ID   DNAA_SPICI              Reviewed;         450 AA.
AC   P34028;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Chromosomal replication initiator protein DnaA;
GN   Name=dnaA;
OS   Spiroplasma citri.
OC   Bacteria; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae;
OC   Spiroplasma.
OX   NCBI_TaxID=2133;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 27556 / NCPPB 2647 / R8A2;
RX   PubMed=7764984; DOI=10.1007/bf01570187;
RA   Ye F., Renaudin J., Bove J.M., Laigret F.;
RT   "Cloning and sequencing of the replication origin (oriC) of the Spiroplasma
RT   citri chromosome and construction of autonomously replicating artificial
RT   plasmids.";
RL   Curr. Microbiol. 29:23-29(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 147-411.
RC   STRAIN=ATCC 27556 / NCPPB 2647 / R8A2;
RX   PubMed=8282191; DOI=10.1111/j.1574-6968.1993.tb06578.x;
RA   Suzuki K., Miyata M., Fukumura T.;
RT   "Comparison of the conserved region in the dnaA gene from three mollicute
RT   species.";
RL   FEMS Microbiol. Lett. 114:229-233(1993).
CC   -!- FUNCTION: Plays an important role in the initiation and regulation of
CC       chromosomal replication. Binds to the origin of replication; it binds
CC       specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-
CC       TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DnaA family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z19108; CAA79521.1; -; Genomic_DNA.
DR   EMBL; D14985; BAA03630.1; -; Genomic_DNA.
DR   PIR; S35732; S35732.
DR   RefSeq; WP_071890230.1; NZ_CP047437.1.
DR   AlphaFoldDB; P34028; -.
DR   SMR; P34028; -.
DR   STRING; 2133.SCITRI_001; -.
DR   PRIDE; P34028; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003688; F:DNA replication origin binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006270; P:DNA replication initiation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006275; P:regulation of DNA replication; IEA:UniProtKB-UniRule.
DR   CDD; cd06571; Bac_DnaA_C; 1.
DR   Gene3D; 1.10.1750.10; -; 1.
DR   Gene3D; 3.30.300.180; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00377; DnaA_bact; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR001957; Chromosome_initiator_DnaA.
DR   InterPro; IPR020591; Chromosome_initiator_DnaA-like.
DR   InterPro; IPR018312; Chromosome_initiator_DnaA_CS.
DR   InterPro; IPR013317; DnaA.
DR   InterPro; IPR013159; DnaA_C.
DR   InterPro; IPR038454; DnaA_N_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR010921; Trp_repressor/repl_initiator.
DR   PANTHER; PTHR30050:SF2; PTHR30050:SF2; 1.
DR   Pfam; PF00308; Bac_DnaA; 1.
DR   Pfam; PF08299; Bac_DnaA_C; 1.
DR   PRINTS; PR00051; DNAA.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00760; Bac_DnaA_C; 1.
DR   SUPFAM; SSF48295; SSF48295; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00362; DnaA; 1.
DR   PROSITE; PS01008; DNAA; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; DNA replication; DNA-binding; Nucleotide-binding.
FT   CHAIN           1..450
FT                   /note="Chromosomal replication initiator protein DnaA"
FT                   /id="PRO_0000114257"
FT   BINDING         146..153
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   450 AA;  51537 MW;  C401EF9B157FB961 CRC64;
     MNTKELWIEV KEILSRDESV SPEIYNYYIS DTNLYTVSDN NCLITTKSEI AIGVFEAGLN
     EKIKNILKKL TGIQYNISFE LEKNINKQAS VISKIDTLTE NNNLAYYENY TFENFVRGDS
     NHEAMQAALA VALDLGKKWN PLFIYGDSGL GKTHLLHAIE NKVNEIYKTN NRVKYLKADE
     FGKIAMDILN QGHEIIEAFK TSYDIYDCLL IDDIQLLAKR NKTNELFFHI FNSYIEKNKQ
     IVITSDKYPD DLGGFEARII SRFSYGLSIG LDSPDFETAL KILEQKLKHQ NNLGLFSEES
     LEFIALNFNS DVRKLEGAIK RLLFLAVMNK KPNEIITLAD VEKAFKNAPL QNNEKITPKK
     IKQIVADSYN ITIKAMMSKS RVSNVMQARQ LAMYFCRTLL DEPFTRIGTE FGGKDHTTVM
     NSVKKVEAHI STNKEFKHLV NAIRRKIEGR