ADDA_CARHZ
ID ADDA_CARHZ Reviewed; 1167 AA.
AC Q3AA35;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=ATP-dependent helicase/nuclease subunit A {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01451};
DE AltName: Full=ATP-dependent helicase/nuclease AddA {ECO:0000255|HAMAP-Rule:MF_01451};
GN Name=addA {ECO:0000255|HAMAP-Rule:MF_01451}; Synonyms=rexA;
GN OrderedLocusNames=CHY_2186;
OS Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 /
OS Z-2901).
OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacteraceae; Carboxydothermus.
OX NCBI_TaxID=246194;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-161 / DSM 6008 / Z-2901;
RX PubMed=16311624; DOI=10.1371/journal.pgen.0010065;
RA Wu M., Ren Q., Durkin A.S., Daugherty S.C., Brinkac L.M., Dodson R.J.,
RA Madupu R., Sullivan S.A., Kolonay J.F., Nelson W.C., Tallon L.J.,
RA Jones K.M., Ulrich L.E., Gonzalez J.M., Zhulin I.B., Robb F.T., Eisen J.A.;
RT "Life in hot carbon monoxide: the complete genome sequence of
RT Carboxydothermus hydrogenoformans Z-2901.";
RL PLoS Genet. 1:563-574(2005).
CC -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC and an ATP-dependent, dual-direction single-stranded exonuclease.
CC Recognizes the chi site generating a DNA molecule suitable for the
CC initiation of homologous recombination. The AddA nuclease domain is
CC required for chi fragment generation; this subunit has the helicase and
CC 3' -> 5' nuclease activities. {ECO:0000255|HAMAP-Rule:MF_01451}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- SUBUNIT: Heterodimer of AddA and AddB/RexB. {ECO:0000255|HAMAP-
CC Rule:MF_01451}.
CC -!- SIMILARITY: Belongs to the helicase family. AddA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01451}.
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DR EMBL; CP000141; ABB14602.1; -; Genomic_DNA.
DR RefSeq; WP_011345072.1; NC_007503.1.
DR AlphaFoldDB; Q3AA35; -.
DR SMR; Q3AA35; -.
DR STRING; 246194.CHY_2186; -.
DR EnsemblBacteria; ABB14602; ABB14602; CHY_2186.
DR KEGG; chy:CHY_2186; -.
DR eggNOG; COG1074; Bacteria.
DR HOGENOM; CLU_001114_3_1_9; -.
DR OMA; KQSIYRW; -.
DR OrthoDB; 137860at2; -.
DR Proteomes; UP000002706; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 4.
DR Gene3D; 3.90.320.10; -; 1.
DR HAMAP; MF_01451; AddA; 1.
DR InterPro; IPR014152; DNA_helicase_suAddA.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR PANTHER; PTHR11070:SF48; PTHR11070:SF48; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
DR TIGRFAMs; TIGR02785; addA_Gpos; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Exonuclease; Helicase;
KW Hydrolase; Nuclease; Nucleotide-binding; Reference proteome.
FT CHAIN 1..1167
FT /note="ATP-dependent helicase/nuclease subunit A"
FT /id="PRO_0000379246"
FT DOMAIN 2..451
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT DOMAIN 478..768
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT BINDING 23..30
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
SQ SEQUENCE 1167 AA; 134105 MW; 35773DC0BC7578F9 CRC64;
MKNWTAEQMR AITSRGNALL VSAAAGAGKT SVLVERVLSR VLTDTPPVDI DRLLVVTFTE
AAAGEMKERL GTELLKRLNE DPGNSRILEQ LELLPVADIS TLHSFCHKII RKYGRVCGYE
TKFAILEGPR ETYLKNKVLE EILEERYEKG DRELFALLEY LNDEKNDRNL KELILNLYHF
SRSNPEPEKW LLDSLNLFNG NWERFEDTFW YREIKSSTEM WLEYILELLG RAKSVAEKYG
QARAFSLLAE DMEKVRGLYA KLNEGYEAAK QYLSQVKFGT FSWGRGVGGK DEAKDLRDKA
KEQFETIKKR YFSWEAGNFR EELRTLTSYL DPLVKLVREF SRKYQEEKRK HGFADFSDLE
HWALDILKSG VYRELREKYV EILVDEYQDI NGLQEEILTY VSRDGQNLFM VGDVKQSIYR
FRWARPEIFL KKYEDFTDEK KIELSLNFRS REEIIATVNF IFKQIMKKRV AELSYDEKAF
LKKGADYLPN ANCFAELHLI EGKPEEDINS NGEPEEDLTA VHREARLVAA KILKLKEEGF
KVFDRETKEF RPLQYRDIVI LSRSLSNSSN IWQEELTRAG IPVYVEGAGS FLNSKEILLM
TSFLKVIDNP CQDVPLAAVL CSPVAGLTYE ELWQVRKEYP EGLLYDALKN KSLGKDELSV
KSQKFLELLV EFQKLSRQIS LAELVNEIYR KTNLPEIFGA YPGGEVRQAN LKLLHDLAVD
FAEINGGGIY NFLTFLSQAA ESEDFSPAKL IGEADDVVRV MSVHKSKGLE FPVVFVVGLG
KRFKFDYSNT VFLHSDLGFG PKFFDPEKRI RRHSIASQIL SERMRRETLA EEMRILYVAL
TRAREKLILV GTVKNLAKKM AGWQSQTEAL KDTLSDGQIA RAGNFLDWIG PVVFREGSDL
PDCLKVEVHP QQEKIEGEQW ELPEVLRVKL LTKTPFTEET DYTGQFRAGL EFNYPGLKIA
KLPAKMSVSD LKEVFSTDDV ISLEDEDEVF LPGVYFEDGA MLGIVYHEFL RRIDFQGDLS
ASGLKAQGET LVAEGVLPPE SREMLDFTKI ARIFATPLGQ RILRAREIYP EFPFTLGVKA
GEIYPEATGF SEKILVRGVI DLLALEEDGF FIVDWKTDRV TGDILNERLK EYAGQLNLYA
RAVEEITGKK VKEKYLYFIN LEKEVRV
