ID   DNAB_PORPU              Reviewed;         568 AA.
AC   P51333;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Probable replicative DNA helicase;
DE            EC=3.6.4.12;
DE   Contains:
DE     RecName: Full=Ppu dnaB intein;
GN   Name=dnaB;
OS   Porphyra purpurea (Red seaweed) (Ulva purpurea).
OG   Plastid; Chloroplast.
OC   Eukaryota; Rhodophyta; Bangiophyceae; Bangiales; Bangiaceae; Porphyra.
OX   NCBI_TaxID=2787;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Avonport;
RA   Reith M.E., Munholland J.;
RT   "Complete nucleotide sequence of the Porphyra purpurea chloroplast
RT   genome.";
RL   Plant Mol. Biol. Rep. 13:333-335(1995).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- PTM: This protein undergoes a protein self splicing that involves a
CC       post-translational excision of the intervening region (intein) followed
CC       by peptide ligation. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC       {ECO:0000305}.
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DR   EMBL; U38804; AAC08219.1; -; Genomic_DNA.
DR   PIR; S73254; S73254.
DR   RefSeq; NP_053943.1; NC_000925.1.
DR   AlphaFoldDB; P51333; -.
DR   SMR; P51333; -.
DR   PRIDE; P51333; -.
DR   GeneID; 809968; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR   CDD; cd00984; DnaB_C; 1.
DR   Gene3D; 1.10.860.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR   InterPro; IPR007692; DNA_helicase_DnaB.
DR   InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR   InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR   InterPro; IPR016136; DNA_helicase_N/primase_C.
DR   InterPro; IPR003586; Hint_dom_C.
DR   InterPro; IPR003587; Hint_dom_N.
DR   InterPro; IPR036844; Hint_dom_sf.
DR   InterPro; IPR030934; Intein_C.
DR   InterPro; IPR006141; Intein_N.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00772; DnaB; 1.
DR   Pfam; PF03796; DnaB_C; 1.
DR   SMART; SM00305; HintC; 1.
DR   SMART; SM00306; HintN; 1.
DR   SUPFAM; SSF48024; SSF48024; 1.
DR   SUPFAM; SSF51294; SSF51294; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00665; DnaB; 1.
DR   PROSITE; PS50818; INTEIN_C_TER; 1.
DR   PROSITE; PS50817; INTEIN_N_TER; 1.
DR   PROSITE; PS51199; SF4_HELICASE; 2.
PE   3: Inferred from homology;
KW   ATP-binding; Autocatalytic cleavage; Chloroplast; DNA replication;
KW   DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Plastid; Primosome;
KW   Protein splicing; Repeat.
FT   CHAIN           1..361
FT                   /note="Probable replicative DNA helicase, 1st part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000013294"
FT   CHAIN           362..511
FT                   /note="Ppu dnaB intein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000013295"
FT   CHAIN           512..568
FT                   /note="Probable replicative DNA helicase, 2nd part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000013296"
FT   DOMAIN          156..424
FT                   /note="SF4 helicase; first part"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT   DOMAIN          308..568
FT                   /note="SF4 helicase; second part"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT   BINDING         187..194
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
SQ   SEQUENCE   568 AA;  65370 MW;  FAB74C09CF2791E3 CRC64;
     MLTQESEDLL KQIEKLSPDF FYFKSNSLVY RAILETVNPI DKIALVSLLT ALNTNNLIRQ
     LGRLETIMKL IENSPASNII YEYSKVILDN YVKRLLLKSG DSLCLISCSK KQITQSVITS
     VASQLTIAYE ILEDEGTYTL AEIFASLLVS LDTKKKISIN SGIFSGFWQL DLITNGFQKS
     DLIIIAGRPS MGKTAFAINI TRHIIKTSQY YVILFSLEMS TEQLLRRILA QECHLNSQKI
     QSGQLTNVEW QRIVEESKIL ANLNFYIDDS AEISCDIIKV KVKLLRLQGK KIKLIIIDYL
     QLLQESKKSE NRSQELSLIT RSLKILAREL NLPILVLSQL NRNLESRHNK RPLLSDLRES
     GCISKFSHIM WSHVSKPLFN FSIKKSHMHN FNKNIYQLLD QGEAFISRQD KKTTYKIRTN
     SEKYLELTSN HKILTLRGWQ RCDQLLCNDM ITTQIGFELS RKKKYLLNCI PFSLCNFETL
     ANINISNFQN VFDFAANPIP NFIANNIIVH NSIEQDADLV IMLYRESYYN KEMEMEDMTE
     IIVAKHRNGP LGTFQLKFDA NLANFLNV