DMRT3_MOUSE
ID DMRT3_MOUSE Reviewed; 476 AA.
AC Q80WT2; Q8CGX1;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Doublesex- and mab-3-related transcription factor 3;
GN Name=Dmrt3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP DEVELOPMENTAL STAGE.
RX PubMed=12609607; DOI=10.1016/s1567-133x(02)00071-6;
RA Kim S., Kettlewell J.R., Anderson R.C., Bardwell V.J., Zarkower D.;
RT "Sexually dimorphic expression of multiple doublesex-related genes in the
RT embryonic mouse gonad.";
RL Gene Expr. Patterns 3:77-82(2003).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=22932389; DOI=10.1038/nature11399;
RA Andersson L.S., Larhammar M., Memic F., Wootz H., Schwochow D.,
RA Rubin C.-J., Patra K., Arnason T., Wellbring L., Hjalm G., Imsland F.,
RA Petersen J.L., McCue M.E., Mickelson J.R., Cothran G., Ahituv N.,
RA Roepstorff L., Mikko S., Vallstedt A., Lindgren G., Andersson L.,
RA Kullander K.;
RT "Mutations in DMRT3 affect locomotion in horses and spinal circuit function
RT in mice.";
RL Nature 488:642-646(2012).
CC -!- FUNCTION: Probable transcription factor that plays a role in
CC configuring the spinal circuits controlling stride in vertebrates.
CC Involved in neuronal specification within specific subdivision of
CC spinal cord neurons and in the development of a coordinated locomotor
CC network controlling limb movements. May regulate transcription during
CC sexual development. {ECO:0000269|PubMed:22932389}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00070}.
CC -!- TISSUE SPECIFICITY: Expressed in the ventral spinal cord, in a
CC restrical population of neurons migrating ventrically in the developing
CC spinal cord at 11.5 dpc. {ECO:0000269|PubMed:22932389}.
CC -!- DEVELOPMENTAL STAGE: Expressed at 14.5 dpc in testis, kidney and
CC bladder. Expression drops at 13.5 dpc in female gonads and is not
CC detected at 15.5 dpc while it is expressed by Sertoli cells in testis.
CC {ECO:0000269|PubMed:12609607}.
CC -!- DOMAIN: DMA domain interacts with ubiquitin.
CC {ECO:0000250|UniProtKB:Q9NQL9}.
CC -!- DISRUPTION PHENOTYPE: Significantly increase of stride length and
CC extension movements in all limbs. Mice have major difficulties running
CC at higher velocities. In water, mice spend less time swimming and
CC showed frequent twitching limb movements.
CC {ECO:0000269|PubMed:22932389}.
CC -!- MISCELLANEOUS: DMRT3 is a marker for a subset of spinal cord neurons
CC (dI6).
CC -!- SIMILARITY: Belongs to the DMRT family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=A gait on the wildside
CC - Issue 154 of November 2013;
CC URL="https://web.expasy.org/spotlight/back_issues/154/";
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DR EMBL; AF541936; AAN77230.1; -; mRNA.
DR EMBL; BC052041; AAH52041.2; -; mRNA.
DR CCDS; CCDS29718.1; -.
DR RefSeq; NP_796334.2; NM_177360.3.
DR RefSeq; XP_006527129.1; XM_006527066.3.
DR AlphaFoldDB; Q80WT2; -.
DR SMR; Q80WT2; -.
DR STRING; 10090.ENSMUSP00000046812; -.
DR iPTMnet; Q80WT2; -.
DR PhosphoSitePlus; Q80WT2; -.
DR PaxDb; Q80WT2; -.
DR PRIDE; Q80WT2; -.
DR ProteomicsDB; 279388; -.
DR Antibodypedia; 23783; 164 antibodies from 23 providers.
DR DNASU; 240590; -.
DR Ensembl; ENSMUST00000048935; ENSMUSP00000046812; ENSMUSG00000042372.
DR GeneID; 240590; -.
DR KEGG; mmu:240590; -.
DR UCSC; uc008hbk.2; mouse.
DR CTD; 58524; -.
DR MGI; MGI:2449470; Dmrt3.
DR VEuPathDB; HostDB:ENSMUSG00000042372; -.
