DNAB_NOSS1
ID DNAB_NOSS1 Reviewed; 879 AA.
AC Q8YZA1;
DT 11-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Replicative DNA helicase;
DE EC=3.6.4.12;
DE Contains:
DE RecName: Full=Endonuclease PI-AspHIP;
DE EC=3.1.-.-;
DE AltName: Full=Asp dnaB intein;
GN Name=dnaB; OrderedLocusNames=all0578;
OS Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX NCBI_TaxID=103690;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT "Complete genomic sequence of the filamentous nitrogen-fixing
RT cyanobacterium Anabaena sp. strain PCC 7120.";
RL DNA Res. 8:205-213(2001).
CC -!- FUNCTION: Participates in initiation and elongation during chromosome
CC replication; it exhibits DNA-dependent ATPase activity. {ECO:0000250}.
CC -!- FUNCTION: The intein is an endonuclease. {ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- PTM: This protein undergoes a protein self splicing that involves a
CC post-translational excision of the intervening region (intein) followed
CC by peptide ligation. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC {ECO:0000305}.
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DR EMBL; BA000019; BAB72536.1; -; Genomic_DNA.
DR PIR; AI1878; AI1878.
DR RefSeq; WP_010994754.1; NZ_RSCN01000009.1.
DR AlphaFoldDB; Q8YZA1; -.
DR SMR; Q8YZA1; -.
DR STRING; 103690.17129923; -.
DR MEROPS; N10.002; -.
DR EnsemblBacteria; BAB72536; BAB72536; BAB72536.
DR KEGG; ana:all0578; -.
DR eggNOG; COG0305; Bacteria.
DR eggNOG; COG1372; Bacteria.
DR OMA; RSHEKFV; -.
DR OrthoDB; 1709134at2; -.
DR Proteomes; UP000002483; Chromosome.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR CDD; cd00984; DnaB_C; 1.
DR Gene3D; 1.10.860.10; -; 1.
DR Gene3D; 3.10.28.10; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR InterPro; IPR007692; DNA_helicase_DnaB.
DR InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR InterPro; IPR016136; DNA_helicase_N/primase_C.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00772; DnaB; 1.
DR Pfam; PF03796; DnaB_C; 1.
DR Pfam; PF14528; LAGLIDADG_3; 1.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SUPFAM; SSF48024; SSF48024; 1.
DR SUPFAM; SSF51294; SSF51294; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF55608; SSF55608; 1.
DR TIGRFAMs; TIGR00665; DnaB; 1.
DR TIGRFAMs; TIGR01443; intein_Cterm; 1.
DR TIGRFAMs; TIGR01445; intein_Nterm; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
DR PROSITE; PS51199; SF4_HELICASE; 2.
PE 3: Inferred from homology;
KW ATP-binding; Autocatalytic cleavage; DNA replication; DNA-binding;
KW Endonuclease; Helicase; Hydrolase; Intron homing; Nuclease;
KW Nucleotide-binding; Primosome; Protein splicing; Reference proteome;
KW Repeat.
FT CHAIN 1..388
FT /note="Replicative DNA helicase, 1st part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000013270"
FT CHAIN 389..817
FT /note="Endonuclease PI-AspHIP"
FT /id="PRO_0000013271"
FT CHAIN 818..879
FT /note="Replicative DNA helicase, 2nd part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000013272"
FT DOMAIN 184..451
FT /note="SF4 helicase; first part"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT DOMAIN 502..651
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00273"
FT DOMAIN 614..878
FT /note="SF4 helicase; second part"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT BINDING 215..222
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
SQ SEQUENCE 879 AA; 98008 MW; E42CB186E2B26D11 CRC64;
MAEELSFQGD GSNRLPPQNI EAEEAILGGI LLDPEAIGRV SEVLITEAFY ISAHRDIYQA
ALRLHAQGKP TDLLSVTSWL TDNELLTRIG GRNKLATLVD RTVSAVNIDA LAGLVMEKYL
RRQLIKAGNE IVHLGYETEK ELPIVLDQAE QKVFGVTQER PQSGLVHIAD TLINNFQEIE
ERNQGIALPG IPCGFYDLDA MTSGFQRSDL IIVAGRPSMG KTAFCLNLAN NIAATMKLPV
AVFSLEMSKE QLVQRLLASE AQIESGYLRS GRLSQTQWEP LSRAISMLSE MPIFIDDTPN
ITVTQMRSQA RRLQAEQGVE LGLIVIDYLQ LMEGGGDNRV QELSKITRSL KGLARELSVP
VIALSQLSRG VEARTNKRPM LSDLRESGCL TGDSLVTLAP GGLQIPIREL VGKSGFAIWA
LNQATLKLEK AIVSNAFSTG VKPVFTLTTR KGCKIRATAN HKFLTIHGWR RLDELSIRQH
LCLPRHIPSF DKQTMTYAEV ALLGHLIGDG CTLPRHAIQY TTREIDLAEN VFFLAKEVFG
DAITPRISPE RSWYQVYLSA TQRLTRNLRN PVAKWLDSLG VFGLRSYEKF VPQELFSQPI
ELISCFLRHL WSTDGSIKLV GGKKPRPIAY YATSSYRLAV DVQTLLLKLG INATLKLVPQ
VGKGRNQYHV KITGKPDLDL FIKKVGAVGE YKLNSLQQIF EYLENCIHNT NRDIIPKDVW
KKIVVPAMQS VQLTTRELHS NIGTSYCGLA LYKANLSRER ALKVAEVVQS SELLTLAKSD
VYWDEIVSIE YSGEEEVFDL TVPGLHNFIA NNIVVHNSIE QDADLVIMLY RDDYYNSDTP
DRGLAEVIVA KHRNGPTGTV KLLFDPQFTK FKNLARSGY
