ID   ADDA_EXIS2              Reviewed;        1183 AA.
AC   B1YKM8;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=ATP-dependent helicase/nuclease subunit A {ECO:0000255|HAMAP-Rule:MF_01451};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01451};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01451};
DE   AltName: Full=ATP-dependent helicase/nuclease AddA {ECO:0000255|HAMAP-Rule:MF_01451};
GN   Name=addA {ECO:0000255|HAMAP-Rule:MF_01451}; OrderedLocusNames=Exig_0730;
OS   Exiguobacterium sibiricum (strain DSM 17290 / CIP 109462 / JCM 13490 /
OS   255-15).
OC   Bacteria; Firmicutes; Bacilli; Bacillales;
OC   Bacillales Family XII. Incertae Sedis; Exiguobacterium.
OX   NCBI_TaxID=262543;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 17290 / CIP 109462 / JCM 13490 / 255-15;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Kiss H., Chertkov O., Monk C.,
RA   Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F.,
RA   Land M., Hauser L., Kyrpides N., Mikhailova N., Vishnivetskaya T.,
RA   Rodrigues D.F., Gilichinsky D., Tiedje J., Richardson P.;
RT   "Complete sequence of chromosome of Exiguobacterium sibiricum 255-15.";
RL   Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC       and an ATP-dependent, dual-direction single-stranded exonuclease.
CC       Recognizes the chi site generating a DNA molecule suitable for the
CC       initiation of homologous recombination. The AddA nuclease domain is
CC       required for chi fragment generation; this subunit has the helicase and
CC       3' -> 5' nuclease activities. {ECO:0000255|HAMAP-Rule:MF_01451}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC   -!- SUBUNIT: Heterodimer of AddA and AddB/RexB. {ECO:0000255|HAMAP-
CC       Rule:MF_01451}.
CC   -!- SIMILARITY: Belongs to the helicase family. AddA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
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DR   EMBL; CP001022; ACB60211.1; -; Genomic_DNA.
DR   RefSeq; WP_012369635.1; NC_010556.1.
DR   AlphaFoldDB; B1YKM8; -.
DR   SMR; B1YKM8; -.
DR   STRING; 262543.Exig_0730; -.
DR   PRIDE; B1YKM8; -.
DR   EnsemblBacteria; ACB60211; ACB60211; Exig_0730.
DR   KEGG; esi:Exig_0730; -.
DR   eggNOG; COG1074; Bacteria.
DR   HOGENOM; CLU_001114_3_1_9; -.
DR   OMA; KQSIYRW; -.
DR   OrthoDB; 137860at2; -.
DR   Proteomes; UP000001681; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.300; -; 4.
DR   Gene3D; 3.90.320.10; -; 1.
DR   HAMAP; MF_01451; AddA; 1.
DR   InterPro; IPR014152; DNA_helicase_suAddA.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR011604; PDDEXK-like_dom_sf.
DR   InterPro; IPR038726; PDDEXK_AddAB-type.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   PANTHER; PTHR11070:SF48; PTHR11070:SF48; 1.
DR   Pfam; PF12705; PDDEXK_1; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF52980; SSF52980; 1.
DR   TIGRFAMs; TIGR02785; addA_Gpos; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA damage; DNA repair; DNA-binding; Exonuclease; Helicase;
KW   Hydrolase; Nuclease; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..1183
FT                   /note="ATP-dependent helicase/nuclease subunit A"
FT                   /id="PRO_0000379271"
FT   DOMAIN          3..461
FT                   /note="UvrD-like helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT   DOMAIN          473..769
FT                   /note="UvrD-like helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT   BINDING         24..31
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
SQ   SEQUENCE   1183 AA;  136605 MW;  4CF4C1AA49C8F841 CRC64;
     MSVQWTDEQQ RAIDARGGHI LVSAAAGSGK TAVLVERLTQ RVINQEDPLT ADRILVATFT
     NAAAKEMKTR VIEAIEAKIK VAPDDLYLKK QRQMMNRAQI TTIHSFCLSI LRENYYRIGL
     DPAFRIAEEA ELLLLQDDVL EEVFESFYAS ADPAFYELID SYTSDRDDQA MLTLISNLYR
     FSRSLPDPEA FYDHLIAQYD QPIDPDESSL LTRLFELEWE RVGPVINRYM ELSYRLRQSG
     YDEMADLLVQ DVTPVRRINP EQDRWTTVAL AFQAVEFGRW KGIRGDEEMK KFQTERTRLV
     SDLKKMRDLF VEKDGVDYLE DLRSQLGHVE MIVTLVRSFS AAYLEAKQQR GIVDFSDLEH
     FALAILEENG EPTDVARLLQ ERFIEVLVDE YQDTNEVQER ILRLVSKSDE ATGNLFMVGD
     VKQSIYKFRH AEPGLFLNKF KRFQQTEVGT RIDLTKNFRS RLEVLDGTNH IFRQVMDEAV
     GEIDYDEAAY LRLGNLGYVD STQVDPELLL VDQTDTNKEE LEAQVIATRI IEMVNDENPY
     LVFDAKQKRF RKCEYRDIVI LVRSRGKRVQ ALVDVFEQYE LPVYADTTGG YFQATEIQIM
     MSLLKTIDNP LQDIPFASVL RSPIFGLTDR DLGRIRAKSK DGSFYEAAIL VAKEQTPLGL
     RVDEALNQLH HWRTEARGKS LASLIRSLFD QTGYFEYVGC LNGGRSRQAN LNALYERAHQ
     YEASGYRGLY RFLRLIHRLV ERGEDFSEAR SLGEDEDVVR IMTIHQSKGL EFPVTIVSQL
     GKQFNKQDQI QAIQLHKTYG IALDAIDPVK RLRSGTLLKE VIRREMDREM KAEEMRVLYV
     AMTRAKEKLI LVGAIKGLED QLIKWQDQPL DELLLPEMDR RNAKTYADWV VPAVLRNFLL
     SEDGPRWSFR IIALDEIAPY QELTKMVEQL EHVRVLEKID HAGDETLNIQ IEAAFAYQYP
     YQVATDTAAK QTVTELKRTE QLERAAFEST HRQSTYYRTP QFLGPTLTGA ERGTVLHLAM
     QLYESGRSFE EQILDWEQAE RISSLEAQTM REAIPELTTF LASDTGQLFE QRLLAGEVYR
     ELPFTYKIDS ARFRSDWHGP SDQAVMQGIV DCLIRDGDTY ILLDYKSDQV FETTDNQNQA
     EVLRTRYATQ LNLYQEALEA ILHIRISRKL IYAFALHEVI EIY