ID   DNAB_TREPA              Reviewed;         438 AA.
AC   O83097;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Replicative DNA helicase;
DE            EC=3.6.4.12;
GN   Name=dnaB; OrderedLocusNames=TP_0058;
OS   Treponema pallidum (strain Nichols).
OC   Bacteria; Spirochaetes; Spirochaetales; Treponemataceae; Treponema.
OX   NCBI_TaxID=243276;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Nichols;
RX   PubMed=9665876; DOI=10.1126/science.281.5375.375;
RA   Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G.,
RA   Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A.,
RA   Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D.,
RA   Khalak H.G., Richardson D.L., Howell J.K., Chidambaram M., Utterback T.R.,
RA   McDonald L.A., Artiach P., Bowman C., Cotton M.D., Fujii C., Garland S.A.,
RA   Hatch B., Horst K., Roberts K.M., Sandusky M., Weidman J.F., Smith H.O.,
RA   Venter J.C.;
RT   "Complete genome sequence of Treponema pallidum, the syphilis spirochete.";
RL   Science 281:375-388(1998).
CC   -!- FUNCTION: Participates in initiation and elongation during chromosome
CC       replication; it exhibits DNA-dependent ATPase activity. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- INTERACTION:
CC       O83097; O83047: dnaA; NbExp=2; IntAct=EBI-1582320, EBI-1582316;
CC       O83097; O83970: dnaX; NbExp=2; IntAct=EBI-1582320, EBI-1582351;
CC       O83097; O83089: TP_0050; NbExp=2; IntAct=EBI-1582320, EBI-1198251;
CC       O83097; O83130: TP_0092; NbExp=2; IntAct=EBI-1582320, EBI-1198269;
CC   -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE000520; AAC65054.1; -; Genomic_DNA.
DR   PIR; B71370; B71370.
DR   AlphaFoldDB; O83097; -.
DR   SMR; O83097; -.
DR   IntAct; O83097; 19.
DR   STRING; 243276.TPANIC_0058; -.
DR   EnsemblBacteria; AAC65054; AAC65054; TP_0058.
DR   KEGG; tpa:TP_0058; -.
DR   eggNOG; COG0305; Bacteria.
DR   HOGENOM; CLU_005373_0_2_12; -.
DR   OMA; IEFHARI; -.
DR   Proteomes; UP000000811; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   CDD; cd00984; DnaB_C; 1.
DR   Gene3D; 1.10.860.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR   InterPro; IPR007692; DNA_helicase_DnaB.
DR   InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR   InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR   InterPro; IPR016136; DNA_helicase_N/primase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00772; DnaB; 1.
DR   Pfam; PF03796; DnaB_C; 1.
DR   SUPFAM; SSF48024; SSF48024; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00665; DnaB; 1.
DR   PROSITE; PS51199; SF4_HELICASE; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding; Primosome; Reference proteome.
FT   CHAIN           1..438
FT                   /note="Replicative DNA helicase"
FT                   /id="PRO_0000102033"
FT   DOMAIN          185..438
FT                   /note="SF4 helicase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT   BINDING         216..223
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
SQ   SEQUENCE   438 AA;  47796 MW;  14F1D14AA29ACFFA CRC64;
     MPGMPNPTQE LKGKIPPHNL EAERAVLGAV LLDDSALSTA TEQLSASSFY SAAHQRIFQA
     LVELSDLGQR PDILVLSEHL RSCEALDFVG GSAYVASLTD AVPSAANVEY YTRIVCDAAM
     RRSLLKVARI ITAEAFNDTV SGNIVLETAQ REIYDLTNAR RVATFKLLKN LIPDLVNTIE
     TRYRNQSDLV GIATGLTALD NLTGGFQNSE LIVIGARPSM GKTALAMTMA SNIAIRQRIP
     TAFFSLEMSN LLLMQRLIAA ESGVSATNLR KGLLQLSDFG RIQNAAGEMY DAPLYIVDVP
     NMKLLDLRAV ARRLCVQEKI QIIFVDYLGL IVADNPFAPR YEQFAAISQS LKSLARELDI
     PIVALSQVGR PAEGSAPNLA DIRGSGAIEQ DADVVMFLHR DRNETETQLI LAKQRNGPIG
     TVELEFQASF TRFVCKSP