ADDA_OCEIH
ID ADDA_OCEIH Reviewed; 1243 AA.
AC Q8ERW5;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=ATP-dependent helicase/nuclease subunit A {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01451};
DE AltName: Full=ATP-dependent helicase/nuclease AddA {ECO:0000255|HAMAP-Rule:MF_01451};
GN Name=addA {ECO:0000255|HAMAP-Rule:MF_01451}; OrderedLocusNames=OB1182;
OS Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC
OS 3954 / HTE831).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Oceanobacillus.
OX NCBI_TaxID=221109;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831;
RX PubMed=12235376; DOI=10.1093/nar/gkf526;
RA Takami H., Takaki Y., Uchiyama I.;
RT "Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge
RT and its unexpected adaptive capabilities to extreme environments.";
RL Nucleic Acids Res. 30:3927-3935(2002).
CC -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC and an ATP-dependent, dual-direction single-stranded exonuclease.
CC Recognizes the chi site generating a DNA molecule suitable for the
CC initiation of homologous recombination. The AddA nuclease domain is
CC required for chi fragment generation; this subunit has the helicase and
CC 3' -> 5' nuclease activities. {ECO:0000255|HAMAP-Rule:MF_01451}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- SUBUNIT: Heterodimer of AddA and AddB/RexB. {ECO:0000255|HAMAP-
CC Rule:MF_01451}.
CC -!- SIMILARITY: Belongs to the helicase family. AddA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01451}.
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DR EMBL; BA000028; BAC13138.1; -; Genomic_DNA.
DR RefSeq; WP_011065581.1; NC_004193.1.
DR AlphaFoldDB; Q8ERW5; -.
DR SMR; Q8ERW5; -.
DR STRING; 221109.22776864; -.
DR EnsemblBacteria; BAC13138; BAC13138; BAC13138.
DR KEGG; oih:OB1182; -.
DR eggNOG; COG1074; Bacteria.
DR HOGENOM; CLU_001114_3_1_9; -.
DR OMA; KQSIYRW; -.
DR OrthoDB; 137860at2; -.
DR PhylomeDB; Q8ERW5; -.
DR Proteomes; UP000000822; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 4.
DR Gene3D; 3.90.320.10; -; 1.
DR HAMAP; MF_01451; AddA; 1.
DR InterPro; IPR014152; DNA_helicase_suAddA.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR PANTHER; PTHR11070:SF48; PTHR11070:SF48; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
DR TIGRFAMs; TIGR02785; addA_Gpos; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Exonuclease; Helicase;
KW Hydrolase; Nuclease; Nucleotide-binding; Reference proteome.
FT CHAIN 1..1243
FT /note="ATP-dependent helicase/nuclease subunit A"
FT /id="PRO_0000379303"
FT DOMAIN 2..475
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT DOMAIN 502..803
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT BINDING 23..30
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
SQ SEQUENCE 1243 AA; 144384 MW; 3C4FDBC0112254C6 CRC64;
MVNWTKEQEE AIYTSGSDIL VAAAAGSGKT AVLVERIIQK LLAEHNPTNI DALLVVTFTN
AAAQEMRNRV GAALEQALAA NPSSIHLKKQ LSLLQRASIS TLHSFCLDIV KKNAYILDID
PSFRIADDME MDLLKQEVLD DLLEEWYGDS NPNQDSFFEV VNRFTSDRSD AEMEELIRSL
HTFAMQSPWP NQWLDKLVQT YNIPEEWTEE ELPWMNVLRD EVSRQLDAVS QEIDIAYSIT
QEADGPYHYA KTIEEEKAII QEALKNMESW KYLQQSMLQV KFGKLSGKKV DCDEEKKTKV
KKLRDSYKKR FQKMQEVWFA RNLDAHVYDM RVLAPVIKHL VELVKQFQDS FAKVKKEKAV
VDFSDLEHYC LQILIDESST EEEVIPSTVA MQLQRQFTEV LVDEYQDTNM VQETILQLIS
DRQGSGNMFM VGDVKQSIYR FRHAEPSLFI DKYKRFSNSD DPSIRIDLAR NFRSRENVLL
GANYIFRQLF DEDLGEISYD DAAELIYGNK MYDDHPLEAK PELLLIDNNS EDVEQSDNEE
TVEDLEKAQI EARAYAKQIK NWIGTPEDEI PMQVVDKATG KQRDLQYKDI VILMRSMTWA
STIADELQQQ GIPTYADLST GYFEAIEIKV MLSFLKVIDN PRQDIPLASI LRSPIIGLEE
DQLASIRLAD RKASFYEAVQ RYLQNEQDNS DTVKSLIRFM EMLNDFRQSA RQGSLSELIW
DIYRETGYYD FVGAIPGGKQ RQANLRALYD RARGYESTSF RGLYRFLRFI ERMEERGKDL
GAARALSEQE DVVRIMTIHK SKGLEFPIVI LGGMNKEFNQ RDLSEKYLLH KDLGFASKYI
DPVKRIQYPT LYYHAVRQAK FRDMLSEEIR VLYVALTRAK EKLLMVGTVP SIEKKLEKWQ
RIVDHPSWVL PAYFRMESKT YLDWVGPALL RHVGSEKIRT ENIGNQVLES INQDPSDWQI
TMNHALDYMD MKDSSMDNDD NLKEKVFNWE SVETEQEGLK NDVDARLSYE YAYKQATVFR
AKQSVTELKR QQEVKDDYSD QQILPPSHGP IGKRPNFLQK KLKLSPAEKG TALHTVMQHL
PMTHVLSEPE IEENIEKMVL EEKLTRMEAD SINIRAVERF FHTEIASKII SGNDMHREVP
FSIMLPASEV YANWKDQSNE KVLIQGVIDC LLQADTGWII LDYKTDFIED NPTSQKEQEL
IHRYQTQMTL YRRAIEQIWK QPVEQTYLYF FSQQLLIEVP YRD
