DNAA_AQUAE
ID DNAA_AQUAE Reviewed; 399 AA.
AC O66659;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Chromosomal replication initiator protein DnaA;
GN Name=dnaA; OrderedLocusNames=aq_322;
OS Aquifex aeolicus (strain VF5).
OC Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX NCBI_TaxID=224324;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VF5;
RX PubMed=9537320; DOI=10.1038/32831;
RA Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL Nature 392:353-358(1998).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 76-399.
RX PubMed=12234917; DOI=10.1093/emboj/cdf496;
RA Erzberger J.P., Pirruccello M.M., Berger J.M.;
RT "The structure of bacterial DnaA: implications for general mechanisms
RT underlying DNA replication initiation.";
RL EMBO J. 21:4763-4773(2002).
CC -!- FUNCTION: Plays an important role in the initiation and regulation of
CC chromosomal replication. Binds to the origin of replication; it binds
CC specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-
CC TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids (By
CC similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC O66659; O66659: dnaA; NbExp=3; IntAct=EBI-15590648, EBI-15590648;
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the DnaA family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE000657; AAC06612.1; -; Genomic_DNA.
DR PIR; G70328; G70328.
DR RefSeq; NP_213219.1; NC_000918.1.
DR RefSeq; WP_010880157.1; NC_000918.1.
DR PDB; 1L8Q; X-ray; 2.70 A; A=76-399.
DR PDB; 2HCB; X-ray; 3.51 A; A/B/C/D=77-399.
DR PDB; 3R8F; X-ray; 3.37 A; A/B/C/D=76-399.
DR PDBsum; 1L8Q; -.
DR PDBsum; 2HCB; -.
DR PDBsum; 3R8F; -.
DR AlphaFoldDB; O66659; -.
DR SMR; O66659; -.
DR DIP; DIP-29219N; -.
DR STRING; 224324.aq_322; -.
DR EnsemblBacteria; AAC06612; AAC06612; aq_322.
DR KEGG; aae:aq_322; -.
DR PATRIC; fig|224324.8.peg.259; -.
DR eggNOG; COG0593; Bacteria.
DR HOGENOM; CLU_026910_3_2_0; -.
DR InParanoid; O66659; -.
DR OMA; HINFICK; -.
DR OrthoDB; 219876at2; -.
DR EvolutionaryTrace; O66659; -.
DR Proteomes; UP000000798; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR GO; GO:0006275; P:regulation of DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd06571; Bac_DnaA_C; 1.
DR Gene3D; 1.10.1750.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00377; DnaA_bact; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR001957; Chromosome_initiator_DnaA.
DR InterPro; IPR020591; Chromosome_initiator_DnaA-like.
DR InterPro; IPR018312; Chromosome_initiator_DnaA_CS.
DR InterPro; IPR013317; DnaA.
DR InterPro; IPR013159; DnaA_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR010921; Trp_repressor/repl_initiator.
DR PANTHER; PTHR30050:SF2; PTHR30050:SF2; 1.
DR Pfam; PF00308; Bac_DnaA; 1.
DR Pfam; PF08299; Bac_DnaA_C; 1.
DR PRINTS; PR00051; DNAA.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00760; Bac_DnaA_C; 1.
DR SUPFAM; SSF48295; SSF48295; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00362; DnaA; 1.
DR PROSITE; PS01008; DNAA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cytoplasm; DNA replication; DNA-binding;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..399
FT /note="Chromosomal replication initiator protein DnaA"
FT /id="PRO_0000114122"
FT BINDING 119..126
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT STRAND 85..87
FT /evidence="ECO:0007829|PDB:1L8Q"
FT TURN 92..94
FT /evidence="ECO:0007829|PDB:1L8Q"
FT HELIX 95..106
FT /evidence="ECO:0007829|PDB:1L8Q"
FT TURN 107..110
FT /evidence="ECO:0007829|PDB:1L8Q"
FT STRAND 113..118
FT /evidence="ECO:0007829|PDB:1L8Q"
FT STRAND 120..124
FT /evidence="ECO:0007829|PDB:1L8Q"
FT HELIX 125..138
FT /evidence="ECO:0007829|PDB:1L8Q"
FT STRAND 143..147
FT /evidence="ECO:0007829|PDB:1L8Q"
FT HELIX 148..161
FT /evidence="ECO:0007829|PDB:1L8Q"
FT HELIX 164..172
FT /evidence="ECO:0007829|PDB:1L8Q"
FT STRAND 175..180
FT /evidence="ECO:0007829|PDB:1L8Q"
FT HELIX 182..185
FT /evidence="ECO:0007829|PDB:1L8Q"
FT HELIX 189..204
FT /evidence="ECO:0007829|PDB:1L8Q"
FT STRAND 208..215
FT /evidence="ECO:0007829|PDB:1L8Q"
FT HELIX 217..219
FT /evidence="ECO:0007829|PDB:1L8Q"
FT HELIX 225..232
FT /evidence="ECO:0007829|PDB:1L8Q"
FT STRAND 234..238
FT /evidence="ECO:0007829|PDB:1L8Q"
FT HELIX 243..256
FT /evidence="ECO:0007829|PDB:1L8Q"
FT HELIX 263..272
FT /evidence="ECO:0007829|PDB:1L8Q"
FT HELIX 276..289
FT /evidence="ECO:0007829|PDB:1L8Q"
FT HELIX 291..316
FT /evidence="ECO:0007829|PDB:1L8Q"
FT HELIX 320..323
FT /evidence="ECO:0007829|PDB:1L8Q"
FT STRAND 330..332
FT /evidence="ECO:0007829|PDB:1L8Q"
FT HELIX 333..347
FT /evidence="ECO:0007829|PDB:1L8Q"
FT HELIX 351..357
FT /evidence="ECO:0007829|PDB:1L8Q"
FT HELIX 365..375
FT /evidence="ECO:0007829|PDB:1L8Q"
FT HELIX 383..398
FT /evidence="ECO:0007829|PDB:1L8Q"
SQ SEQUENCE 399 AA; 46840 MW; C641CB199AA79906 CRC64;
MELNALIKKI ESVDSYAREF LKKFEIKQEK GKFLFIAPGE DYREWLETIV NTFLEEEVRK
LIEVKEKEEK KKVEIKDFLN PKYTLENFIV GEGNRLAYEV VKEALENLGS LYNPIFIYGS
VGTGKTHLLQ AAGNEAKKRG YRVIYSSADD FAQAMVEHLK KGTINEFRNM YKSVDLLLLD
DVQFLSGKER TQIEFFHIFN TLYLLEKQII LASDRHPQKL DGVSDRLVSR FEGGILVEIE
LDNKTRFKII KEKLKEFNLE LRKEVIDYLL ENTKNVREIE GKIKLIKLKG FEGLERKERK
ERDKLMQIVE FVANYYAVKV EDILSDKRNK RTSEARKIAM YLCRKVCSAS LIEIARAFKR
KDHTTVIHAI RSVEEEKKKD RKFKHLVGFL EKQAFDKIC
