ID   DNAB_MYCIT              Reviewed;         576 AA.
AC   Q9F5P4;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Replicative DNA helicase;
DE            EC=3.6.4.12;
DE   Contains:
DE     RecName: Full=Min DnaB intein;
DE   Flags: Fragment;
GN   Name=dnaB;
OS   Mycobacterium intracellulare.
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium avium complex (MAC).
OX   NCBI_TaxID=1767;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1442;
RA   Madiraju M.V.V.S., Yamamoto K., Rajagopalan M., Rutherford S.A.;
RL   Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Participates in initiation and elongation during chromosome
CC       replication; it exhibits DNA-dependent ATPase activity. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- PTM: This protein undergoes a protein self splicing that involves a
CC       post-translational excision of the intervening region (intein) followed
CC       by peptide ligation. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AF307984; AAG31144.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9F5P4; -.
DR   SMR; Q9F5P4; -.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR   CDD; cd00984; DnaB_C; 1.
DR   Gene3D; 3.10.28.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR   InterPro; IPR036844; Hint_dom_sf.
DR   InterPro; IPR027434; Homing_endonucl.
DR   InterPro; IPR006142; INTEIN.
DR   InterPro; IPR004042; Intein_endonuc.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF03796; DnaB_C; 1.
DR   PRINTS; PR00379; INTEIN.
DR   SUPFAM; SSF51294; SSF51294; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF55608; SSF55608; 1.
DR   PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR   PROSITE; PS51199; SF4_HELICASE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Autocatalytic cleavage; DNA replication; DNA-binding;
KW   Helicase; Hydrolase; Nucleotide-binding; Primosome; Protein splicing.
FT   CHAIN           <1..15
FT                   /note="Replicative DNA helicase, 1st part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000013273"
FT   CHAIN           16..351
FT                   /note="Min DnaB intein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000013274"
FT   CHAIN           352..576
FT                   /note="Replicative DNA helicase, 2nd part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000013275"
FT   DOMAIN          154..260
FT                   /note="DOD-type homing endonuclease"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00273"
FT   DOMAIN          313..576
FT                   /note="SF4 helicase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT   NON_TER         1
SQ   SEQUENCE   576 AA;  63530 MW;  6D59341A779D082F CRC64;
     RWADIIVAAR PGVGKALALD TPLPTPTGWT TTGDVAVGDE LLGADGKPTR VVAATEVLLG
     RPCYEVEFSD GTVIVADAAH QWLTETRASR KSAQAAAAGY NRYKNQRTFA AVRTTAEIAG
     TLRCPTLDRR LNHSVVNARA LDLPDREFLV PPYTLGAWLG DGTSAAAQIT AADPEIIMRI
     EAEMSSTVGT LQARLRTIGV LGNKHIPTEY LRGSETQRRE LLAGLLDTDG TVTAGGAVQF
     SVTNQRLACD VAELIVSLGY RCQTSTKRVR GRNESSSIAY TLTFSTEKKM FALERKAIHK
     ERRAMAGTTR SGSRFIVDVR PVETVAVRCV EVDNGSHLYL ASRAMVPTHN STLGLDFLRS
     CSIKHRMASV IFSLEMSKSE IVMRLLSAEA KIKLADMRSG RMSDEDWTRL ARRMSEISEA
     PLYIDDSPNL TMMEIRAKAR RLRQKADLRL VVVDYLQLMS SGKKVESRQL EVSEFSRQLK
     LLAKELEVPV VAISQLNRGP EQRTDKKPML SDLRESGSLE QDADMVILLN RPDAFERDDP
     RGGEADFILA KHRNGPTKTV TVAHQLHLSR FANMAR