DNAA_MYCS2
ID DNAA_MYCS2 Reviewed; 504 AA.
AC A0R7K1; I7G121; P49992; Q59558;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Chromosomal replication initiator protein DnaA;
GN Name=dnaA; OrderedLocusNames=MSMEG_6947, MSMEI_0001;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7592430; DOI=10.1128/jb.177.22.6527-6535.1995;
RA Rajagopalan M., Qin M.H., Nash D.R., Madiraju M.V.V.S.;
RT "Mycobacterium smegmatis dnaA region and autonomous replication activity.";
RL J. Bacteriol. 177:6527-6535(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [5]
RP INDUCTION.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=22610443; DOI=10.1074/jbc.m112.346544;
RA Plocinska R., Purushotham G., Sarva K., Vadrevu I.S., Pandeeti E.V.,
RA Arora N., Plocinski P., Madiraju M.V., Rajagopalan M.;
RT "Septal localization of the Mycobacterium tuberculosis MtrB sensor kinase
RT promotes MtrA regulon expression.";
RL J. Biol. Chem. 287:23887-23899(2012).
CC -!- FUNCTION: Plays an important role in the initiation and regulation of
CC chromosomal replication. Binds to the origin of replication; it binds
CC specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-
CC TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- INDUCTION: Expression depends on the two-component regulatory system
CC MtrA/MtrB. {ECO:0000269|PubMed:22610443}.
CC -!- SIMILARITY: Belongs to the DnaA family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC43711.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; U17833; AAC43711.1; ALT_FRAME; Genomic_DNA.
DR EMBL; CP000480; ABK76223.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP36486.1; -; Genomic_DNA.
DR RefSeq; WP_003898364.1; NZ_SIJM01000001.1.
DR RefSeq; YP_891139.1; NC_008596.1.
DR AlphaFoldDB; A0R7K1; -.
DR SMR; A0R7K1; -.
DR STRING; 246196.MSMEI_0001; -.
DR PRIDE; A0R7K1; -.
DR EnsemblBacteria; ABK76223; ABK76223; MSMEG_6947.
DR EnsemblBacteria; AFP36486; AFP36486; MSMEI_0001.
DR GeneID; 66738192; -.
DR KEGG; msg:MSMEI_0001; -.
DR KEGG; msm:MSMEG_6947; -.
DR PATRIC; fig|246196.19.peg.6759; -.
DR eggNOG; COG0593; Bacteria.
DR OMA; REFNPLF; -.
DR OrthoDB; 219876at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003688; F:DNA replication origin binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006270; P:DNA replication initiation; IEA:UniProtKB-UniRule.
DR GO; GO:0006275; P:regulation of DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd06571; Bac_DnaA_C; 1.
DR Gene3D; 1.10.1750.10; -; 1.
DR Gene3D; 3.30.300.180; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00377; DnaA_bact; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR001957; Chromosome_initiator_DnaA.
DR InterPro; IPR020591; Chromosome_initiator_DnaA-like.
DR InterPro; IPR018312; Chromosome_initiator_DnaA_CS.
DR InterPro; IPR013317; DnaA.
DR InterPro; IPR013159; DnaA_C.
DR InterPro; IPR038454; DnaA_N_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR010921; Trp_repressor/repl_initiator.
DR PANTHER; PTHR30050:SF2; PTHR30050:SF2; 1.
DR Pfam; PF00308; Bac_DnaA; 1.
DR Pfam; PF08299; Bac_DnaA_C; 1.
DR PRINTS; PR00051; DNAA.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00760; Bac_DnaA_C; 1.
DR SUPFAM; SSF48295; SSF48295; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00362; DnaA; 1.
DR PROSITE; PS01008; DNAA; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cytoplasm; DNA replication; DNA-binding; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..504
FT /note="Chromosomal replication initiator protein DnaA"
FT /id="PRO_0000293597"
FT REGION 98..162
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 205..212
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 218
FT /note="A -> H (in Ref. 1; AAC43711)"
FT /evidence="ECO:0000305"
FT CONFLICT 234
FT /note="V -> A (in Ref. 1; AAC43711)"
FT /evidence="ECO:0000305"
FT CONFLICT 315
FT /note="L -> F (in Ref. 1; AAC43711)"
FT /evidence="ECO:0000305"
FT CONFLICT 447
FT /note="L -> M (in Ref. 1; AAC43711)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 504 AA; 56664 MW; C7609F2A6D4030CB CRC64;
MTADPDPPFV AVWNSVVAEL NGDVNGDRQG DPSLPVLTPQ QRAWLKLVKP LVIAEGFALL
SVPTPFVQNE IERHLREPIV TALSRKLGQR VELGVRIATP TDEPEDAPDS FADSPAPASV
PAGPADADEI DDDRDARVNA QESWPKYFSR PEPDTSSDDS NAVNLNRRYT FDTFVIGASN
RFAHAATLAI AEAPARAYNP LFIWGESGLG KTHLLHAAGN YAQRLFPGMR VKYVSTEEFT
NDFINSLRDD RKASFKRSYR DIDILLVDDI QFIEGKEGIQ EEFFHTFNTL HNSNKQIVIS
SDRPPKQLAT LEDRLRTRFE WGLITDVQPP ELETRIAILR KKAQMDRLDV PDDVLELIAS
SIERNIRELE GALIRVTAFA SLNKTRIDRS LAEVVLRDLI ADATTMQIST AAIMAVTAEY
FETTVEELRG PGKTRALAQS RQIAMYLCRE LTDLSLPKIG QAFGRDHTTV MYAEKKIRGE
MAERREVFDH VKELTTRIRQ RAKR
