DNAB_SYNY3
ID DNAB_SYNY3 Reviewed; 872 AA.
AC Q55418;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Replicative DNA helicase;
DE EC=3.6.4.12;
DE Contains:
DE RecName: Full=Ssp dnaB intein;
GN Name=dnaB; OrderedLocusNames=slr0833;
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27184 / PCC 6803 / N-1;
RX PubMed=8590279; DOI=10.1093/dnares/2.4.153;
RA Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N.,
RA Sugiura M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region
RT from map positions 64% to 92% of the genome.";
RL DNA Res. 2:153-166(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
RN [3]
RP CHARACTERIZATION OF INTEIN.
RX PubMed=9748659; DOI=10.1016/s0167-4838(98)00157-5;
RA Wu H., Xu M.Q., Liu X.Q.;
RT "Protein trans-splicing and functional mini-inteins of a cyanobacterial
RT dnaB intein.";
RL Biochim. Biophys. Acta 1387:422-432(1998).
RN [4]
RP CHARACTERIZATION OF INTEIN.
RX PubMed=10231563; DOI=10.1016/s0378-1119(99)00103-1;
RA Mathys S., Evans T.C. Jr., Chute I.C., Wu H., Chong S., Benner J.,
RA Liu X.Q., Xu M.Q.;
RT "Characterization of a self-splicing mini-intein and its conversion into
RT autocatalytic N- and C-terminal cleavage elements: facile production of
RT protein building blocks for protein ligation.";
RL Gene 231:1-13(1999).
CC -!- FUNCTION: Participates in initiation and elongation during chromosome
CC replication; it exhibits DNA-dependent ATPase activity. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- PTM: This protein undergoes a protein self splicing that involves a
CC post-translational excision of the intervening region (intein) followed
CC by peptide ligation.
CC -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BA000022; BAA10516.1; -; Genomic_DNA.
DR PIR; S75781; S75781.
DR PDB; 1MI8; X-ray; 2.00 A; A=379-478, A=771-811.
DR PDB; 6FRE; X-ray; 1.22 A; A=376-486.
DR PDB; 6FRG; X-ray; 1.53 A; A=376-486.
DR PDB; 6FRH; X-ray; 2.03 A; A/B=382-491.
DR PDBsum; 1MI8; -.
DR PDBsum; 6FRE; -.
DR PDBsum; 6FRG; -.
DR PDBsum; 6FRH; -.
DR AlphaFoldDB; Q55418; -.
DR SMR; Q55418; -.
DR IntAct; Q55418; 1.
DR STRING; 1148.1001271; -.
DR MEROPS; N10.002; -.
DR PaxDb; Q55418; -.
DR EnsemblBacteria; BAA10516; BAA10516; BAA10516.
DR KEGG; syn:slr0833; -.
DR eggNOG; COG0305; Bacteria.
DR eggNOG; COG1372; Bacteria.
DR InParanoid; Q55418; -.
DR OMA; RSHEKFV; -.
DR PhylomeDB; Q55418; -.
DR EvolutionaryTrace; Q55418; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IBA:GO_Central.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IBA:GO_Central.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR CDD; cd00984; DnaB_C; 1.
DR Gene3D; 1.10.860.10; -; 1.
DR Gene3D; 3.10.28.10; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR InterPro; IPR007692; DNA_helicase_DnaB.
DR InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR InterPro; IPR016136; DNA_helicase_N/primase_C.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00772; DnaB; 1.
DR Pfam; PF03796; DnaB_C; 1.
DR Pfam; PF14528; LAGLIDADG_3; 1.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SUPFAM; SSF48024; SSF48024; 1.
DR SUPFAM; SSF51294; SSF51294; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF55608; SSF55608; 1.
DR TIGRFAMs; TIGR00665; DnaB; 1.
DR TIGRFAMs; TIGR01443; intein_Cterm; 1.
DR TIGRFAMs; TIGR01445; intein_Nterm; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
DR PROSITE; PS51199; SF4_HELICASE; 2.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Autocatalytic cleavage; DNA replication;
KW DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Primosome;
KW Protein splicing; Reference proteome; Repeat.