DR eggNOG; KOG3815; Eukaryota.
DR GeneTree; ENSGT00940000160420; -.
DR HOGENOM; CLU_052449_1_0_1; -.
DR InParanoid; Q80WT2; -.
DR OMA; RWASEPQ; -.
DR OrthoDB; 897429at2759; -.
DR PhylomeDB; Q80WT2; -.
DR TreeFam; TF317837; -.
DR BioGRID-ORCS; 240590; 5 hits in 73 CRISPR screens.
DR PRO; PR:Q80WT2; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q80WT2; protein.
DR Bgee; ENSMUSG00000042372; Expressed in lacrimal gland and 54 other tissues.
DR ExpressionAtlas; Q80WT2; baseline and differential.
DR Genevisible; Q80WT2; MM.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0007628; P:adult walking behavior; IMP:MGI.
DR GO; GO:0046661; P:male sex differentiation; IMP:MGI.
DR GO; GO:0042487; P:regulation of odontogenesis of dentin-containing tooth; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007548; P:sex differentiation; IBA:GO_Central.
DR GO; GO:0019226; P:transmission of nerve impulse; IMP:MGI.
DR GO; GO:0021521; P:ventral spinal cord interneuron specification; IMP:MGI.
DR Gene3D; 4.10.1040.10; -; 1.
DR InterPro; IPR001275; DM_DNA-bd.
DR InterPro; IPR036407; DM_DNA-bd_sf.
DR InterPro; IPR005173; DMA.
DR InterPro; IPR026607; DMRT.
DR InterPro; IPR009060; UBA-like_sf.
DR PANTHER; PTHR12322; PTHR12322; 1.
DR Pfam; PF00751; DM; 1.
DR Pfam; PF03474; DMA; 1.
DR SMART; SM00301; DM; 1.
DR SUPFAM; SSF46934; SSF46934; 1.
DR SUPFAM; SSF82927; SSF82927; 1.
DR PROSITE; PS40000; DM_1; 1.
DR PROSITE; PS50809; DM_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; DNA-binding; Metal-binding;
KW Nucleus; Reference proteome; Sexual differentiation; Transcription;
KW Transcription regulation; Zinc.
FT CHAIN 1..476
FT /note="Doublesex- and mab-3-related transcription factor 3"
FT /id="PRO_0000277795"
FT DOMAIN 255..290
FT /note="DMA"
FT /evidence="ECO:0000255"
FT DNA_BIND 29..76
FT /note="DM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00070"
FT REGION 89..130
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 147..195
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 418..476
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 418..436
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 93
FT /note="A -> D (in Ref. 1; AAN77230)"
FT /evidence="ECO:0000305"
FT CONFLICT 301
FT /note="A -> T (in Ref. 1; AAN77230)"
FT /evidence="ECO:0000305"
FT CONFLICT 361
FT /note="P -> H (in Ref. 1; AAN77230)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 476 AA; 51518 MW; 7FC6A4ED5DAE62A1 CRC64;
MNGYGSPYLY MGGPVSQPPR APLQRTPKCA RCRNHGVLSW LKGHKRYCRF KDCTCEKCIL
IIERQRVMAA QVALRRQQAN ESLESLIPDS LRALPGPPPP GDAAATAATA SQSSPASQAS
QPPAPPRPTA ELAAAAALRW VAEPQPGTLP AQLAKPDLTE ERVGDSSSTD NTAEAFSDKD
TDQRSSPDVV KSKNCFTPES PEIVSVDEGG YAVQKNGGNP ESCPDSPKYH AEQSHLLIEG
PSGTVSLPFS LKANRPPLEV LKKIFPNQKP TVLELILKGC GGDLVSAVEV LLSSRSSAAG
AERTAEESLV LPSSGHIFEH TLGSYPISSS KWSVGSAFRV PDTLRFSADS SNVVPNPLAV
PLQHPFPQPP RYPLMLRNTL ARNQSSPFLP NDVTLWNTMT LQQQYQLRSQ YVSPFPSNST
SVFRSSPVLS SRTTEDPRIS IPDDGCPIVT KQSIYTEDDY DERSDSSDSR ILNTSS
