ADDA_LACPL
ID ADDA_LACPL Reviewed; 1249 AA.
AC Q88U41; F9URH6;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=ATP-dependent helicase/nuclease subunit A {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01451};
DE AltName: Full=ATP-dependent helicase/nuclease AddA {ECO:0000255|HAMAP-Rule:MF_01451};
GN Name=addA {ECO:0000255|HAMAP-Rule:MF_01451}; Synonyms=rexA;
GN OrderedLocusNames=lp_2693;
OS Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
OS (Lactobacillus plantarum).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactiplantibacillus.
OX NCBI_TaxID=220668;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX PubMed=12566566; DOI=10.1073/pnas.0337704100;
RA Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P.,
RA Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J.,
RA Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M.,
RA Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M.,
RA Siezen R.J.;
RT "Complete genome sequence of Lactobacillus plantarum WCFS1.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX PubMed=22156394; DOI=10.1128/jb.06275-11;
RA Siezen R.J., Francke C., Renckens B., Boekhorst J., Wels M.,
RA Kleerebezem M., van Hijum S.A.;
RT "Complete resequencing and reannotation of the Lactobacillus plantarum
RT WCFS1 genome.";
RL J. Bacteriol. 194:195-196(2012).
CC -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC and an ATP-dependent, dual-direction single-stranded exonuclease.
CC Recognizes the chi site generating a DNA molecule suitable for the
CC initiation of homologous recombination. The AddA nuclease domain is
CC required for chi fragment generation; this subunit has the helicase and
CC 3' -> 5' nuclease activities. {ECO:0000255|HAMAP-Rule:MF_01451}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- SUBUNIT: Heterodimer of AddA and AddB/RexB. {ECO:0000255|HAMAP-
CC Rule:MF_01451}.
CC -!- SIMILARITY: Belongs to the helicase family. AddA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01451}.
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DR EMBL; AL935263; CCC79815.1; -; Genomic_DNA.
DR RefSeq; WP_011101882.1; NC_004567.2.
DR RefSeq; YP_004890329.1; NC_004567.2.
DR AlphaFoldDB; Q88U41; -.
DR SMR; Q88U41; -.
DR STRING; 220668.lp_2693; -.
DR PRIDE; Q88U41; -.
DR EnsemblBacteria; CCC79815; CCC79815; lp_2693.
DR KEGG; lpl:lp_2693; -.
DR PATRIC; fig|220668.9.peg.2254; -.
DR eggNOG; COG1074; Bacteria.
DR HOGENOM; CLU_001114_3_1_9; -.
DR OMA; KQSIYRW; -.
DR PhylomeDB; Q88U41; -.
DR BioCyc; LPLA220668:G1GW0-2306-MON; -.
DR Proteomes; UP000000432; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 4.
DR Gene3D; 3.90.320.10; -; 1.
DR HAMAP; MF_01451; AddA; 1.
DR InterPro; IPR014152; DNA_helicase_suAddA.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR PANTHER; PTHR11070:SF48; PTHR11070:SF48; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
DR TIGRFAMs; TIGR02785; addA_Gpos; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Exonuclease; Helicase;
KW Hydrolase; Nuclease; Nucleotide-binding; Reference proteome.
FT CHAIN 1..1249
FT /note="ATP-dependent helicase/nuclease subunit A"
FT /id="PRO_0000379285"
FT DOMAIN 5..482
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT DOMAIN 521..811
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT BINDING 26..33
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
SQ SEQUENCE 1249 AA; 141029 MW; 0C270367B7E0ECF3 CRC64;
MSEGTNYTPS QQAVIAQQGN NLLVSASAGS GKTTVLIERI MRKILAGTNI DQLLVVTFTN
LAAKHMKQKL ESQINKRISQ LMTDNPAQTT ASPAIRQLRQ QLNLLGVANI STLDAFCLRV
IQRYYYVIDL DPVFRLLTDN TEGLLIRDQV WDSVREQLYD EDGTLFDLLT ANFSNDRSDD
GLSRLIFRLF DFAQSTPDPE GWLSKLPEPY QVDEEALTAS AFYQQQLLPL LINEVDGMQM
KLRQAQKVAT DADLVPKTLE VLATAQKTLT TLREQLATAS WNELRTQVAT FKIGALKKTY
KDEPEKTARA KTMGTFVDGV KKQMTTLSET YFAADEQQVL DIMVGAKSIV TELVDATKQF
KTAFAAEKRR RHVLDFSDLE HLTLQILTSD NESSQAALAQ LRAQFEEVMV DEYQDINSLQ
ESILQLLARD QPGNMFMVGD VKQSIYQFRL ADPLLFLHKY HDFGTNPEHG ERIVLAENFR
SVANVDHLTN LIFSQLMDAP VGQIDYDEAA YLKYGPKDYP ADMPQTTNLL LYQTETDKST
EDVSTDETNF SIDDPAVGSI TMVAQKILAL KAQDKPIYER ANGEYRPFRY SDVALLVPTR
NHNLTIIDVF KRYHIPVVVN DAQNYFQTTE IRIMMALLSI IDNPYQDIPL VAVLRSPIVG
LDENQLVYLR IQNKTSDYFQ AVQDFYHHYP TGPATEYGDQ VYAKIETFLT QLTEFRDLAR
RNQIVTLIWQ IYERTGFLDY VGGMPAGKQR QANLHALYER AYTYEQGSFK GLFQFVRFIR
RMQEKDQDLA AAVAETDAEA VNVMTIHGSK GLEYPVIFVM DMDKQFNQTD TRSSALLDRE
AGIGITYLDP ETRVKYPTLP QVVTKQVVSK KMRAEEMRKL YVAMTRAQQQ LYLVGTIKDI
DTATEKWRQA FASDGRVLSA QARIAATNQL DWVGMAVMRH PAMKAYWGDE WPNYELTDDP
TKLAVELGDA TTVLAEQSEA VTDTQPDALE LVEQQVTIDV DQVTRDYLTT LLNFHYPHQA
STTTTAYQSV SEVKRIFEDP DTPLMNANPT VSQRRSRPTS RFVTGSLELP QFLQTTQAPT
SAEVGSATHL VLEQLDLTKP VNETTVQSTI DGLVANRVLT TNVAKLIAVD TIVAFYQSAL
GSQILDNPAK VHREVPFSLL IPARQIFDVD EHETGKVLIH GIIDGYLETA DGLTLFDYKT
DHVKRPEDLK TRYAGQVKLY AAALASMYPG KVTKQYLYSL PQQVFVPVN
