ID   DNAC_BACSU              Reviewed;         454 AA.
AC   P37469;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 2.
DT   03-AUG-2022, entry version 137.
DE   RecName: Full=Replicative DNA helicase;
DE            EC=3.6.4.12;
GN   Name=dnaC; OrderedLocusNames=BSU40440;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=7584024; DOI=10.1093/dnares/1.1.1;
RA   Ogasawara N., Nakai S., Yoshikawa H.;
RT   "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis
RT   chromosome containing the replication origin.";
RL   DNA Res. 1:1-14(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 177.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 425-454.
RC   STRAIN=168 / DE1;
RX   PubMed=1312531; DOI=10.1128/jb.174.6.1883-1890.1992;
RA   Maentsaelae P., Zalkin H.;
RT   "Cloning and sequence of Bacillus subtilis purA and guaA, involved in the
RT   conversion of IMP to AMP and GMP.";
RL   J. Bacteriol. 174:1883-1890(1992).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- INTERACTION:
CC       P37469; P06567: dnaI; NbExp=4; IntAct=EBI-2122822, EBI-2122847;
CC       P37469; O31435: ybdM; NbExp=3; IntAct=EBI-2122822, EBI-5255200;
CC   -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC       {ECO:0000305}.
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DR   EMBL; D26185; BAA05176.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB16081.2; -; Genomic_DNA.
DR   EMBL; M83690; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; S65970; S65970.
DR   RefSeq; NP_391924.2; NC_000964.3.
DR   RefSeq; WP_003226923.1; NZ_JNCM01000034.1.
DR   AlphaFoldDB; P37469; -.
DR   SMR; P37469; -.
DR   IntAct; P37469; 16.
DR   STRING; 224308.BSU40440; -.
DR   PaxDb; P37469; -.
DR   PRIDE; P37469; -.
DR   EnsemblBacteria; CAB16081; CAB16081; BSU_40440.
DR   GeneID; 937810; -.
DR   KEGG; bsu:BSU40440; -.
DR   PATRIC; fig|224308.179.peg.4377; -.
DR   eggNOG; COG0305; Bacteria.
DR   InParanoid; P37469; -.
DR   OMA; IEFHARI; -.
DR   PhylomeDB; P37469; -.
DR   BioCyc; BSUB:BSU40440-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IBA:GO_Central.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IBA:GO_Central.
DR   CDD; cd00984; DnaB_C; 1.
DR   Gene3D; 1.10.860.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR   InterPro; IPR007692; DNA_helicase_DnaB.
DR   InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR   InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR   InterPro; IPR016136; DNA_helicase_N/primase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00772; DnaB; 1.
DR   Pfam; PF03796; DnaB_C; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF48024; SSF48024; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00665; DnaB; 1.
DR   PROSITE; PS51199; SF4_HELICASE; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding; Primosome; Reference proteome.
FT   CHAIN           1..454
FT                   /note="Replicative DNA helicase"
FT                   /id="PRO_0000102034"
FT   DOMAIN          179..445
FT                   /note="SF4 helicase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT   BINDING         210..217
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00596"
FT   CONFLICT        177
FT                   /note="H -> Y (in Ref. 1; BAA05176)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        449
FT                   /note="Missing (in Ref. 4; M83690)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   454 AA;  50576 MW;  265FC916744C2A44 CRC64;
     MTDLLNDRLP PQNIEAEQAV LGAIFLQPSA LTLASEVLIP DDFYRMSHQK IYNAMLVLGD
     RGEPVDLVTV TSELANTDLL EEVGGISYLT DIANSVPTAA NIEYYAKIVE EKSILRRLIR
     TATTIAQDGY TREDEVEDLL SEAEKTIMEV AQRKNTSAFQ NIKDVLVQTY DNIEQLHNRK
     GDITGIPTGF TELDRMTAGF QRNDLIIVAA RPSVGKTAFA LNIAQNVATK TDESVAIFSL
     EMGAEQLVMR MLCAEGNINA QNLRTGNLTE EDWGKLTMAM GSLSNSGIYI DDTPGIRVSE
     IRAKCRRLKQ ESGLGMILID YLQLIQGSGR SKDNRQQEVS EISRELKSIA RELQVPVIAL
     SQLSRGVEQR QDKRPMMSDI RESGSIEQDA DIVAFLYRDD YYDKETENKN IIEIIIAKQR
     NGPVGTVSLA FVKEYNKFVN LERRFDDAGV PPGA