ADDA_LACJO
ID ADDA_LACJO Reviewed; 1204 AA.
AC Q74JA6;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=ATP-dependent helicase/nuclease subunit A {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01451};
DE AltName: Full=ATP-dependent helicase/nuclease AddA {ECO:0000255|HAMAP-Rule:MF_01451};
GN Name=addA {ECO:0000255|HAMAP-Rule:MF_01451}; OrderedLocusNames=LJ_1203;
OS Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactobacillus.
OX NCBI_TaxID=257314;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CNCM I-1225 / La1 / NCC 533;
RX PubMed=14983040; DOI=10.1073/pnas.0307327101;
RA Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C.,
RA Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R.,
RA Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.;
RT "The genome sequence of the probiotic intestinal bacterium Lactobacillus
RT johnsonii NCC 533.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004).
CC -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC and an ATP-dependent, dual-direction single-stranded exonuclease.
CC Recognizes the chi site generating a DNA molecule suitable for the
CC initiation of homologous recombination. The AddA nuclease domain is
CC required for chi fragment generation; this subunit has the helicase and
CC 3' -> 5' nuclease activities. {ECO:0000255|HAMAP-Rule:MF_01451}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- SUBUNIT: Heterodimer of AddA and AddB/RexB. {ECO:0000255|HAMAP-
CC Rule:MF_01451}.
CC -!- SIMILARITY: Belongs to the helicase family. AddA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01451}.
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DR EMBL; AE017198; AAS09024.1; -; Genomic_DNA.
DR RefSeq; WP_011162032.1; NC_005362.1.
DR AlphaFoldDB; Q74JA6; -.
DR SMR; Q74JA6; -.
DR STRING; 257314.LJ_1203; -.
DR PRIDE; Q74JA6; -.
DR EnsemblBacteria; AAS09024; AAS09024; LJ_1203.
DR KEGG; ljo:LJ_1203; -.
DR PATRIC; fig|257314.6.peg.1069; -.
DR eggNOG; COG1074; Bacteria.
DR HOGENOM; CLU_001114_3_1_9; -.
DR OMA; KQSIYRW; -.
DR Proteomes; UP000000581; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 4.
DR Gene3D; 3.90.320.10; -; 1.
DR HAMAP; MF_01451; AddA; 1.
DR InterPro; IPR014152; DNA_helicase_suAddA.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR PANTHER; PTHR11070:SF48; PTHR11070:SF48; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
DR TIGRFAMs; TIGR02785; addA_Gpos; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Exonuclease; Helicase;
KW Hydrolase; Nuclease; Nucleotide-binding; Reference proteome.
FT CHAIN 1..1204
FT /note="ATP-dependent helicase/nuclease subunit A"
FT /id="PRO_0000379284"
FT DOMAIN 2..469
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT DOMAIN 496..784
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT BINDING 23..30
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
SQ SEQUENCE 1204 AA; 139871 MW; 6049198AB96EA162 CRC64;
MTKFTKEQNQ AINDYGKDIL VSASAGSGKT TVLVERVLKR ILSGTPVSSL LIITFTKAAA
REMKERIKQK ISDQIEKEPN NQFLRSQLLD VDTANISTID SFCLDVIRRF YYVIDLDPQF
SVLTDETQAE LLKERALHEI EIEYLEKNDQ DFQDFYDNFS GDRDAEGARN LLLQLYNTVV
TEPNYEKFLN NLPNFYQVQD DLIESDLWQT QIKPLLIKEI KDLQTEIRQF FENPQMENPD
LVKVKENYDI FTSRLEQFLN ALEDDHSYNE IRASLMNCKF EKNIRKSKKW SEESLETYQE
SQKLKSDLND QLKKIFANFF VVEEKEQVNI LKKSEKLVKT IVDAEKKLIK RFGQLKREQN
LIDYSDMEQF AFSILTTDTS NAHIAQEYYQ EKFNEILIDE YQDVNALQEN IIAAIKKKGQ
NNLFMVGDIK QSIYGFRQAR PDLFLSKYHA YGQNDDSEKI VLSDNFRSTQ RVTKTVNSLF
NPILTANFGG IDYKKEGQLQ FGATYYPTDL PTASEYIFTD KKQTQASFEE NFGDEMDFSE
IQMVIARIKQ LKEENFQVWD RKTQLKRPLE YSDIAIITRT RSDNLQVMQE FAKADLPLFV
TDAQNYFQTF ELVMIMNYLR LIDNPQQDIP LVAVLRSPLF NFKEPELAQI RVKTRSGNFY
NALTSFASVN SDLGQKCKNF LQQLESLRSF AATHRISELI WSIYERTHLL EIVTGLPNGQ
QRRVNLESLY ERATSYESAG FKGLYQFISF IERMRKNQKD LAQPLLSDKA DKAVKLMTIH
ASKGLEFPVV FVMGLGHKYQ TRDLSGNFTI SKDGLGLTIK EKDYRIDSLV KSLADVEKRQ
QMLEEEARIL YVGLTRAQQK LILVASVSEM EAKQKKWESE IDQKTNILPL IRKINAQSPL
DFLGPKLEQK HEFDQTIEDM TLALEEQDKI YYLKFAVQSD IEEKDEQKDD TQKLSSKMND
VVKTLYNFEY PFADATKTTA YQSVSEIKKV FNDPMDTELE NSRLISSSNR YLQPIDETPV
FLEKQKFTGA EIGTAMHLVL QYYDYQGDKT EINLEQEIEE LVELGKLNPL MVPHLSKEAL
NWFVMSEFAA EFWQKPEKLH RESQFSSLVN ASELFNDFSD SAAKILVHGT IDGYFETDEG
LILFDYKTDF VDKTHEEQAI DKIKKKYTGQ LRLYEQALNE ISENKKVIGK YLILLDARKV
VPVD
