ID   DNAB_RICFE              Reviewed;         494 AA.
AC   Q4UL62;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Replicative DNA helicase;
DE            EC=3.6.4.12;
GN   Name=dnaB; OrderedLocusNames=RF_0860;
OS   Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=315456;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-1525 / URRWXCal2;
RX   PubMed=15984913; DOI=10.1371/journal.pbio.0030248;
RA   Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E.,
RA   Parinello H., Claverie J.-M., Raoult D.;
RT   "The genome sequence of Rickettsia felis identifies the first putative
RT   conjugative plasmid in an obligate intracellular parasite.";
RL   PLoS Biol. 3:1-12(2005).
CC   -!- FUNCTION: Participates in initiation and elongation during chromosome
CC       replication; it exhibits DNA-dependent ATPase activity and contains
CC       distinct active sites for ATP binding, DNA binding, and interaction
CC       with DnaC protein, primase, and other prepriming proteins.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC       {ECO:0000305}.
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DR   EMBL; CP000053; AAY61711.1; -; Genomic_DNA.
DR   RefSeq; WP_011271188.1; NC_007109.1.
DR   AlphaFoldDB; Q4UL62; -.
DR   SMR; Q4UL62; -.
DR   STRING; 315456.RF_0860; -.
DR   EnsemblBacteria; AAY61711; AAY61711; RF_0860.
DR   KEGG; rfe:RF_0860; -.
DR   eggNOG; COG0305; Bacteria.
DR   HOGENOM; CLU_005373_0_2_5; -.
DR   OMA; IEFHARI; -.
DR   OrthoDB; 1709134at2; -.
DR   Proteomes; UP000008548; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   CDD; cd00984; DnaB_C; 1.
DR   Gene3D; 1.10.860.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR   InterPro; IPR007692; DNA_helicase_DnaB.
DR   InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR   InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR   InterPro; IPR016136; DNA_helicase_N/primase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00772; DnaB; 1.
DR   Pfam; PF03796; DnaB_C; 1.
DR   SUPFAM; SSF48024; SSF48024; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00665; DnaB; 1.
DR   PROSITE; PS51199; SF4_HELICASE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding; Primosome.
FT   CHAIN           1..494
FT                   /note="Replicative DNA helicase"
FT                   /id="PRO_0000281067"
FT   DOMAIN          198..490
FT                   /note="SF4 helicase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT   BINDING         229..236
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
SQ   SEQUENCE   494 AA;  55840 MW;  E7B7AC134C73C992 CRC64;
     MARNKINNDK NIAANEEADI PIPRVLPSNV QAEQMLLGAI LTNNELLSYV SEFLRSEHFF
     EPIHQKIYNA IEKITEKGLI ATPITLRSML TQDELFQEIE GAEYLAKLIT MSMMVINPVD
     YGKIIYDLAI KRNLINIGEE VVNDAYNSSL EIEAREQIEH AEAKLYDLAS ESLNEKSFTR
     IGIPISESLA SINRAMKNND HVIGISTGLL DLDNKLFGFH NSDLLILAGR PSMGKTAFAI
     NLALNACNNM RLKNIRDNQE IQSVGFFSLE MSSEQLTTRL LSMCAEIDST SLRTGILGEE
     KYNRLRKEAN TLSELQFFID DTPALSISAI RTRARRMKRK HNLGILFIDY LQLIRGVSKS
     ENRVSEISEI TQGLKAIAKE LNIPVIALSQ LSRAVELRED KKPMLSDLRE SGTIEQDADI
     VMFIYREEYY LTRKEPAAGD AKHAEWLDKL NKVYNIADII VAKHRNGPVG NVPLYYDSQF
     SKFGNLEKRT FSSN