FT CHAIN 1..380
FT /note="Replicative DNA helicase, 1st part"
FT /id="PRO_0000013288"
FT CHAIN 381..809
FT /note="Ssp dnaB intein"
FT /id="PRO_0000013289"
FT CHAIN 810..872
FT /note="Replicative DNA helicase, 2nd part"
FT /id="PRO_0000013290"
FT DOMAIN 176..443
FT /note="SF4 helicase; first part"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT DOMAIN 494..643
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00273"
FT DOMAIN 606..870
FT /note="SF4 helicase; second part"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT BINDING 207..214
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT STRAND 376..378
FT /evidence="ECO:0007829|PDB:6FRE"
FT STRAND 387..390
FT /evidence="ECO:0007829|PDB:6FRE"
FT TURN 391..394
FT /evidence="ECO:0007829|PDB:6FRE"
FT STRAND 395..398
FT /evidence="ECO:0007829|PDB:6FRE"
FT HELIX 399..402
FT /evidence="ECO:0007829|PDB:6FRE"
FT STRAND 409..414
FT /evidence="ECO:0007829|PDB:6FRE"
FT TURN 415..418
FT /evidence="ECO:0007829|PDB:6FRE"
FT STRAND 419..424
FT /evidence="ECO:0007829|PDB:6FRE"
FT STRAND 427..441
FT /evidence="ECO:0007829|PDB:6FRE"
FT STRAND 446..449
FT /evidence="ECO:0007829|PDB:6FRE"
FT STRAND 454..457
FT /evidence="ECO:0007829|PDB:6FRE"
FT STRAND 460..463
FT /evidence="ECO:0007829|PDB:6FRE"
FT HELIX 464..466
FT /evidence="ECO:0007829|PDB:6FRE"
FT STRAND 472..476
FT /evidence="ECO:0007829|PDB:6FRE"
FT STRAND 480..483
FT /evidence="ECO:0007829|PDB:6FRH"
FT TURN 485..488
FT /evidence="ECO:0007829|PDB:6FRH"
SQ SEQUENCE 872 AA; 97813 MW; AC97823A1CB85109 CRC64;
MAANPALPPQ NIEAEECILG GILLDPEAMG RIIDLLVVDA FYVKAHRLIY EAMLSLHGQS
QPTDLMSVSS WLQDHHHFEA IGGMVKLTQL LDRTISAVNI DRFAALIMDK YLRRQLIAAG
HDIVDLGYET SKELETIFDE SEQKIFRLTQ SRPQAGLVPL SETLVNTFIE LDKLHEKLSS
PGVETQFYDL DAMTGGLQRA DLIILAGRPS MGKTAFGLGI AANIAKNQNL PVAIFSLEMS
KEQLALRLVA SESLIDSNRL RTGHFSQAEF EPLTAAMGTL SSLPIYIDDT ASISVTQMRS
QVRRLQSEQK GPLGMVLIDY LQLMEGGSDN RVQELSKITR SLKGLAREIN APVIALSQLS
RAVESRTNKR PMMSDLRESG CISGDSLISL ASTGKRVSIK DLLDEKDFEI WAINEQTMKL
ESAKVSRVFC TGKKLVYILK TRLGRTIKAT ANHRFLTIDG WKRLDELSLK EHIALPRKLE
SSSLQLMSDE ELGLLGHLIG DGCTLPRHAI QYTSNKIELA EKVVELAKAV FGDQINPRIS
QERQWYQVYI PASYRLTHNK KNPITKWLEN LDVFGLRSYE KFVPNQVFEQ PQRAIAIFLR
HLWSTDGCVK LIVEKSSRPV AYYATSSEKL AKDVQSLLLK LGINARLSKI SQNGKGRDNY
HVTITGQADL QIFVDQIGAV DKDKQASVEE IKTHIAQHQA NTNRDVIPKQ IWKTYVLPQI
QIKGITTRDL QMRLGNAYCG TALYKHNLSR ERAAKIATIT QSPEIEKLSQ SDIYWDSIVS
ITETGVEEVF DLTVPGPHNF VANDIIVHNS IEQDADLIMM IYRDEYYNPD TPDPGVAELL
IVKHRNGPTG VVKLLFKPEF TQFLNLQRSN DY
