ID   DNAA_BRUME              Reviewed;         496 AA.
AC   Q8YED5;
DT   07-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   07-NOV-2003, sequence version 2.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Chromosomal replication initiator protein DnaA {ECO:0000255|HAMAP-Rule:MF_00377};
GN   Name=dnaA {ECO:0000255|HAMAP-Rule:MF_00377}; OrderedLocusNames=BMEI1943;
OS   Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=224914;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=16M / ATCC 23456 / NCTC 10094;
RX   PubMed=11756688; DOI=10.1073/pnas.221575398;
RA   DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T.,
RA   Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G.,
RA   Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E.,
RA   Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J.,
RA   Haselkorn R., Kyrpides N.C., Overbeek R.;
RT   "The genome sequence of the facultative intracellular pathogen Brucella
RT   melitensis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002).
CC   -!- FUNCTION: Plays an important role in the initiation and regulation of
CC       chromosomal replication. Binds to the origin of replication; it binds
CC       specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-
CC       TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.
CC       {ECO:0000255|HAMAP-Rule:MF_00377}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00377}.
CC   -!- SIMILARITY: Belongs to the DnaA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00377}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL53124.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE008917; AAL53124.1; ALT_INIT; Genomic_DNA.
DR   PIR; AI3494; AI3494.
DR   AlphaFoldDB; Q8YED5; -.
DR   SMR; Q8YED5; -.
DR   STRING; 224914.BMEI1943; -.
DR   PRIDE; Q8YED5; -.
DR   EnsemblBacteria; AAL53124; AAL53124; BMEI1943.
DR   KEGG; bme:BMEI1943; -.
DR   KEGG; bmel:DK63_1548; -.
DR   PATRIC; fig|224914.52.peg.1634; -.
DR   eggNOG; COG0593; Bacteria.
DR   OMA; REFNPLF; -.
DR   Proteomes; UP000000419; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003688; F:DNA replication origin binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006270; P:DNA replication initiation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006275; P:regulation of DNA replication; IEA:UniProtKB-UniRule.
DR   CDD; cd06571; Bac_DnaA_C; 1.
DR   Gene3D; 1.10.1750.10; -; 1.
DR   Gene3D; 3.30.300.180; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00377; DnaA_bact; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR001957; Chromosome_initiator_DnaA.
DR   InterPro; IPR020591; Chromosome_initiator_DnaA-like.
DR   InterPro; IPR018312; Chromosome_initiator_DnaA_CS.
DR   InterPro; IPR013317; DnaA.
DR   InterPro; IPR013159; DnaA_C.
DR   InterPro; IPR024633; DnaA_N_dom.
DR   InterPro; IPR038454; DnaA_N_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR010921; Trp_repressor/repl_initiator.
DR   PANTHER; PTHR30050:SF2; PTHR30050:SF2; 1.
DR   Pfam; PF00308; Bac_DnaA; 1.
DR   Pfam; PF08299; Bac_DnaA_C; 1.
DR   Pfam; PF11638; DnaA_N; 1.
DR   PRINTS; PR00051; DNAA.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00760; Bac_DnaA_C; 1.
DR   SUPFAM; SSF48295; SSF48295; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00362; DnaA; 1.
DR   PROSITE; PS01008; DNAA; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; DNA replication; DNA-binding; Nucleotide-binding.
FT   CHAIN           1..496
FT                   /note="Chromosomal replication initiator protein DnaA"
FT                   /id="PRO_0000114146"
FT   BINDING         194..201
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00377"
SQ   SEQUENCE   496 AA;  55121 MW;  4476E21259505ADA CRC64;
     MKMDSAVSEE AFERLTAKLK ARVGGEIYSS WFGRLKLDDI SKSIVRLSVP TAFLRSWINN
     HYSELLTELW QEENPQILKV EVVVRGVSRV VRSAAPAETC DNAEAKPAVT PREKMVFPVG
     QSFGGQSLGE KRGSAVVAES AAATGAVLGS PLDPRYTFDT FVDGASNRVA LAAARTIAEA
     GSSAVRFNPL FIHASVGLGK THLLQAIAAA ALQRQEKARV VYLTAEYFMW RFATAIRDNN
     ALSFKEQLRD IDLLVIDDMQ FLQGKSIQHE FCHLLNTLLD SAKQVVVAAD RAPSELESLD
     VRVRSRLQGG VALEVAAPDY EMRLEMLRRR LASAQCEDAS LDIGEEILAH VARTVTGSGR
     ELEGAFNQLL FRQSFEPNIS IDRVDELLGH LTRAGEPKRI RIEEIQRIVA RHYNVSKQDL
     LSNRRTRTIV KPRQVAMYLA KMMTPRSLPE IGRRFGGRDH TTVLHAVRKI EDLVGADTKL
     AQELELLKRL INDQAA