DNAB_HELPY
ID DNAB_HELPY Reviewed; 488 AA.
AC O25916;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Replicative DNA helicase;
DE EC=3.6.4.12;
GN Name=dnaB; OrderedLocusNames=HP_1362;
OS Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=85962;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700392 / 26695;
RX PubMed=9252185; DOI=10.1038/41483;
RA Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT "The complete genome sequence of the gastric pathogen Helicobacter
RT pylori.";
RL Nature 388:539-547(1997).
CC -!- FUNCTION: Participates in initiation and elongation during chromosome
CC replication; it exhibits DNA-dependent ATPase activity and contains
CC distinct active sites for ATP binding, DNA binding, and interaction
CC with DnaC protein, primase, and other prepriming proteins.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- INTERACTION:
CC O25916; O25916: dnaB; NbExp=6; IntAct=EBI-7529552, EBI-7529552;
CC -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC {ECO:0000305}.
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DR EMBL; AE000511; AAD08402.1; -; Genomic_DNA.
DR PIR; B64690; B64690.
DR RefSeq; NP_208154.1; NC_000915.1.
DR RefSeq; WP_000349722.1; NC_018939.1.
DR PDB; 3GXV; X-ray; 2.20 A; A/B=1-121, C=101-122, D=98-123.
DR PDB; 4A1F; X-ray; 2.50 A; A/B=152-488.
DR PDB; 4ZC0; X-ray; 6.70 A; A/B/C/D=1-488.
DR PDBsum; 3GXV; -.
DR PDBsum; 4A1F; -.
DR PDBsum; 4ZC0; -.
DR AlphaFoldDB; O25916; -.
DR SMR; O25916; -.
DR DIP; DIP-3388N; -.
DR IntAct; O25916; 4.
DR MINT; O25916; -.
DR STRING; 85962.C694_07030; -.
DR PaxDb; O25916; -.
DR EnsemblBacteria; AAD08402; AAD08402; HP_1362.
DR KEGG; hpy:HP_1362; -.
DR PATRIC; fig|85962.47.peg.1459; -.
DR eggNOG; COG0305; Bacteria.
DR OMA; WENLAKC; -.
DR PhylomeDB; O25916; -.
DR EvolutionaryTrace; O25916; -.
DR Proteomes; UP000000429; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IBA:GO_Central.
DR CDD; cd00984; DnaB_C; 1.
DR Gene3D; 1.10.860.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR InterPro; IPR007692; DNA_helicase_DnaB.
DR InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR InterPro; IPR016136; DNA_helicase_N/primase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00772; DnaB; 1.
DR Pfam; PF03796; DnaB_C; 1.
DR SUPFAM; SSF48024; SSF48024; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00665; DnaB; 1.
DR PROSITE; PS51199; SF4_HELICASE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; DNA replication; DNA-binding; Helicase;
KW Hydrolase; Nucleotide-binding; Primosome; Reference proteome.
FT CHAIN 1..488
FT /note="Replicative DNA helicase"
FT /id="PRO_0000102023"
FT DOMAIN 172..471
FT /note="SF4 helicase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT BINDING 203..210
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT HELIX 3..23
FT /evidence="ECO:0007829|PDB:3GXV"
FT HELIX 25..27
FT /evidence="ECO:0007829|PDB:3GXV"
FT HELIX 28..32
FT /evidence="ECO:0007829|PDB:3GXV"
FT HELIX 37..39
FT /evidence="ECO:0007829|PDB:3GXV"
FT HELIX 43..57
FT /evidence="ECO:0007829|PDB:3GXV"
FT HELIX 64..69
FT /evidence="ECO:0007829|PDB:3GXV"
FT STRAND 73..75
FT /evidence="ECO:0007829|PDB:3GXV"
FT HELIX 79..86
FT /evidence="ECO:0007829|PDB:3GXV"
FT HELIX 95..120
FT /evidence="ECO:0007829|PDB:3GXV"
FT HELIX 154..171
FT /evidence="ECO:0007829|PDB:4A1F"
FT TURN 172..174
FT /evidence="ECO:0007829|PDB:4A1F"
FT HELIX 184..190
FT /evidence="ECO:0007829|PDB:4A1F"
FT STRAND 198..203
FT /evidence="ECO:0007829|PDB:4A1F"
FT HELIX 209..222
FT /evidence="ECO:0007829|PDB:4A1F"
FT STRAND 226..234
FT /evidence="ECO:0007829|PDB:4A1F"
FT HELIX 236..248
FT /evidence="ECO:0007829|PDB:4A1F"
FT HELIX 252..257
FT /evidence="ECO:0007829|PDB:4A1F"
FT HELIX 262..277
FT /evidence="ECO:0007829|PDB:4A1F"
FT STRAND 280..283
FT /evidence="ECO:0007829|PDB:4A1F"
FT HELIX 290..303
FT /evidence="ECO:0007829|PDB:4A1F"
FT STRAND 307..316
FT /evidence="ECO:0007829|PDB:4A1F"
FT HELIX 321..326
FT /evidence="ECO:0007829|PDB:4A1F"
FT HELIX 331..345
FT /evidence="ECO:0007829|PDB:4A1F"
FT STRAND 349..354
FT /evidence="ECO:0007829|PDB:4A1F"
FT HELIX 357..361
FT /evidence="ECO:0007829|PDB:4A1F"
FT STRAND 362..364
FT /evidence="ECO:0007829|PDB:4A1F"
FT HELIX 369..371
FT /evidence="ECO:0007829|PDB:4A1F"
FT STRAND 372..374
FT /evidence="ECO:0007829|PDB:4A1F"
FT STRAND 383..389
FT /evidence="ECO:0007829|PDB:4A1F"
FT HELIX 391..407
FT /evidence="ECO:0007829|PDB:4A1F"
FT HELIX 408..410
FT /evidence="ECO:0007829|PDB:4A1F"
FT HELIX 412..431
FT /evidence="ECO:0007829|PDB:4A1F"
FT STRAND 434..445
FT /evidence="ECO:0007829|PDB:4A1F"
FT STRAND 451..458
FT /evidence="ECO:0007829|PDB:4A1F"
FT HELIX 459..461
FT /evidence="ECO:0007829|PDB:4A1F"
FT TURN 469..471
FT /evidence="ECO:0007829|PDB:4A1F"
SQ SEQUENCE 488 AA; 55726 MW; 6FF84C2DE4ED606F CRC64;
MDHLKHLQQL QNIERIVLSG IVLANHKIEE VHSVLEPSDF YYPPNGLFFE IALKLHEEDC
PIDENFIRQK MPKDKQIKEE DLVAIFAASP IDNIEAYVEE IKNASIKRKL FGLANTIREQ
ALESAQKSSD ILGAVEREVY ALLNGSTIEG FRNIKEVLES AMDLITENQR KGSLEVTGIP
TGFVQLDNYT SGFNKGSLVI IGARPSMGKT SLMMNMVLSA LNDDRGVAVF SLEMSAEQLA
LRALSDLTSI NMHDLESGRL DDDQWENLAK CFDHLSQKKL FFYDKSYVRI EQIRLQLRKL
KSQHKELGIA FIDYLQLMSG SKATKERHEQ IAEISRELKT LARELEIPII ALVQLNRSLE
NRDDKRPILS DIKDSGGIEQ DADIVLFLYR GYIYQMRAED NKIDKLKKEG KIEEAQELYL
KVNEERRIHK QNGSIEEAEI IVAKNRNGAT GTVYTRFNAP FTRYEDMPID SHLEEGQETK
VDYDIVTT
