id="PRO_0000079941"
SQ   SEQUENCE   332 AA;  38226 MW;  FCAE249C720A0BA6 CRC64;
     MTLEIKTSNV EPIRQNYAYI ERRFGSKPAT RYQEVSFDVQ AETNFHYRPL WKPEKTLNDK
     THTALQMQDW YAFKDPRQFY YGTYVQHRAR LQDTAESNFA FFEKRQLAEH LSNEVKAKVI
     ECLLPFRHVE QTANLHMMSG SAYGYGTVLT QACIYAAMDH LGIAQYISRI GLALDGNSGD
     SLQQAKQAWM QHPAWQGLRR LCEESLTEQD YFKLFLLQNL VIDGFVTELV YQQFDQWLVS
     QNARDLAMLT EFMKDTLGDL RKWSDTVIKT AAAESDHNKQ LLNEWFTESL AQVKAAFTPW
     ATAALTVDAV DQAEQAVIER AKKLGLQPLT NA