ID   DNAB_TRICV              Reviewed;         455 AA.
AC   P49519;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Probable replicative DNA helicase;
DE            EC=3.6.4.12;
GN   Name=dnaB;
OS   Trieres chinensis (Marine centric diatom) (Odontella sinensis).
OG   Plastid; Chloroplast.
OC   Eukaryota; Sar; Stramenopiles; Ochrophyta; Bacillariophyta; Mediophyceae;
OC   Biddulphiophycidae; Eupodiscales; Parodontellaceae; Trieres.
OX   NCBI_TaxID=1514140;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Kowallik K.V., Stoebe B., Schaffran I., Kroth-Pancic P., Freier U.;
RT   "The chloroplast genome of a chlorophyll a+c-containing alga, Odontella
RT   sinensis.";
RL   Plant Mol. Biol. Rep. 13:336-342(1995).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC       {ECO:0000305}.
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DR   EMBL; Z67753; CAA91729.1; -; Genomic_DNA.
DR   PIR; S78356; S78356.
DR   AlphaFoldDB; P49519; -.
DR   SMR; P49519; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   CDD; cd00984; DnaB_C; 1.
DR   Gene3D; 1.10.860.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR   InterPro; IPR007692; DNA_helicase_DnaB.
DR   InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR   InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR   InterPro; IPR016136; DNA_helicase_N/primase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00772; DnaB; 1.
DR   Pfam; PF03796; DnaB_C; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF48024; SSF48024; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00665; DnaB; 1.
DR   PROSITE; PS51199; SF4_HELICASE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chloroplast; DNA replication; DNA-binding; Helicase;
KW   Hydrolase; Nucleotide-binding; Plastid; Primosome.
FT   CHAIN           1..455
FT                   /note="Probable replicative DNA helicase"
FT                   /id="PRO_0000102035"
FT   DOMAIN          185..455
FT                   /note="SF4 helicase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT   BINDING         216..223
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
SQ   SEQUENCE   455 AA;  52320 MW;  36850758EE51EA81 CRC64;
     MEKINLKTGR LIVEKYLPHN FLAEKIVLSS LLISSEAIET CLRSLTIDAF YFKNHREIYK
     AILIMYKKNT PIDIVTLNTF LQNQGLLPKI GGIKVLIELV NQLPNLVYLE EYIRIIQDKY
     VRRSLIKLGY EAINSGYITN ISLEEILISL EKQMFSLTNE IKNQDVFSSV DLFSQILLEL
     KYKSSKPVLA GLSSGFYDLD SLTQGFQKSD LIIIAGRPSM GKTAFCLNIA TNIVKKYKLP
     ILFFSLEMSK EQLAYRLLSA EALINPMRLR NGHLNKKDWL KLHRIIKNLS SLPFFIDDTP
     NLSIQAIRSK VKKLLFEQNT IGLVVIDYLQ LMQSSTLKSS NRVQELSQIT RSLKNIAREF
     NVPVIALSQL SRNVENRIIK RPILSDLRES GSIEQDADLV LMLYRDQYYN SNNENDEQLD
     ITELILAKQR NGPIGTIQLK FDANYTKFFD YILKD