ID   DNAB_HELPJ              Reviewed;         486 AA.
AC   Q9ZJM5;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Replicative DNA helicase;
DE            EC=3.6.4.12;
GN   Name=dnaB; OrderedLocusNames=jhp_1280;
OS   Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=85963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=J99 / ATCC 700824;
RX   PubMed=9923682; DOI=10.1038/16495;
RA   Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C.,
RA   Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J.,
RA   Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D.,
RA   Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J.;
RT   "Genomic sequence comparison of two unrelated isolates of the human gastric
RT   pathogen Helicobacter pylori.";
RL   Nature 397:176-180(1999).
CC   -!- FUNCTION: Participates in initiation and elongation during chromosome
CC       replication; it exhibits DNA-dependent ATPase activity and contains
CC       distinct active sites for ATP binding, DNA binding, and interaction
CC       with DnaC protein, primase, and other prepriming proteins.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE001439; AAD06865.1; -; Genomic_DNA.
DR   PIR; F71825; F71825.
DR   RefSeq; WP_000349702.1; NZ_CP011330.1.
DR   AlphaFoldDB; Q9ZJM5; -.
DR   SMR; Q9ZJM5; -.
DR   STRING; 85963.jhp_1280; -.
DR   EnsemblBacteria; AAD06865; AAD06865; jhp_1280.
DR   KEGG; hpj:jhp_1280; -.
DR   PATRIC; fig|85963.30.peg.1288; -.
DR   eggNOG; COG0305; Bacteria.
DR   OMA; WENLAKC; -.
DR   Proteomes; UP000000804; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   CDD; cd00984; DnaB_C; 1.
DR   Gene3D; 1.10.860.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR   InterPro; IPR007692; DNA_helicase_DnaB.
DR   InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR   InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR   InterPro; IPR016136; DNA_helicase_N/primase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00772; DnaB; 1.
DR   Pfam; PF03796; DnaB_C; 1.
DR   SUPFAM; SSF48024; SSF48024; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00665; DnaB; 1.
DR   PROSITE; PS51199; SF4_HELICASE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding; Primosome.
FT   CHAIN           1..486
FT                   /note="Replicative DNA helicase"
FT                   /id="PRO_0000102024"
FT   DOMAIN          172..471
FT                   /note="SF4 helicase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT   BINDING         203..210
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
SQ   SEQUENCE   486 AA;  55540 MW;  79FBC5779A83624B CRC64;
     MDHLKHLQQL QNIERIVLSG IVLANHKIEE IHSVLEPSDF YYPPHGLFFE IALKLHEVNC
     PIDENFIRQK MPKDKQISED DLVAIFAASP IDNIEAYVEE IKNASIKRKL FTLANTIREQ
     ALESAQKSSD ILNAVEREVY ALLNGSTIEG FRGIKEVLES TMNLITENQR KGSLKVTGIP
     TGFVQLDNYT SGFNQGSLVI LGARPSMGKT SLMMNMVLSA LNDDRGVAVF SLEMSAEQLA
     LRALSDLTSI NMHDLESARL DDDQWENLAK CFDHLSQKKL FFYDKSYVRM DQIRLQLRKL
     KSQHKELGIA FIDYLQLMSG NKATKERHEQ IAEISRELKT LARELEIPII ALVQLNRSLE
     NRDDKRPILS DIKDSGGIEQ DADIVLFLYR GYIYQMRAED NKIDKLKKEG KVEEAQELHL
     KVNEERRIHK QNGSIEEAEI IVAKNRNGAT GTVYTRFNAP FTRYEDMPVD SHLEEGQETK
     FEMPTT