ID   DNAB_PASMU              Reviewed;         467 AA.
AC   Q9CNL6;
DT   05-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Replicative DNA helicase;
DE            EC=3.6.4.12;
GN   Name=dnaB; OrderedLocusNames=PM0412;
OS   Pasteurella multocida (strain Pm70).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Pasteurella.
OX   NCBI_TaxID=272843;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pm70;
RX   PubMed=11248100; DOI=10.1073/pnas.051634598;
RA   May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V.;
RT   "Complete genomic sequence of Pasteurella multocida Pm70.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001).
CC   -!- FUNCTION: Participates in initiation and elongation during chromosome
CC       replication; it exhibits DNA-dependent ATPase activity and contains
CC       distinct active sites for ATP binding, DNA binding, and interaction
CC       with DnaC protein, primase, and other prepriming proteins.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE004439; AAK02496.1; -; Genomic_DNA.
DR   RefSeq; WP_005726119.1; NC_002663.1.
DR   AlphaFoldDB; Q9CNL6; -.
DR   SMR; Q9CNL6; -.
DR   STRING; 747.DR93_1186; -.
DR   EnsemblBacteria; AAK02496; AAK02496; PM0412.
DR   GeneID; 62224662; -.
DR   KEGG; pmu:PM0412; -.
DR   HOGENOM; CLU_005373_0_0_6; -.
DR   OMA; IEFHARI; -.
DR   Proteomes; UP000000809; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   CDD; cd00984; DnaB_C; 1.
DR   Gene3D; 1.10.860.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR   InterPro; IPR007692; DNA_helicase_DnaB.
DR   InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR   InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR   InterPro; IPR016136; DNA_helicase_N/primase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00772; DnaB; 1.
DR   Pfam; PF03796; DnaB_C; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF48024; SSF48024; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00665; DnaB; 1.
DR   PROSITE; PS51199; SF4_HELICASE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding; Primosome; Reference proteome.
FT   CHAIN           1..467
FT                   /note="Replicative DNA helicase"
FT                   /id="PRO_0000102027"
FT   DOMAIN          193..461
FT                   /note="SF4 helicase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT   BINDING         224..231
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
SQ   SEQUENCE   467 AA;  52011 MW;  7BE33C8CA32AE87C CRC64;
     MAKKNAQIPS KPLEQGIVPP HSLEAEQSVL GGILIHPAHW DSISDMLIAD DFYLAAHRVI
     FQEMENLLRQ GKPIDLINLY EFLREKNLSE DVGGFAYLAE LSKNTPSVSN IVSYAAIVRD
     RAILRDLISV SNDVAQSCYT TKGMEVKDIL DEAERKVFAI SEKRTTSNEG PQNICAILEK
     TIDRIELLSK TEGHNGITGV STGFDALDKK TAGLQPSDLI IVAARPSMGK TTFAMNLCEN
     AALKSDKPVL VFSLEMPAEQ IMMRSLASLS RVDQTKIRTG QGLDQSDWSK IGSTMGLFAN
     KPNLYIDDSS GLTPTELRSR ARRVYRENGG LSLIMVDYLQ LMRAPGFADN RTLEIAEISR
     SLKALAKELE VPVIALSQLN RTLENRADKR PVNSDLRESG SIEQDADLIM FIYRDEVYNE
     NSEDKGVAEI IIGKQRNGPI GRVRLAFRGQ FSRFDNLAEQ RDIRDDY