ADDA_LEUCK
ID ADDA_LEUCK Reviewed; 1236 AA.
AC B1MZM2;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=ATP-dependent helicase/nuclease subunit A {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01451};
DE AltName: Full=ATP-dependent helicase/nuclease AddA {ECO:0000255|HAMAP-Rule:MF_01451};
GN Name=addA {ECO:0000255|HAMAP-Rule:MF_01451}; Synonyms=rexA;
GN OrderedLocusNames=LCK_01147;
OS Leuconostoc citreum (strain KM20).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Leuconostoc.
OX NCBI_TaxID=349519;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KM20;
RX PubMed=18281406; DOI=10.1128/jb.01862-07;
RA Kim J.F., Jeong H., Lee J.-S., Choi S.-H., Ha M., Hur C.-G., Kim J.-S.,
RA Lee S., Park H.-S., Park Y.-H., Oh T.K.;
RT "Complete genome sequence of Leuconostoc citreum KM20.";
RL J. Bacteriol. 190:3093-3094(2008).
CC -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC and an ATP-dependent, dual-direction single-stranded exonuclease.
CC Recognizes the chi site generating a DNA molecule suitable for the
CC initiation of homologous recombination. The AddA nuclease domain is
CC required for chi fragment generation; this subunit has the helicase and
CC 3' -> 5' nuclease activities. {ECO:0000255|HAMAP-Rule:MF_01451}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- SUBUNIT: Heterodimer of AddA and AddB/RexB. {ECO:0000255|HAMAP-
CC Rule:MF_01451}.
CC -!- SIMILARITY: Belongs to the helicase family. AddA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01451}.
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DR EMBL; DQ489736; ACA82974.1; -; Genomic_DNA.
DR RefSeq; WP_012305309.1; NC_010471.1.
DR AlphaFoldDB; B1MZM2; -.
DR SMR; B1MZM2; -.
DR STRING; 349519.LCK_01147; -.
DR EnsemblBacteria; ACA82974; ACA82974; LCK_01147.
DR KEGG; lci:LCK_01147; -.
DR eggNOG; COG1074; Bacteria.
DR HOGENOM; CLU_001114_3_1_9; -.
DR OMA; KQSIYRW; -.
DR OrthoDB; 137860at2; -.
DR Proteomes; UP000002166; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 4.
DR Gene3D; 3.90.320.10; -; 1.
DR HAMAP; MF_01451; AddA; 1.
DR InterPro; IPR014152; DNA_helicase_suAddA.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR PANTHER; PTHR11070:SF48; PTHR11070:SF48; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
DR TIGRFAMs; TIGR02785; addA_Gpos; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Exonuclease; Helicase;
KW Hydrolase; Nuclease; Nucleotide-binding.
FT CHAIN 1..1236
FT /note="ATP-dependent helicase/nuclease subunit A"
FT /id="PRO_0000379293"
FT DOMAIN 3..474
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT DOMAIN 463..785
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT BINDING 24..31
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
SQ SEQUENCE 1236 AA; 140275 MW; CFC227C08925D520 CRC64;
MATRFTINQQ RAVEETGHNI LVAASAGSGK TTVLIERLIQ KILSGISVEN FLIVTFTNAA
AKEMRERLEI AIEKRLRDAN DGMKRFLQEQ LLLLPAANIS TIDAYALRLI ENYYHVIGLD
PQFRLLSDTA ERDMMRQDVL DTVFEAFYDE THPQHENFIA LVNNFGNPNQ DDALRQAVLK
LSDFAEARPD GQQWLKELDK NDLATANGLT QSQTYRLTIL PMMIARLEQL LEIAQATQTN
LSGVPELKKT QQAFDEIIAY LVNVQQALVD DDWDTLRGKL SQLPKVSLET KRSKGIKDEP
ELLALLAQAT TVKNNILGAK SDLKNVIDTF FKFDQNDWQY VEKQATELVN TLVTLTQACQ
SAFTTAKRDA QLVDFPDLGA LALEILNDDM TRKTVSGQFQ EILVDEYQDI NQLQETLLTK
VSNGHNMYMV GDVKQSIYGF RQAEPTLFAR KYRQFALPDN PDSRIELADN FRSQNNVTRV
TNLIFTQLMD EKLGDIAYTG EAKLVPKANY PTDVTPVFNM TIIKQPNVTT SEDEDESQEF
EKRQAQYAQL ANQILSLRDT TIFDRKAEPA GMRPVQYGDI AILTRSKTGY IDLVATLRQA
GIPTQVDSVG NYFQTMEVYL MLDVLRVIDN PHQDIPLAAI LRSPMFGYSE NDLAAVRLAD
KKHDYWSALT VFAQADQRSQ TVIDLIKKWH QLAIQNDLVA LIWAIFDDTS WLDYVAGMPG
GAQRQANLHA LYDYARTYQE NTHAGLFRFI RYIEQLQEND GQLGEAPQEA NEQAVRIMTI
HASKGLEFPI VFLPEFDKSF NTQDLSGKML IQKNAGIGLE YLQPEALVAM PTLKKLTVQQ
ALKRQSWSEE MRLLYVALTR AEQQIYVVGT VKLDENEENQ RLIALWQRAK ETSGQFLAED
LRLSAKSYFD WLLISLARTK NDDLESWLGD GHLPHVLGPE TTLDSQISVR FINESDVVIP
EPQGNETKEI TRASTYQAED FARAKAILDY QYPFQQATRT AAYQSVSEMK RLFEDPDRAN
LKTLNVLENG QLQPANELVS DKLVLPTFMT DGSQKPSSAA VGTATHLMLQ LLDFKQIQTH
QSLTALRDDL VTQGRLTAPV AALIDIDQIL NFLQTPFAQR LCLHADSLRR EATFAMIMPA
NQIYEHVHDQ ASVLVHGIID GYFIDKKTKS ITLFDYKTDF VRHGRVEEDI SKMMVRYRGQ
LTLYKQALMQ EFPDYTFYEP QLIILNVGRV IPITTK
