ADDA_GEOKA
ID ADDA_GEOKA Reviewed; 1242 AA.
AC Q5L263;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=ATP-dependent helicase/nuclease subunit A {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01451};
DE AltName: Full=ATP-dependent helicase/nuclease AddA {ECO:0000255|HAMAP-Rule:MF_01451};
GN Name=addA {ECO:0000255|HAMAP-Rule:MF_01451}; OrderedLocusNames=GK0682;
OS Geobacillus kaustophilus (strain HTA426).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus;
OC Geobacillus thermoleovorans group.
OX NCBI_TaxID=235909;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HTA426;
RX PubMed=15576355; DOI=10.1093/nar/gkh970;
RA Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H.,
RA Matsui S., Uchiyama I.;
RT "Thermoadaptation trait revealed by the genome sequence of thermophilic
RT Geobacillus kaustophilus.";
RL Nucleic Acids Res. 32:6292-6303(2004).
CC -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC and an ATP-dependent, dual-direction single-stranded exonuclease.
CC Recognizes the chi site generating a DNA molecule suitable for the
CC initiation of homologous recombination. The AddA nuclease domain is
CC required for chi fragment generation; this subunit has the helicase and
CC 3' -> 5' nuclease activities. {ECO:0000255|HAMAP-Rule:MF_01451}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- SUBUNIT: Heterodimer of AddA and AddB/RexB. {ECO:0000255|HAMAP-
CC Rule:MF_01451}.
CC -!- SIMILARITY: Belongs to the helicase family. AddA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01451}.
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DR EMBL; BA000043; BAD74967.1; -; Genomic_DNA.
DR RefSeq; WP_011230185.1; NC_006510.1.
DR AlphaFoldDB; Q5L263; -.
DR SMR; Q5L263; -.
DR STRING; 235909.GK0682; -.
DR EnsemblBacteria; BAD74967; BAD74967; GK0682.
DR KEGG; gka:GK0682; -.
DR PATRIC; fig|235909.7.peg.761; -.
DR eggNOG; COG1074; Bacteria.
DR HOGENOM; CLU_001114_3_1_9; -.
DR OMA; KQSIYRW; -.
DR Proteomes; UP000001172; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 3.
DR Gene3D; 3.90.320.10; -; 1.
DR HAMAP; MF_01451; AddA; 1.
DR InterPro; IPR014152; DNA_helicase_suAddA.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR PANTHER; PTHR11070:SF48; PTHR11070:SF48; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
DR TIGRFAMs; TIGR02785; addA_Gpos; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Exonuclease; Helicase;
KW Hydrolase; Nuclease; Nucleotide-binding; Reference proteome.
FT CHAIN 1..1242
FT /note="ATP-dependent helicase/nuclease subunit A"
FT /id="PRO_0000379272"
FT DOMAIN 12..487
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT DOMAIN 514..808
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT BINDING 33..40
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
SQ SEQUENCE 1242 AA; 141270 MW; E72E9F88B98284F2 CRC64;
MNATIRPKPA GSRWTDEQWK AIAAGGRDIL VAAAAGSGKT AVLVERIIQK VTAEEGAVDI
DRLLVVTFTN AAAAEMKARI GEALERELAK RPHSLHLRRQ LSLLPRAAIS TLHSFCLDVI
RKYYYLLDLD PSFRIADETE IELLKEDVLE ELLEEEYGKP DNERFFAVVD AYTGDRSDAE
LQEMIVALYE FSRSHPEPDE WLAGLTSMYD VDERTDIKTL PAARYIAQHA AMELAAARRL
IRRALELAEE PGGPRPYAER LREDRDMITD LETRLSGPWA ELHRALKALS FGRLPACRGK
DYDERLIDEA KSLRDQAKKK VEALRDNVFS LDPSVWLRHM REMKPIVETI ANLVRRFAVM
FQAAKREKGI VDFSDLEHYC LHILRRRDPE TGEWQPSPAA LEYQAQFDEV LVDEYQDTNL
VQEAILQLVK KGSERTGNLF MVGDVKQSIY RFRLAEPMLF LDKYKRFTAD GEEGGMKIDL
ASNFRSRREV LDGTNFLFAQ LMGETVGEMV YDEAAQLKYG ADYPEGEDAA PEVMIINRQR
AAEEDEEEAA EWEAAELEAR LMAKKIKEIV SAPFYVYDRS SGQPRRAMYR DIVILVRSMT
NAPQMIEQLQ AQGIPAAADL SSGYFQATEI SIMLSLLKVI DNPHQDIPLA AVLRSPLFRF
DENELAMIRL ADPKGTFYEA LCSFRQKPAE TKEEANAQRK AAAFLERLEG WRTMARRRSL
ADLIWQLYRD TQFYDFVGAL PGGRQRQANL RALYDRARQY ESTSFRGLFR FLRFIERLQE
RGDDLGAARP LGEQEDVVRI MTIHSSKGLE FPIVFLAGLA RPFHTRDLHH PYLLDKELGF
AARFVHPRLR ISYPTLPLLA IQTKKRLELL AEEMRILYVA LTRAKEKLYL LASVNDAAKE
IEKWKSAASE RGWLLPDDVR ASARSYLDWI GRALIRHRDG GALAGTKAPE EVASHPSVWR
VAIVPAADLR GAEAAREEMD GGVLLALEQG RPVPVEGGWQ KEVKRRLLWR YSYEKETAVR
AKQSVSELKE QRALFGEQAD EWRPRQGTAP VFSRPRFMQE KTLTPAEKGT ALHVVMRHLD
LQAPLDESWI RSQIVRLVEK ELLSAEQAEA VDPAAIAAFF TADLGRRLCA AREVHREVPF
SLGLKAAELY GGEGTESGRR VLVQGVIDCV FADECGYVMI DYKTDEVVHR FAGQKEEAAR
FLLGRYGTQM RLYRRAIEQI WRAPVAECYL YSFDGGFVVA VE
