ID   DNAB_NEOYE              Reviewed;         566 AA.
AC   Q1XDF3;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Probable replicative DNA helicase;
DE            EC=3.6.4.12;
DE   Contains:
DE     RecName: Full=Pye dnaB intein;
GN   Name=dnaB;
OS   Neopyropia yezoensis (Susabi-nori) (Pyropia yezoensis).
OG   Plastid; Chloroplast.
OC   Eukaryota; Rhodophyta; Bangiophyceae; Bangiales; Bangiaceae; Neopyropia.
OX   NCBI_TaxID=2788;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=U-51;
RA   Kunimoto M., Morishima K., Yoshikawa M., Fukuda S., Kobayashi T.,
RA   Kobayashi M., Okazaki T., Ohara I., Nakayama I.;
RT   "Whole genome sequence of Porphyra yezoensis chloroplast.";
RL   Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- PTM: This protein undergoes a protein self splicing that involves a
CC       post-translational excision of the intervening region (intein) followed
CC       by peptide ligation. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AP006715; BAE92458.1; -; Genomic_DNA.
DR   RefSeq; YP_537015.1; NC_007932.1.
DR   AlphaFoldDB; Q1XDF3; -.
DR   SMR; Q1XDF3; -.
DR   GeneID; 3978882; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   CDD; cd00984; DnaB_C; 1.
DR   Gene3D; 1.10.860.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR   InterPro; IPR007692; DNA_helicase_DnaB.
DR   InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR   InterPro; IPR016136; DNA_helicase_N/primase_C.
DR   InterPro; IPR003586; Hint_dom_C.
DR   InterPro; IPR036844; Hint_dom_sf.
DR   InterPro; IPR030934; Intein_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF03796; DnaB_C; 1.
DR   SMART; SM00305; HintC; 1.
DR   SUPFAM; SSF48024; SSF48024; 1.
DR   SUPFAM; SSF51294; SSF51294; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00665; DnaB; 1.
DR   PROSITE; PS50818; INTEIN_C_TER; 1.
DR   PROSITE; PS51199; SF4_HELICASE; 2.
PE   3: Inferred from homology;
KW   ATP-binding; Autocatalytic cleavage; Chloroplast; DNA replication;
KW   DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Plastid; Primosome;
KW   Protein splicing; Repeat.
FT   CHAIN           1..361
FT                   /note="Probable replicative DNA helicase, 1st part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000277258"
FT   CHAIN           362..509
FT                   /note="Pye dnaB intein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000277259"
FT   CHAIN           510..566
FT                   /note="Probable replicative DNA helicase, 2nd part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000277260"
FT   DOMAIN          156..426
FT                   /note="SF4 helicase; first part"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT   DOMAIN          308..566
FT                   /note="SF4 helicase; second part"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT   BINDING         187..194
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
SQ   SEQUENCE   566 AA;  64847 MW;  C557181FDB577549 CRC64;
     MLTQQSISLL KSVEKISPDF FYFTSTSLLY RAALENVNHA KTKSVRNFFI NLKNEKMIQD
     LNELNEVFSL IEQAPLSDTI SEYSAVVIDN YIKRLLLACG DSLCLISCSK QLIEQKNITS
     IVSQLTKAYE ILDEKDTQTL ATILANLLVH LDTRKKISIN SSILSGFTEL DSITQGFKKS
     DLIILAGRPS MGKTAFAINI TRYVINQKRS YVILFSLEMS TEQLLRRILA QECNLNGQKI
     QSGQLNNDEW QYVIQKSKTL ANLNLYIDDS AKISTDTIKT KVKFFKLQGK NIELIIIDYL
     QLLQESRQSD NRSQELSLIT RSLKILAKDL SLPILALSQL NRNLETRSDK RPLLSDLRES
     GCISKFNYLQ TSLYNQLQIL FNCYYKKIEV INFSAQKQQL FLAIKCSISK TGKKTVYKII
     TEAGKYIQLT SNHKLLTTQG WKRCDKINQN DMIAIQIKNF EEQKNVFNSF SSLTFESIQK
     INVTSLQIVF DLECKPLCNF ISNNFIVHNS IEQDADLVIM LYRESYYTQE TRSRDFTEVI
     IAKHRNGPTG TFQLKFNAQI AKFSDA