DNAA_MYCGE
ID DNAA_MYCGE Reviewed; 437 AA.
AC P35888; Q49363;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Chromosomal replication initiator protein DnaA;
GN Name=dnaA; OrderedLocusNames=MG469;
OS Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)
OS (Mycoplasmoides genitalium).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX NCBI_TaxID=243273;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=7569993; DOI=10.1126/science.270.5235.397;
RA Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT "The minimal gene complement of Mycoplasma genitalium.";
RL Science 270:397-403(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 183-281 AND 286-402.
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=8253680; DOI=10.1128/jb.175.24.7918-7930.1993;
RA Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III;
RT "A survey of the Mycoplasma genitalium genome by using random sequencing.";
RL J. Bacteriol. 175:7918-7930(1993).
CC -!- FUNCTION: Plays an important role in the initiation and regulation of
CC chromosomal replication. Binds to the origin of replication; it binds
CC specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-
CC TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DnaA family. {ECO:0000305}.
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DR EMBL; L43967; AAC72490.1; -; Genomic_DNA.
DR EMBL; U02259; AAD12524.1; -; Genomic_DNA.
DR EMBL; U02145; AAD12425.1; -; Genomic_DNA.
DR PIR; H64251; H64251.
DR RefSeq; WP_010869493.1; NC_000908.2.
DR PDB; 2JMP; NMR; -; A=1-100.
DR PDBsum; 2JMP; -.
DR AlphaFoldDB; P35888; -.
DR BMRB; P35888; -.
DR SMR; P35888; -.
DR STRING; 243273.MG_469; -.
DR EnsemblBacteria; AAC72490; AAC72490; MG_469.
DR KEGG; mge:MG_469; -.
DR eggNOG; COG0593; Bacteria.
DR HOGENOM; CLU_026910_3_2_14; -.
DR OMA; REFNPLF; -.
DR OrthoDB; 219876at2; -.
DR BioCyc; MGEN243273:G1GJ2-564-MON; -.
DR EvolutionaryTrace; P35888; -.
DR Proteomes; UP000000807; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR GO; GO:0006275; P:regulation of DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd06571; Bac_DnaA_C; 1.
DR Gene3D; 1.10.1750.10; -; 1.
DR Gene3D; 3.30.300.20; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00377; DnaA_bact; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR001957; Chromosome_initiator_DnaA.
DR InterPro; IPR020591; Chromosome_initiator_DnaA-like.
DR InterPro; IPR018312; Chromosome_initiator_DnaA_CS.
DR InterPro; IPR013317; DnaA.
DR InterPro; IPR013159; DnaA_C.
DR InterPro; IPR024633; DnaA_N_dom.
DR InterPro; IPR015946; KH_dom-like_a/b.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR010921; Trp_repressor/repl_initiator.
DR PANTHER; PTHR30050:SF2; PTHR30050:SF2; 1.
DR Pfam; PF00308; Bac_DnaA; 1.
DR Pfam; PF08299; Bac_DnaA_C; 1.
DR Pfam; PF11638; DnaA_N; 1.
DR PRINTS; PR00051; DNAA.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00760; Bac_DnaA_C; 1.
DR SUPFAM; SSF48295; SSF48295; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00362; DnaA; 1.
DR PROSITE; PS01008; DNAA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cytoplasm; DNA replication; DNA-binding;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..437
FT /note="Chromosomal replication initiator protein DnaA"
FT /id="PRO_0000114211"
FT BINDING 141..148
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 198
FT /note="E -> G (in Ref. 2; AAD12524)"
FT /evidence="ECO:0000305"
FT HELIX 2..14
FT /evidence="ECO:0007829|PDB:2JMP"
FT HELIX 19..24
FT /evidence="ECO:0007829|PDB:2JMP"
FT TURN 25..28
FT /evidence="ECO:0007829|PDB:2JMP"
FT STRAND 32..34
FT /evidence="ECO:0007829|PDB:2JMP"
FT STRAND 36..42
FT /evidence="ECO:0007829|PDB:2JMP"
FT HELIX 46..54
FT /evidence="ECO:0007829|PDB:2JMP"
FT HELIX 59..62
FT /evidence="ECO:0007829|PDB:2JMP"
FT TURN 63..65
FT /evidence="ECO:0007829|PDB:2JMP"
FT STRAND 71..75
FT /evidence="ECO:0007829|PDB:2JMP"
FT HELIX 77..85
FT /evidence="ECO:0007829|PDB:2JMP"
SQ SEQUENCE 437 AA; 50762 MW; D4DF45A0F4768ED6 CRC64;
MEQFNAFKSL LKKHYEKTIG FHDKYIKDIN RFVFKNNVLL ILLENEFARN SLNDNSEIIH
LAESLYEGIK SVNFVNEQDF FFNLAKLEEN SRDTLYQNSG LSKNYTFQNF VISEGNKRAY
EAGVRLAETQ DNEFSPLFIY GETGLGKTHL LQAIGNEKFR NFPNARVKYV VSSDFAQEVV
DAFYQRDKGI EKLKKNYENL DLVLIDDTQI FGRKEKTLEI LFNIFNNLVL NKKQIVLVSD
KAPDELIDID ARMISRFKSG LLLKIEKHNL SSLCEILTVK LKEKDPNIQI TNEARHDAAQ
ISGNDVRALN GIATKLLFFA KTSKQNLINT ENLKEILFEE FEKFHKKSFD PYLLIENVCR
RFNVPMDSVL SENRKAELVR VRDVCNYLLR QKYNMQFQQI GKIFKRSHSS VLMAVKRVAK
MIENDSSLRD VITSLVI
