ID   DMS1_PITNO              Reviewed;          73 AA.
AC   J7H8J4;
DT   05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2012, sequence version 1.
DT   25-MAY-2022, entry version 26.
DE   RecName: Full=Dermaseptin-1 {ECO:0000303|PubMed:24113627};
DE   Flags: Precursor;
OS   Pithecopus nordestinus (Northeastern Brazilian leaf frog) (Phyllomedusa
OS   nordestina).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC   Pithecopus.
OX   NCBI_TaxID=2034992 {ECO:0000312|EMBL:AFP89761.1};
RN   [1] {ECO:0000312|EMBL:AFP89761.1}
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Skin {ECO:0000303|PubMed:23159791};
RX   PubMed=23159791; DOI=10.1016/j.toxicon.2012.10.016;
RA   Neiva M., Vargas D.C., Conceicao K., Radis-Baptista G., Assakura M.T.,
RA   Jared C., Hayashi M.A.;
RT   "Gene expression analysis by ESTs sequencing of the Brazilian frog
RT   Phyllomedusa nordestina skin glands.";
RL   Toxicon 61:139-150(2013).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 46-70, FUNCTION, SUBCELLULAR LOCATION, MASS
RP   SPECTROMETRY, AND AMIDATION AT LEU-70.
RC   TISSUE=Skin secretion {ECO:0000303|PubMed:24113627};
RX   PubMed=24113627; DOI=10.1016/j.exppara.2013.09.016;
RA   Pinto E.G., Pimenta D.C., Antoniazzi M.M., Jared C., Tempone A.G.;
RT   "Antimicrobial peptides isolated from Phyllomedusa nordestina (Amphibia)
RT   alter the permeability of plasma membrane of Leishmania and Trypanosoma
RT   cruzi.";
RL   Exp. Parasitol. 135:655-660(2013).
CC   -!- FUNCTION: Has antiparasitic activity against trypomastigote form of
CC       T.cruzi (IC(50)=0.68 uM) in vitro but not against L.infantum
CC       (PubMed:24113627). Probably acts by permeabilizing cell membranes
CC       (PubMed:24113627). In vitro, shows no cytotoxicity against macrophages
CC       (PubMed:24113627). Has antibacterial activity (By similarity).
CC       {ECO:0000250|UniProtKB:Q1EJP5, ECO:0000269|PubMed:24113627}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255,
CC       ECO:0000269|PubMed:24113627}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:23159791}.
CC   -!- MASS SPECTROMETRY: Mass=2410.88; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:24113627};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Dermaseptin subfamily. {ECO:0000305}.
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DR   EMBL; JX127159; AFP89761.1; -; mRNA.
DR   AlphaFoldDB; J7H8J4; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR022731; Dermaseptin.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   InterPro; IPR016322; FSAP.
DR   Pfam; PF12121; DD_K; 1.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
DR   PIRSF; PIRSF001822; Dermaseptin_precursor; 1.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Cleavage on pair of basic residues; Direct protein sequencing; Secreted;
KW   Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..43
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000305|PubMed:24113627"
FT                   /id="PRO_0000441004"
FT   PEPTIDE         46..70
FT                   /note="Dermaseptin-1"
FT                   /evidence="ECO:0000269|PubMed:24113627"
FT                   /id="PRO_0000441005"
FT   PROPEP          72..73
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000305|PubMed:24113627"
FT                   /id="PRO_0000441006"
FT   REGION          25..46
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         70
FT                   /note="Leucine amide"
FT                   /evidence="ECO:0000269|PubMed:24113627"
SQ   SEQUENCE   73 AA;  8005 MW;  8A33590B26E8B137 CRC64;
     MAFLKKSIFL ALFLGMVSLS ICEEEKRENE GEEEQEDDEQ SEMKRGLWST IKNVGKEAAI
     AAGKAALGAL GEQ