ADDA_CALS4
ID ADDA_CALS4 Reviewed; 1204 AA.
AC Q8RCZ0;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=ATP-dependent helicase/nuclease subunit A {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01451};
DE AltName: Full=ATP-dependent helicase/nuclease AddA {ECO:0000255|HAMAP-Rule:MF_01451};
GN Name=addA {ECO:0000255|HAMAP-Rule:MF_01451}; OrderedLocusNames=TTE0264;
OS Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM
OS 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis).
OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacteraceae; Caldanaerobacter.
OX NCBI_TaxID=273068;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15242 / JCM 11007 / NBRC 100824 / MB4;
RX PubMed=11997336; DOI=10.1101/gr.219302;
RA Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y.,
RA Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R.,
RA Wang J., Yu J., Yang H.;
RT "A complete sequence of the T. tengcongensis genome.";
RL Genome Res. 12:689-700(2002).
CC -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC and an ATP-dependent, dual-direction single-stranded exonuclease.
CC Recognizes the chi site generating a DNA molecule suitable for the
CC initiation of homologous recombination. The AddA nuclease domain is
CC required for chi fragment generation; this subunit has the helicase and
CC 3' -> 5' nuclease activities. {ECO:0000255|HAMAP-Rule:MF_01451}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- SUBUNIT: Heterodimer of AddA and AddB/RexB. {ECO:0000255|HAMAP-
CC Rule:MF_01451}.
CC -!- SIMILARITY: Belongs to the helicase family. AddA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01451}.
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DR EMBL; AE008691; AAM23560.1; -; Genomic_DNA.
DR RefSeq; WP_011024733.1; NC_003869.1.
DR AlphaFoldDB; Q8RCZ0; -.
DR SMR; Q8RCZ0; -.
DR STRING; 273068.TTE0264; -.
DR EnsemblBacteria; AAM23560; AAM23560; TTE0264.
DR KEGG; tte:TTE0264; -.
DR eggNOG; COG1074; Bacteria.
DR HOGENOM; CLU_001114_3_1_9; -.
DR OMA; KQSIYRW; -.
DR OrthoDB; 137860at2; -.
DR Proteomes; UP000000555; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 4.
DR Gene3D; 3.90.320.10; -; 1.
DR HAMAP; MF_01451; AddA; 1.
DR InterPro; IPR014152; DNA_helicase_suAddA.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR PANTHER; PTHR11070:SF48; PTHR11070:SF48; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
DR TIGRFAMs; TIGR02785; addA_Gpos; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Exonuclease; Helicase;
KW Hydrolase; Nuclease; Nucleotide-binding; Reference proteome.
FT CHAIN 1..1204
FT /note="ATP-dependent helicase/nuclease subunit A"
FT /id="PRO_0000379362"
FT DOMAIN 3..460
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT DOMAIN 492..780
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT BINDING 24..31
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
SQ SEQUENCE 1204 AA; 141346 MW; 36AED0B3EB5CDBAF CRC64;
MEVKWTEEQR EAITTRGKNV LVAAAAGSGK TAVLVERIIN IVTDPEKPVD IDRLLVVTFT
NAAASEMRER IAERLISLLD QHPEDKRLAD QLTLLNKATI TTIHSFCLDV VRKHFFLLDL
DPSFRVGDDT ETLLLKLEAM EELFEELYEK NDEGFLLLVE SYGGTKGDQY LQDVLLKLYG
FIRSLPWPEK WLNNVLEAFE VKEDFSFEDS KWAEVILDSI KVEILGLLNG MSVAIEKLRE
ERGLEGYLSL FQSEAHHLKE LIDSKSWNEF KKKIEAISFE RLPKASKEAD AEVKEEVRKL
REDVKKRIRE IRDKFFIDEE EEIKREIKKL YPVMKALADL ILMFDKKYGE KKKEKGVIDF
EDIEHFALKI LSNEEVVSYY REKFEEIFID EYQDSSLIQE AILSLIARDN PPNRFMVGDV
KQSIYRFRQA NPYIFFEKYT SYSQEGENKK ILLYKNFRSR KEIIDAVNYI FEKIMSKNIG
EVDYSEEEKL NYGANYESNP FEKENERTVE VHLIEREREE EFEEDEEILD DMQVEAHVVG
ERIKGLFREG FKVYDKEIGG YRPVEYRDIC VLLRATEKWA YAFEEEFVKM GIPVFADTNA
GYFDTAEIKT MLSLLQVIDN PMQDIPLLAV LRSPIFSFTE EELVDIRLED ADGTIYEALK
KASLREDELG EKVRNFLDSL KRWQEKSIYM PVDEFLWYLY EETGFYSYVG AMPQGVERQA
NLRVLFERAK EYEETSFKGL FNFVNFINRL RTTSTDMGSA KTVGENENVV RVMSIHKSKG
LEFPIVIVAG LGKQFNTKDL NEKILYHHFL GIGPEFVDYR RRLSYPSIVK EAIKYKIKLE
SLSEEMRVLY VALTRAKEKL ILVASVKDIK ERAKKWGKAK LLGKKISEYD VLKSRSYIDW
IGSALIRHRD LKLLREFAEI SPELEQDSSK WEVKIWNKRD VIIERKKDEG IEVLERLNAL
NLEGLHTEFR KEVEERLNYV YPYDRSSKLP AKLSVTEIKR ILNDEVIDEE TTSIFERRVL
KTPLFLEKKR GLTPAERGIA MHLVMQKLDL SKDLSYRGIK EQIKDMVKNE ILTEEQAKEV
DANKIERFFK TPLGKRLLKA KEVRREVPFH IKISSREIYR DLPEVYQEEF IAVQGIIDCF
FEEEGELVLI DYKTDYVENG KIEEIRDKYR VQIDLYGKAL EEITGKKVKE KYIYLFFNDT
IIKY
