ID   DNAB_BUCBP              Reviewed;         463 AA.
AC   Q89A52;
DT   16-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Replicative DNA helicase;
DE            EC=3.6.4.12;
GN   Name=dnaB; OrderedLocusNames=bbp_489;
OS   Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=224915;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bp;
RX   PubMed=12522265; DOI=10.1073/pnas.0235981100;
RA   van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F.,
RA   Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J.,
RA   Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A.;
RT   "Reductive genome evolution in Buchnera aphidicola.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003).
CC   -!- FUNCTION: Participates in initiation and elongation during chromosome
CC       replication; it exhibits DNA-dependent ATPase activity and contains
CC       distinct active sites for ATP binding, DNA binding, and interaction
CC       with DnaC protein, primase, and other prepriming proteins.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE016826; AAO27194.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q89A52; -.
DR   SMR; Q89A52; -.
DR   STRING; 224915.bbp_489; -.
DR   EnsemblBacteria; AAO27194; AAO27194; bbp_489.
DR   KEGG; bab:bbp_489; -.
DR   eggNOG; COG0305; Bacteria.
DR   HOGENOM; CLU_005373_0_3_6; -.
DR   OMA; IEFHARI; -.
DR   Proteomes; UP000000601; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   CDD; cd00984; DnaB_C; 1.
DR   Gene3D; 1.10.860.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR   InterPro; IPR007692; DNA_helicase_DnaB.
DR   InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR   InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR   InterPro; IPR016136; DNA_helicase_N/primase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00772; DnaB; 1.
DR   Pfam; PF03796; DnaB_C; 1.
DR   SUPFAM; SSF48024; SSF48024; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00665; DnaB; 1.
DR   PROSITE; PS51199; SF4_HELICASE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding; Primosome; Reference proteome.
FT   CHAIN           1..463
FT                   /note="Replicative DNA helicase"
FT                   /id="PRO_0000102018"
FT   DOMAIN          192..459
FT                   /note="SF4 helicase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT   BINDING         223..230
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
SQ   SEQUENCE   463 AA;  52984 MW;  E89C891396126031 CRC64;
     MKNVKKKNLD NQVEKLIELP NSLEAEQSIL GGLMLDNEQW DYISEKISEN DFFSLSHQLI
     FREMKYLLNK GYPIDLITLS ESLEQKGKLE YIGRFAYLAE LSKNVPSTAN ITTYADIVRE
     RSMVRKIIKI ANKIIQAGYD PRGKTSQELL NLAESKILSI SEQNFQKNSG PKNIEELLDI
     TLANIEKLFN TPYKGITGIN TGYQDLNNKT SGLQPSDLII IAARPSMGKT TFAMNICENI
     AMTYKKPVLI FSLEMSGEQI MMRMLSSLSR VNQEKLRTGQ LNDEDWARIS STINILLKKK
     NMYIDDSSTL TPSEMRSRSR KIYRENNGLS LIMVDYLQLI KVPSLIGNRT LEIAEISRML
     KSLAKELKIP IIALSQLNRS LEQRRDKRPI NSDLRESGSL EQDADLIMFI YRDELYHEHT
     DLKGIAEIII GKQRNGPIGT IKLTFNGHWS RFDNYSESKY SDE