DMT3A_DANRE
ID DMT3A_DANRE Reviewed; 448 AA.
AC P83758; A2BFC1; Q4VUS9; Q5XJM6; Q694V7; Q9DD75;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Doublesex- and mab-3-related transcription factor 3a;
DE AltName: Full=Dmrt7a;
GN Name=dmrt3a; ORFNames=si:dkeyp-31e5.2, zgc:101766;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 4-238.
RA Li Q., Zhou R.;
RT "Genomic structure and expression of the zebrafish DMRT3 gene.";
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Olfactory epithelium;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 36-66.
RC TISSUE=Embryo {ECO:0000269|PubMed:11932949};
RX PubMed=11932949; DOI=10.1002/jez.10044;
RA Huang X., Cheng H., Guo Y., Liu L., Gui J., Zhou R.;
RT "A conserved family of doublesex-related genes from fishes.";
RL J. Exp. Zool. 294:63-67(2002).
RN [5]
RP SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, TISSUE EXPRESSION, AND
RP MUTAGENESIS OF 41-LYS--ARG-45.
RX PubMed=18282142; DOI=10.1042/bc20070114;
RA Li Q., Zhou X., Guo Y., Shang X., Chen H., Lu H., Cheng H., Zhou R.;
RT "Nuclear localization, DNA binding and restricted expression in neural and
RT germ cells of zebrafish Dmrt3.";
RL Biol. Cell 100:453-463(2008).
CC -!- FUNCTION: Probable transcription factor that plays a role in
CC configuring the spinal circuits controlling motion in vertebrates.
CC Involved in neuronal specification within specific subdivision of
CC spinal cord neurons and in the development of a coordinated locomotor
CC network controlling movements. May regulate transcription (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00070,
CC ECO:0000269|PubMed:18282142}.
CC -!- TISSUE SPECIFICITY: Expressed in the developing germ cells of both
CC gonads, mainly in spermatogonia, spermatocytes and developing oocytes.
CC Also expressed in embryonic olfactory placode and neural tube.
CC -!- DEVELOPMENTAL STAGE: Expression increases gradually to a high level at
CC pharyngula stage, which is restricted to the olfactory placode and the
CC neural tube. In the juvenile zebrafish, the expression is first
CC detected in undifferentiated gonad on 17 dpf.
CC {ECO:0000269|PubMed:18282142}.
CC -!- SIMILARITY: Belongs to the DMRT family. {ECO:0000305}.
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DR EMBL; AY621083; AAU89440.1; -; mRNA.
DR EMBL; AY621084; AAT88067.1; -; Genomic_DNA.
DR EMBL; BC083273; AAH83273.1; -; mRNA.
DR EMBL; BX255951; CAM16634.1; -; Genomic_DNA.
DR EMBL; AF272964; AAG18560.1; -; Genomic_DNA.
DR RefSeq; NP_001005779.2; NM_001005779.2.
DR AlphaFoldDB; P83758; -.
DR SMR; P83758; -.
DR STRING; 7955.ENSDARP00000051145; -.
DR PaxDb; P83758; -.
DR Ensembl; ENSDART00000051146; ENSDARP00000051145; ENSDARG00000035290.
DR Ensembl; ENSDART00000191412; ENSDARP00000145328; ENSDARG00000114587.
DR GeneID; 450035; -.
DR KEGG; dre:450035; -.
DR CTD; 450035; -.
DR ZFIN; ZDB-GENE-021220-3; dmrt3a.
DR eggNOG; KOG3815; Eukaryota.
DR GeneTree; ENSGT00940000160420; -.
DR HOGENOM; CLU_052449_1_0_1; -.
DR InParanoid; P83758; -.
DR OMA; GPFMHND; -.
DR OrthoDB; 897429at2759; -.
DR PhylomeDB; P83758; -.
DR TreeFam; TF317837; -.
DR PRO; PR:P83758; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 5.
DR Bgee; ENSDARG00000035290; Expressed in pharyngeal gill and 5 other tissues.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:ZFIN.
DR GO; GO:0046661; P:male sex differentiation; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007548; P:sex differentiation; IBA:GO_Central.
DR Gene3D; 4.10.1040.10; -; 1.
DR InterPro; IPR001275; DM_DNA-bd.
DR InterPro; IPR036407; DM_DNA-bd_sf.
DR InterPro; IPR005173; DMA.
DR InterPro; IPR026607; DMRT.
DR InterPro; IPR009060; UBA-like_sf.
DR PANTHER; PTHR12322; PTHR12322; 1.
DR Pfam; PF00751; DM; 1.
DR Pfam; PF03474; DMA; 1.
DR SMART; SM00301; DM; 1.
DR SUPFAM; SSF46934; SSF46934; 1.
DR SUPFAM; SSF82927; SSF82927; 1.
DR PROSITE; PS40000; DM_1; 1.
DR PROSITE; PS50809; DM_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc.
FT CHAIN 1..448
FT /note="Doublesex- and mab-3-related transcription factor
FT 3a"
FT /id="PRO_0000207049"
FT DOMAIN 223..258
FT /note="DMA"
FT /evidence="ECO:0000255"
FT DNA_BIND 28..75
FT /note="DM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00070"
FT REGION 98..203
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 424..448
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 98..141
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 158..181
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 41..45
FT /note="KGHKR->AAAAA: Inhibits the translocation of Dmrt3
FT into the nucleus."
FT /evidence="ECO:0000269|PubMed:18282142"
FT CONFLICT 117
FT /note="N -> S (in Ref. 1; AAU89440/AAT88067)"
FT /evidence="ECO:0000305"
FT CONFLICT 284
FT /note="N -> S (in Ref. 3; AAH83273)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 448 AA; 48965 MW; 21E03094D9685464 CRC64;
MNGYGSPYLY MGGPVSQPRA PLQRTPKCAR CRNHGVLSWL KGHKRYCRFK DCTCEKCILI
IERQRVMAAQ VALRRQQANE SLESLIPESL RALPGITVSS GEQSANPRAS DIDLRWNTET
GTPKTPQDLS DELSGEQSGG ENGGSDREPE AVSSPEESKP NLCCTPDTSD PSPQLEESRF
GLSKSCSDPE PKSDNPKYSP EPNLLIEGLS GSVSLPFSLR ANRPPLEVLK KIFPAHKPAV
LELILKGCGG DLVGAIEILL SSRSTMKPEK ILSESSDALV LPSNGHLFEH PLSSYPVSSS
KWSVGSAFRV PDSLRFSSES SGGVVPGPLS MPLQHGFPQP PRYPLMLRNS LSRGQAGPFM
HNDVTLWNTM TLQQQYQLRS QYVPPFSGPS AGVIRSSPLL TSRPSEEHRI SIQEESCPVI
AKQSMYAADE EYDDRSDSSD SRILNTSS
