ADDA_GEOTN
ID ADDA_GEOTN Reviewed; 1242 AA.
AC A4IKW7;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2007, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=ATP-dependent helicase/nuclease subunit A {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01451};
DE AltName: Full=ATP-dependent helicase/nuclease AddA {ECO:0000255|HAMAP-Rule:MF_01451};
GN Name=addA {ECO:0000255|HAMAP-Rule:MF_01451}; OrderedLocusNames=GTNG_0589;
OS Geobacillus thermodenitrificans (strain NG80-2).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX NCBI_TaxID=420246;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NG80-2;
RX PubMed=17372208; DOI=10.1073/pnas.0609650104;
RA Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X.,
RA Han W., Peng X., Liu R., Wang L.;
RT "Genome and proteome of long-chain alkane degrading Geobacillus
RT thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007).
CC -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC and an ATP-dependent, dual-direction single-stranded exonuclease.
CC Recognizes the chi site generating a DNA molecule suitable for the
CC initiation of homologous recombination. The AddA nuclease domain is
CC required for chi fragment generation; this subunit has the helicase and
CC 3' -> 5' nuclease activities. {ECO:0000255|HAMAP-Rule:MF_01451}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- SUBUNIT: Heterodimer of AddA and AddB/RexB. {ECO:0000255|HAMAP-
CC Rule:MF_01451}.
CC -!- SIMILARITY: Belongs to the helicase family. AddA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01451}.
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DR EMBL; CP000557; ABO65971.1; -; Genomic_DNA.
DR RefSeq; WP_011886886.1; NC_009328.1.
DR AlphaFoldDB; A4IKW7; -.
DR SMR; A4IKW7; -.
DR STRING; 420246.GTNG_0589; -.
DR PRIDE; A4IKW7; -.
DR EnsemblBacteria; ABO65971; ABO65971; GTNG_0589.
DR KEGG; gtn:GTNG_0589; -.
DR eggNOG; COG1074; Bacteria.
DR HOGENOM; CLU_001114_3_1_9; -.
DR OMA; KQSIYRW; -.
DR OrthoDB; 137860at2; -.
DR Proteomes; UP000001578; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 4.
DR Gene3D; 3.90.320.10; -; 1.
DR HAMAP; MF_01451; AddA; 1.
DR InterPro; IPR014152; DNA_helicase_suAddA.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR PANTHER; PTHR11070:SF48; PTHR11070:SF48; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
DR TIGRFAMs; TIGR02785; addA_Gpos; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Exonuclease; Helicase;
KW Hydrolase; Nuclease; Nucleotide-binding.
FT CHAIN 1..1242
FT /note="ATP-dependent helicase/nuclease subunit A"
FT /id="PRO_0000379273"
FT DOMAIN 12..487
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT DOMAIN 514..808
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT BINDING 33..40
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
SQ SEQUENCE 1242 AA; 141543 MW; EB322B798EE48052 CRC64;
MNATFRPKPA GSRWTDEQWK AIAAGGRDIL VAAAAGSGKT AVLVERIIQK VTAEEGAVDI
DRLLVVTFTN AAAAEMKARI GEALERELAN RPHSLHLRRQ LSLLNRASIS TLHSFCLDVI
RKYYYLLDLD PSFRIADETE IELLKEDVLE ELLEEEYGKA DNERFFAVVD AYTGDRSDAE
LQEMILALYE FSRSHPAPDE WLADLVSMYD VDEQTNVETL PPARYIAQHA AMELAAAKRF
IGLALALAEK ESGPKPYEKR LREDMDLIAD LERRLSGSWD ELYHALQSLS FGRLPPCRGD
GFDAELIDEA KSLRDQAKKK IEALRDNVFS LHPSAWLRHM REMKPVVETI AALVRRFSAM
FEAAKREKGI VDFSDLEHYC LRILRQYDPE TGEWQPSSAA LEYQAQFDEV LVDEYQDTNL
VQETILQLVK KGSERTGNLF MVGDVKQSIY RFRLAEPMLF LDKYKRFTAD GEAGGMKIDL
ASNFRSRAEV LDGTNFLFAQ IMGEAVGEMV YDEAAQLKYG ADYPEGTDAV PEVMIIDRQR
TSEEDEEETA ELEAAELESR LMAEKIKEIV SRPFYVYDRS SGQQRRAMYR DIVVLVRSMT
NAPQMIEQLQ AQGIPAAADL SSGYFQATEI SVMLSLLKVI DNPYQDIPLA AVLRSPLFRF
DENELAMIRL SDPKGTFFEA LQAFCQKTAE TGEEENAKEK AISFLNKLEE WRTMARRRSL
ADLIWQLYRD TQFYDFVGAL PGGKQRQANL RALYDRARQY ESTSFRGLFR FLRFIERLQE
RGDDLGAARP LGDQEDVVRV MTIHSSKGLE FPIVFLVGLA RPFYTRDLHS PYLLDKELGF
AARFVHPRLR ISYPTLPLLA IQVKKRLELL AEEMRILYVA LTRAKEKLYL LASVNDADKE
IEKWKGVAAE SGWLLPDDVR ASARSYLDWI GRALIRHRDG RSLVEVNRPD EIASHPSVWR
FEIVSAAKLR NAEVSTDRED GGALVALEQG RPVPTQGEWE EEARRRLLWR YRYGKETVVR
AKQSVSELKE QQALFGEQAD EWLPRKGTAP LFSRPRFMQE KTLTPAEKGT ALHVVMRHLD
LHAPMDESSI RSQIIRLVEK ELLSAEQAET VDAAAIVAFF ATDIGRRLCA AREVYREVPF
SLGLPADELY GSEGMESGRR LLVQGVVDCV FADERGYVVI DYKTDEVTGR FAGQKEEATR
FLLGRYGGQM RLYRRAIEQI WRVPVAECYL YSFDGEYFLA VE
