ID   DNAB_RICPR              Reviewed;         486 AA.
AC   Q9ZD08;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Replicative DNA helicase;
DE            EC=3.6.4.12;
GN   Name=dnaB; OrderedLocusNames=RP542;
OS   Rickettsia prowazekii (strain Madrid E).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX   NCBI_TaxID=272947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Madrid E;
RX   PubMed=9823893; DOI=10.1038/24094;
RA   Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T.,
RA   Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H.,
RA   Kurland C.G.;
RT   "The genome sequence of Rickettsia prowazekii and the origin of
RT   mitochondria.";
RL   Nature 396:133-140(1998).
CC   -!- FUNCTION: Participates in initiation and elongation during chromosome
CC       replication; it exhibits DNA-dependent ATPase activity and contains
CC       distinct active sites for ATP binding, DNA binding, and interaction
CC       with DnaC protein, primase, and other prepriming proteins.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AJ235272; CAA14991.1; -; Genomic_DNA.
DR   PIR; E71658; E71658.
DR   RefSeq; NP_220915.1; NC_000963.1.
DR   RefSeq; WP_004597833.1; NC_000963.1.
DR   AlphaFoldDB; Q9ZD08; -.
DR   SMR; Q9ZD08; -.
DR   STRING; 272947.RP542; -.
DR   EnsemblBacteria; CAA14991; CAA14991; CAA14991.
DR   GeneID; 57569666; -.
DR   KEGG; rpr:RP542; -.
DR   PATRIC; fig|272947.5.peg.553; -.
DR   eggNOG; COG0305; Bacteria.
DR   HOGENOM; CLU_005373_0_2_5; -.
DR   OMA; IEFHARI; -.
DR   Proteomes; UP000002480; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   CDD; cd00984; DnaB_C; 1.
DR   Gene3D; 1.10.860.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR   InterPro; IPR007692; DNA_helicase_DnaB.
DR   InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR   InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR   InterPro; IPR016136; DNA_helicase_N/primase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00772; DnaB; 1.
DR   Pfam; PF03796; DnaB_C; 1.
DR   SUPFAM; SSF48024; SSF48024; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00665; DnaB; 1.
DR   PROSITE; PS51199; SF4_HELICASE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding; Primosome; Reference proteome.
FT   CHAIN           1..486
FT                   /note="Replicative DNA helicase"
FT                   /id="PRO_0000102029"
FT   DOMAIN          190..482
FT                   /note="SF4 helicase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT   BINDING         221..228
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
SQ   SEQUENCE   486 AA;  54940 MW;  B2514F7164644C5C CRC64;
     MVRNKINNDD NLSIPRVLPS NIQAEQMLLG AIITNNALLS YVSEFLRNEH FFEPIHQKIY
     DAIEKIIEKG LIATPITLRS MLTQDALFKE IEGEGYLAKL ITMSMMVINP IDYGKIIYDL
     AIKRNLINIG EEVVNNAYNS SLAVAAKEQI EYAEAKLYDL TKEGLNEKSF TKVGISISES
     LASINRAMKN NDHVIGISTG LLDLDNKLFG FHNSDLIILA GRPSMGKTAF AINLALNTCN
     NMRLKNIRDN QEIKSVGFFS LEMSSEQLTT RLLSLCAEID STALRTGMLS EDKYNRLRKE
     ANTLSELQFF IDDTPALSIS AIRTRARRMK RKHNLGILFI DYLQLIRGVS KSENRVNEIS
     EITQGLKAIA KELNIPVIAL SQLSRAVELR EDKKPMLSDL RESGTIEQDA DIVMFIYREE
     YYLTRKEPVA GDAKHAEWLD KLNKVYNIAD IIIAKHRNGP VGNVSLYYDS QFSKFGNLEK
     RTFNSI