ID   DNAA_BACSU              Reviewed;         446 AA.
AC   P05648;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   03-AUG-2022, entry version 160.
DE   RecName: Full=Chromosomal replication initiator protein DnaA;
GN   Name=dnaA; Synonyms=dnaH; OrderedLocusNames=BSU00010;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2987847; DOI=10.1093/nar/13.7.2251;
RA   Moriya S., Ogasawara N., Yoshikawa H.;
RT   "Structure and function of the region of the replication origin of the
RT   Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base
RT   pairs in the origin region.";
RL   Nucleic Acids Res. 13:2251-2265(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=7584024; DOI=10.1093/dnares/1.1.1;
RA   Ogasawara N., Nakai S., Yoshikawa H.;
RT   "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis
RT   chromosome containing the replication origin.";
RL   DNA Res. 1:1-14(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6 AND 441-446.
RC   STRAIN=168 / PSL1;
RX   PubMed=2846289; DOI=10.1002/j.1460-2075.1988.tb03149.x;
RA   Moriya S., Fukuoka T., Ogasawara N., Yoshikawa H.;
RT   "Regulation of initiation of the chromosomal replication by DnaA-boxes in
RT   the origin region of the Bacillus subtilis chromosome.";
RL   EMBO J. 7:2911-2917(1988).
CC   -!- FUNCTION: Plays an important role in the initiation and regulation of
CC       chromosomal replication. Binds to the origin of replication; it binds
CC       specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-
CC       TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids. DnaA
CC       can inhibit its own gene expression as well as that of other genes.
CC   -!- SUBUNIT: Some 20 DnaA protein molecules bind their sites in oriC.
CC   -!- INTERACTION:
CC       P05648; P05648: dnaA; NbExp=2; IntAct=EBI-2014722, EBI-2014722;
CC       P05648; P37522: soj; NbExp=2; IntAct=EBI-2014722, EBI-2014674;
CC       P05648; P37542: yabA; NbExp=4; IntAct=EBI-2014722, EBI-5243764;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DnaA family. {ECO:0000305}.
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DR   EMBL; X02369; CAA26217.1; -; Genomic_DNA.
DR   EMBL; D26185; BAA05237.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB11777.1; -; Genomic_DNA.
DR   EMBL; X12778; CAA31269.1; -; Genomic_DNA.
DR   EMBL; X12779; CAA31270.1; -; Genomic_DNA.
DR   PIR; A22930; IQBSOC.
DR   RefSeq; NP_387882.1; NC_000964.3.
DR   RefSeq; WP_003242674.1; NZ_JNCM01000034.1.
DR   PDB; 4TPS; X-ray; 1.65 A; B/D=1-82.
DR   PDBsum; 4TPS; -.
DR   AlphaFoldDB; P05648; -.
DR   SMR; P05648; -.
DR   DIP; DIP-41940N; -.
DR   IntAct; P05648; 7.
DR   STRING; 224308.BSU00010; -.
DR   PaxDb; P05648; -.
DR   PRIDE; P05648; -.
DR   EnsemblBacteria; CAB11777; CAB11777; BSU_00010.
DR   GeneID; 50135287; -.
DR   GeneID; 939978; -.
DR   KEGG; bsu:BSU00010; -.
DR   PATRIC; fig|224308.179.peg.1; -.
DR   eggNOG; COG0593; Bacteria.
DR   InParanoid; P05648; -.
DR   OMA; REFNPLF; -.
DR   PhylomeDB; P05648; -.
DR   BioCyc; BSUB:BSU00010-MON; -.
DR   PRO; PR:P05648; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR   GO; GO:0006270; P:DNA replication initiation; IDA:CACAO.
DR   GO; GO:0006275; P:regulation of DNA replication; IEA:UniProtKB-UniRule.
DR   CDD; cd06571; Bac_DnaA_C; 1.
DR   Gene3D; 1.10.1750.10; -; 1.
DR   Gene3D; 3.30.300.180; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00377; DnaA_bact; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR001957; Chromosome_initiator_DnaA.
DR   InterPro; IPR020591; Chromosome_initiator_DnaA-like.
DR   InterPro; IPR018312; Chromosome_initiator_DnaA_CS.
DR   InterPro; IPR013317; DnaA.
DR   InterPro; IPR013159; DnaA_C.
DR   InterPro; IPR024633; DnaA_N_dom.
DR   InterPro; IPR038454; DnaA_N_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR010921; Trp_repressor/repl_initiator.
DR   PANTHER; PTHR30050:SF2; PTHR30050:SF2; 1.
DR   Pfam; PF00308; Bac_DnaA; 1.
DR   Pfam; PF08299; Bac_DnaA_C; 1.
DR   Pfam; PF11638; DnaA_N; 1.
DR   PRINTS; PR00051; DNAA.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00760; Bac_DnaA_C; 1.
DR   SUPFAM; SSF48295; SSF48295; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00362; DnaA; 1.
DR   PROSITE; PS01008; DNAA; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; Cytoplasm; DNA replication; DNA-binding;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..446
FT                   /note="Chromosomal replication initiator protein DnaA"
FT                   /id="PRO_0000114133"
FT   BINDING         151..158
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   HELIX           1..16
FT                   /evidence="ECO:0007829|PDB:4TPS"
FT   HELIX           21..27
FT                   /evidence="ECO:0007829|PDB:4TPS"
FT   TURN            28..30
FT                   /evidence="ECO:0007829|PDB:4TPS"
FT   STRAND          32..37
FT                   /evidence="ECO:0007829|PDB:4TPS"
FT   STRAND          40..44
FT                   /evidence="ECO:0007829|PDB:4TPS"
FT   HELIX           48..57
FT                   /evidence="ECO:0007829|PDB:4TPS"
FT   HELIX           59..70
FT                   /evidence="ECO:0007829|PDB:4TPS"
FT   STRAND          75..79
FT                   /evidence="ECO:0007829|PDB:4TPS"
SQ   SEQUENCE   446 AA;  50859 MW;  666799A0A183C992 CRC64;
     MENILDLWNQ ALAQIEKKLS KPSFETWMKS TKAHSLQGDT LTITAPNEFA RDWLESRYLH
     LIADTIYELT GEELSIKFVI PQNQDVEDFM PKPQVKKAVK EDTSDFPQNM LNPKYTFDTF
     VIGSGNRFAH AASLAVAEAP AKAYNPLFIY GGVGLGKTHL MHAIGHYVID HNPSAKVVYL
     SSEKFTNEFI NSIRDNKAVD FRNRYRNVDV LLIDDIQFLA GKEQTQEEFF HTFNTLHEES
     KQIVISSDRP PKEIPTLEDR LRSRFEWGLI TDITPPDLET RIAILRKKAK AEGLDIPNEV
     MLYIANQIDS NIRELEGALI RVVAYSSLIN KDINADLAAE ALKDIIPSSK PKVITIKEIQ
     RVVGQQFNIK LEDFKAKKRT KSVAFPRQIA MYLSREMTDS SLPKIGEEFG GRDHTTVIHA
     HEKISKLLAD DEQLQQHVKE IKEQLK