DNAB_BACSU
ID DNAB_BACSU Reviewed; 472 AA.
AC P07908;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1988, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Replication initiation and membrane attachment protein;
GN Name=dnaB; OrderedLocusNames=BSU28990;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTANTS DNABI AND DNABII.
RC STRAIN=168 / PY79;
RX PubMed=3027697; DOI=10.1073/pnas.84.3.653;
RA Hoshino T., McKenzie T., Schmidt S., Tanaka T., Sueoka N.;
RT "Nucleotide sequence of Bacillus subtilis dnaB: a gene essential for DNA
RT replication initiation and membrane attachment.";
RL Proc. Natl. Acad. Sci. U.S.A. 84:653-657(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3027671; DOI=10.1093/nar/14.24.9989;
RA Ogasawara N., Moriya S., Mazza P.G., Yoshikawa H.;
RT "Nucleotide sequence and organization of dnaB gene and neighbouring genes
RT on the Bacillus subtilis chromosome.";
RL Nucleic Acids Res. 14:9989-9999(1986).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969504; DOI=10.1099/13500872-142-11-3067;
RA Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J.,
RA Emmerson P.T., Harwood C.R.;
RT "The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis
RT chromosome containing genes responsible for stress responses, the
RT utilization of plant cell walls and primary metabolism.";
RL Microbiology 142:3067-3078(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9387221; DOI=10.1099/00221287-143-11-3431;
RA Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT "Sequencing and functional annotation of the Bacillus subtilis genes in the
RT 200 kb rrnB-dnaB region.";
RL Microbiology 143:3431-3441(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
CC -!- FUNCTION: Probable component of primosome involved in the initiation of
CC DNA replication. It is essential for both replication initiation and
CC membrane attachment of the origin region of the chromosome and plasmid
CC pUB110.
CC -!- MISCELLANEOUS: The two mutants dna-1 (dnaBI) and dnaB-19 (dnaBII) show
CC different characteristics for replication and membrane binding of
CC plasmid pUB110. DnaBI is essential for both chromosome and pUB110
CC replication, whereas dnaBII is necessary only for chromosome
CC replication.
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DR EMBL; M15183; AAA22404.1; -; Genomic_DNA.
DR EMBL; X04963; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; Z75208; CAA99604.1; -; Genomic_DNA.
DR EMBL; AF008220; AAC00358.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14859.1; -; Genomic_DNA.
DR PIR; B26580; B26580.
DR RefSeq; NP_390777.1; NC_000964.3.
DR RefSeq; WP_003229464.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; P07908; -.
DR SMR; P07908; -.
DR IntAct; P07908; 4.
DR STRING; 224308.BSU28990; -.
DR PaxDb; P07908; -.
DR EnsemblBacteria; CAB14859; CAB14859; BSU_28990.
DR GeneID; 937396; -.
DR KEGG; bsu:BSU28990; -.
DR PATRIC; fig|224308.179.peg.3148; -.
DR eggNOG; COG3611; Bacteria.
DR OMA; KIASHWA; -.
DR PhylomeDB; P07908; -.
DR BioCyc; BSUB:BSU28990-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.630; -; 1.
DR InterPro; IPR034829; DnaD-like_sf.
DR InterPro; IPR006343; DnaD_dom.
DR Pfam; PF07261; DnaB_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; DNA replication; DNA-binding; Nucleotide-binding; Primosome;
KW Reference proteome.
FT CHAIN 1..472
FT /note="Replication initiation and membrane attachment
FT protein"
FT /id="PRO_0000079950"
FT DNA_BIND 80..99
FT /evidence="ECO:0000305"
FT REGION 415..472
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 415..465
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 122..143
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000305"
FT BINDING 398..419
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000305"
FT MUTAGEN 113
FT /note="P->L: In DnaBI; required for chromosome and plasmid
FT replication."
FT MUTAGEN 379
FT /note="A->T: In DnaBII; required only for chromosome
FT replication."
SQ SEQUENCE 472 AA; 54890 MW; 8BDC5B2F6C67DB2E CRC64;
MADYWKDVLP VDPYVVKSRS MLQDIDRQII TQLYQPLIGP VAFSLYMTLW GELEQNRLWG
GESTHRQLMG MTQSNLKTIH QEQGKLEGIG LLKVYMKESE RQERLFIYEL LPPLRPNEFF
EDGMLNVFLY NRVGKTKYQQ LKQFFTHPAI SEDAKDITRP FNHAFESLQP SEWKLTSDME
ETVRLAEGSE YTSVGQSPSY TITEDVFDFD LFLAGLSETM IPRKAMTQQV RDTIKKLSYL
YGIDPLQMQN VVMSAIDERD VITTEALRKA ASDWYQIERN GQLPDLVEKT QPVHLREGEQ
PAEEDSLDGK LIALLEAISP KKLLQDIADG TEPSKADLKI IEEIMFEQKL EPGVTNVLIY
YVMLKTDMKL SKNYIQKIAS HWARKKVKTV REAMKLAIEE NRQYLEWAEG KTKSSKRNQK
VIREEKLPDW MTEKETASDS ESGQQKLHPQ DLEEQKKKMM EEMQKLKKYS AY
