ID   DNAA_MYCTA              Reviewed;         507 AA.
AC   A5TY69;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Chromosomal replication initiator protein DnaA {ECO:0000255|HAMAP-Rule:MF_00377};
GN   Name=dnaA {ECO:0000255|HAMAP-Rule:MF_00377}; OrderedLocusNames=MRA_0001;
OS   Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=419947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25177 / H37Ra;
RX   PubMed=18584054; DOI=10.1371/journal.pone.0002375;
RA   Zheng H., Lu L., Wang B., Pu S., Zhang X., Zhu G., Shi W., Zhang L.,
RA   Wang H., Wang S., Zhao G., Zhang Y.;
RT   "Genetic basis of virulence attenuation revealed by comparative genomic
RT   analysis of Mycobacterium tuberculosis strain H37Ra versus H37Rv.";
RL   PLoS ONE 3:E2375-E2375(2008).
CC   -!- FUNCTION: Plays an important role in the initiation and regulation of
CC       chromosomal replication. Binds to the origin of replication; it binds
CC       specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-
CC       TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.
CC       {ECO:0000255|HAMAP-Rule:MF_00377}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00377}.
CC   -!- SIMILARITY: Belongs to the DnaA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00377}.
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DR   EMBL; CP000611; ABQ71719.1; -; Genomic_DNA.
DR   RefSeq; WP_003400253.1; NZ_CP016972.1.
DR   PDB; 3PVP; X-ray; 2.30 A; A/B=411-507.
DR   PDB; 3PVV; X-ray; 2.00 A; A/B=411-507.
DR   PDBsum; 3PVP; -.
DR   PDBsum; 3PVV; -.
DR   AlphaFoldDB; A5TY69; -.
DR   SMR; A5TY69; -.
DR   STRING; 419947.MRA_0001; -.
DR   PRIDE; A5TY69; -.
DR   EnsemblBacteria; ABQ71719; ABQ71719; MRA_0001.
DR   GeneID; 45423958; -.
DR   KEGG; mra:MRA_0001; -.
DR   eggNOG; COG0593; Bacteria.
DR   HOGENOM; CLU_026910_2_0_11; -.
DR   OMA; REFNPLF; -.
DR   OrthoDB; 219876at2; -.
DR   EvolutionaryTrace; A5TY69; -.
DR   Proteomes; UP000001988; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003688; F:DNA replication origin binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006270; P:DNA replication initiation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006275; P:regulation of DNA replication; IEA:UniProtKB-UniRule.
DR   CDD; cd06571; Bac_DnaA_C; 1.
DR   Gene3D; 1.10.1750.10; -; 1.
DR   Gene3D; 3.30.300.180; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00377; DnaA_bact; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR001957; Chromosome_initiator_DnaA.
DR   InterPro; IPR020591; Chromosome_initiator_DnaA-like.
DR   InterPro; IPR018312; Chromosome_initiator_DnaA_CS.
DR   InterPro; IPR013317; DnaA.
DR   InterPro; IPR013159; DnaA_C.
DR   InterPro; IPR038454; DnaA_N_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR010921; Trp_repressor/repl_initiator.
DR   PANTHER; PTHR30050:SF2; PTHR30050:SF2; 1.
DR   Pfam; PF00308; Bac_DnaA; 1.
DR   Pfam; PF08299; Bac_DnaA_C; 1.
DR   PRINTS; PR00051; DNAA.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00760; Bac_DnaA_C; 1.
DR   SUPFAM; SSF48295; SSF48295; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00362; DnaA; 1.
DR   PROSITE; PS01008; DNAA; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; Cytoplasm; DNA replication; DNA-binding;
KW   Nucleotide-binding.
FT   CHAIN           1..507
FT                   /note="Chromosomal replication initiator protein DnaA"
FT                   /id="PRO_1000048674"
FT   REGION          99..155
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        111..127
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         208..215
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00377"
FT   HELIX           413..423
FT                   /evidence="ECO:0007829|PDB:3PVV"
FT   HELIX           428..432
FT                   /evidence="ECO:0007829|PDB:3PVV"
FT   HELIX           438..454
FT                   /evidence="ECO:0007829|PDB:3PVV"
FT   HELIX           459..465
FT                   /evidence="ECO:0007829|PDB:3PVV"
FT   HELIX           470..486
FT                   /evidence="ECO:0007829|PDB:3PVV"
FT   HELIX           488..505
FT                   /evidence="ECO:0007829|PDB:3PVV"
SQ   SEQUENCE   507 AA;  56567 MW;  C4F252150D453A00 CRC64;
     MTDDPGSGFT TVWNAVVSEL NGDPKVDDGP SSDANLSAPL TPQQRAWLNL VQPLTIVEGF
     ALLSVPSSFV QNEIERHLRA PITDALSRRL GHQIQLGVRI APPATDEADD TTVPPSENPA
     TTSPDTTTDN DEIDDSAAAR GDNQHSWPSY FTERPHNTDS ATAGVTSLNR RYTFDTFVIG
     ASNRFAHAAA LAIAEAPARA YNPLFIWGES GLGKTHLLHA AGNYAQRLFP GMRVKYVSTE
     EFTNDFINSL RDDRKVAFKR SYRDVDVLLV DDIQFIEGKE GIQEEFFHTF NTLHNANKQI
     VISSDRPPKQ LATLEDRLRT RFEWGLITDV QPPELETRIA ILRKKAQMER LAVPDDVLEL
     IASSIERNIR ELEGALIRVT AFASLNKTPI DKALAEIVLR DLIADANTMQ ISAATIMAAT
     AEYFDTTVEE LRGPGKTRAL AQSRQIAMYL CRELTDLSLP KIGQAFGRDH TTVMYAQRKI
     LSEMAERREV FDHVKELTTR IRQRSKR