DN7E_SULAC
ID DN7E_SULAC Reviewed; 65 AA.
AC P13125; Q4JBQ1;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=DNA-binding protein 7e {ECO:0000305};
DE AltName: Full=7 kDa DNA-binding protein e;
DE AltName: Full=Sac7e;
GN OrderedLocusNames=Saci_0362;
OS Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC
OS 15157 / NCIMB 11770).
OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Sulfolobus.
OX NCBI_TaxID=330779;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DNA-BINDING.
RX PubMed=7632679; DOI=10.1021/bi00031a031;
RA McAfee J.G., Edmondson S.P., Datta P.K., Shriver J.W., Gupta R.;
RT "Gene cloning, expression, and characterization of the Sac7 proteins from
RT the hyperthermophile Sulfolobus acidocaldarius.";
RL Biochemistry 34:10063-10077(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RX PubMed=15995215; DOI=10.1128/jb.187.14.4992-4999.2005;
RA Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E.,
RA Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.;
RT "The genome of Sulfolobus acidocaldarius, a model organism of the
RT Crenarchaeota.";
RL J. Bacteriol. 187:4992-4999(2005).
RN [3]
RP PROTEIN SEQUENCE OF 2-65, AND METHYLATION AT LYS-7; LYS-63 AND LYS-64.
RX PubMed=3130377; DOI=10.1016/s0021-9258(18)68608-9;
RA Choli T., Wittmann-Liebold B., Reinhardt R.;
RT "Microsequence analysis of DNA-binding proteins 7a, 7b, and 7e from the
RT archaebacterium Sulfolobus acidocaldarius.";
RL J. Biol. Chem. 263:7087-7093(1988).
RN [4]
RP DNA-BINDING, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, AND SUBCELLULAR
RP LOCATION.
RX PubMed=27853299; DOI=10.1038/srep37274;
RA Kalichuk V., Behar G., Renodon-Corniere A., Danovski G., Obal G.,
RA Barbet J., Mouratou B., Pecorari F.;
RT "The archaeal '7 kDa DNA-binding' proteins: extended characterization of an
RT old gifted family.";
RL Sci. Rep. 6:37274-37274(2016).
CC -!- FUNCTION: Can constrain negative DNA supercoils. May be involved in
CC maintaining the integrity of the genome at high temperature.
CC {ECO:0000250|UniProtKB:P61990}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Highly stable from pH 0 to pH 12. {ECO:0000269|PubMed:27853299};
CC Temperature dependence:
CC Hyperthermostable. {ECO:0000269|PubMed:27853299};
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:27853299}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:27853299}.
CC -!- PTM: Lys-63 was found to be 25% monomethylated and Lys-64 was found to
CC be 36% monomethylated. {ECO:0000269|PubMed:3130377}.
CC -!- MISCELLANEOUS: 7e possesses the tighter binding as compared to that of
CC the proteins 7a and 7b, which may be due to the three supplementary
CC lysines in the C-terminal region. {ECO:0000269|PubMed:3130377}.
CC -!- SIMILARITY: Belongs to the 7 kDa DNA-binding/endoribonuclease P2
CC family. {ECO:0000305}.
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DR EMBL; L08891; AAA80316.1; -; Genomic_DNA.
DR EMBL; CP000077; AAY79778.1; -; Genomic_DNA.
DR PIR; C27749; C27749.
DR RefSeq; WP_011277280.1; NC_007181.1.
DR AlphaFoldDB; P13125; -.
DR SMR; P13125; -.
DR STRING; 330779.Saci_0362; -.
DR iPTMnet; P13125; -.
DR EnsemblBacteria; AAY79778; AAY79778; Saci_0362.
DR GeneID; 3473140; -.
DR KEGG; sai:Saci_0362; -.
DR PATRIC; fig|330779.12.peg.359; -.
DR eggNOG; arCOG05888; Archaea.
DR HOGENOM; CLU_2929990_0_0_2; -.
DR OMA; PKELMDM; -.
DR Proteomes; UP000001018; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004521; F:endoribonuclease activity; IEA:InterPro.
DR InterPro; IPR016197; Chromo-like_dom_sf.
DR InterPro; IPR003212; DNA-bd_7kDa_arc.
DR Pfam; PF02294; 7kD_DNA_binding; 1.
DR PIRSF; PIRSF036912; Sac7; 1.
DR SUPFAM; SSF54160; SSF54160; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Direct protein sequencing; DNA-binding; Methylation;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:3130377"
FT CHAIN 2..65
FT /note="DNA-binding protein 7e"
FT /id="PRO_0000213075"
FT MOD_RES 7
FT /note="N6-methyllysine"
FT /evidence="ECO:0000269|PubMed:3130377"
FT MOD_RES 63
FT /note="N6-methyllysine; partial"
FT /evidence="ECO:0000269|PubMed:3130377"
FT MOD_RES 64
FT /note="N6-methyllysine; partial"
FT /evidence="ECO:0000269|PubMed:3130377"
SQ SEQUENCE 65 AA; 7470 MW; B0672CF624C39789 CRC64;
MAKVRFKYKG EEKEVDTSKI KKVWRVGKMV SFTYDDNGKT GRGAVSEKDA PKELMDMLAR
AEKKK
